Scanning Electron Microscopy 2012
DOI: 10.5772/36701
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The Use of ESEM in Geobiology

Abstract: Today, an individual would be hard-pressed to find any science field that does not employ methods and instruments based on the use of fine focused electron and ion beams. Well instrumented and supplemented with advanced methods and techniques, SEMs provide possibilities not only of surface imaging but quantitative measurement of object topologies, local electrophysical characteristics of semiconductor structures and performing elemental analysis. Moreover, a fine focused e-beam is widely used for the creation … Show more

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Cited by 7 publications
(3 citation statements)
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References 57 publications
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“…This makes it possible to analyse very small spots, run line scans to see changes in elemental concentration along a particular section, or to undertake elemental mapping of an entire area on the sample (Pollard et al 2007, 112 over traditional SEM which, as described above, requires high vacuum around the sample being analysed, as well as conductive samples to reduce electron charging effects. Instead, the sample chamber in the ESEM allows the specimen to be surrounded by water vapour or gas in low vacuum, allowing for much greater flexibility (Doehne 2006, Thiel 2006, Ivarsson and Holmström 2012. This means that non-conductive samples can be imaged without prior coating, and that biological or dynamic processes can be observed in real time without desiccation or similar pre-treatment of the sample (see Rochow and Rochow 1978, 196 for different types of sample preparation).…”
Section: Scanning Electron Microscopy-energy Dispersive X-ray Spectromentioning
confidence: 99%
“…This makes it possible to analyse very small spots, run line scans to see changes in elemental concentration along a particular section, or to undertake elemental mapping of an entire area on the sample (Pollard et al 2007, 112 over traditional SEM which, as described above, requires high vacuum around the sample being analysed, as well as conductive samples to reduce electron charging effects. Instead, the sample chamber in the ESEM allows the specimen to be surrounded by water vapour or gas in low vacuum, allowing for much greater flexibility (Doehne 2006, Thiel 2006, Ivarsson and Holmström 2012. This means that non-conductive samples can be imaged without prior coating, and that biological or dynamic processes can be observed in real time without desiccation or similar pre-treatment of the sample (see Rochow and Rochow 1978, 196 for different types of sample preparation).…”
Section: Scanning Electron Microscopy-energy Dispersive X-ray Spectromentioning
confidence: 99%
“…In addition to electron interactions, X-rays are generated as the electron beam interacts with the specimen (see section 3. over traditional SEM which, as described above, requires high vacuum around the sample being analysed, as well as conductive samples to reduce electron charging effects. Instead, the sample chamber in the ESEM allows the specimen to be surrounded by water vapour or gas in low vacuum, allowing for much greater flexibility (Doehne 2006, Thiel 2006, Ivarsson and Holmström 2012. This means that non-conductive samples can be imaged without prior coating, and that biological or dynamic processes can be observed in real time without desiccation or similar pre-treatment of the sample (see Rochow and Rochow 1978, 196 for different types of sample preparation).…”
Section: Scanning Electron Microscopy-energy Dispersive X-ray Spectro...mentioning
confidence: 99%
“…The environmental scanning electron microscope (ESEM) is utilized for various geologic applications as it is well suited for dealing with the charging problems of uncoated specimens [1], the often wet nature of specimens, and the hydrocarbon contamination when interacting with geologic specimens [2]. The ESEM allows for many different dynamic experiments as well.…”
mentioning
confidence: 99%