The use of cDNA microarray to identify differentially expressed labor-associated genes within the human myometrium during labor

Esplin, M. Sean; Fausett, M. Bardett; Peltier, Morgan R.; Hamblin, Steven; Silver, Robert M.; Branch, D. Ware; Adashi, Eli Y.; Whiting, David G.

doi:10.1016/j.ajog.2004.12.021

Cited by 89 publications

(71 citation statements)

References 32 publications

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“…It gives an overall picture of the changes that the uterine muscle undergoes in its adaptation to gestation. Two studies have described the changes in gene expression in the myometrium in preterm patients not in labor (PTNIL) versus patients at TNIL (Bethin et al 2003, Charpigny et al 2003 and two additional studies have described the changes in gene expression in the myometrium in patients at TNIL versus patients at term in labor (TIL) (Esplin et al 2005, Havelock et al 2005. Other studies could not be compiled directly, either because they did not provide sufficient information on the genes being studied (i.e.…”

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

“…Chemokine transcripts increase in myometrium at term, including CCL13 (also known as monocyte chemotactic protein-4; MCP-4), CCL19, CCL21, CXCR4 (neuropeptide Y receptor-like; NPYRL) and CXCR5 (Burkitt lymphoma receptor) (Bethin et al 2003, Charpigny et al 2003, Rehman et al 2003. At the time of labor, a selective increase in CXCL10 (Interferon-inducible protein-10), CCL8 and CCL13 is noted (Esplin et al 2005; see Table 2). IL-8, MCP-1 and RANTES are regulated by local growth factors and cytokines such as TNF-alpha, interferon-gamma, and IL-1.…”

Section: Cytokine-chemokines Interactionmentioning

confidence: 99%

“…Fas receptor) in term myometrium (Table 2) await consideration in the control of the uterine growth process. Elevated expression of TNFRS11B is observed in human myometrium with or without labor (Esplin et al 2005, Rehman et al 2003. A peak in uterine osteoprotegerin (i.e.…”

Section: Cytokine-chemokines Interactionmentioning

confidence: 99%

“…Functional genomics has arrived at the study of parturition in different species: mice (Bethin et al 2003, Salomonis et al 2005; rats (Girotti & Zingg 2003) and humans (Chan et al 2002, Marvin et al 2002a, 2002b, Tashima et al 2002, Bethin et al 2003, Charpigny et al 2003, Keelan et al 2003, Esplin et al 2005, Havelock et al 2005. Also in different tissues: myometrium (Aguan et al 2000, Chan et al 2002, Bethin et al 2003, Charpigny et al 2003, Esplin et al 2005, Havelock et al 2005, Salomonis et al 2005; primary cultures of human myometrial cells ; fetal membranes (Muhle et al 2001, Chen et al 2002, Marvin et al 2002a, 2002b, Tashima et al 2002, Keelan et al 2003 and trophoblasts (Chen et al 2002, Kato et al 2002.…”

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

“…Also in different tissues: myometrium (Aguan et al 2000, Chan et al 2002, Bethin et al 2003, Charpigny et al 2003, Esplin et al 2005, Havelock et al 2005, Salomonis et al 2005; primary cultures of human myometrial cells ; fetal membranes (Muhle et al 2001, Chen et al 2002, Marvin et al 2002a, 2002b, Tashima et al 2002, Keelan et al 2003 and trophoblasts (Chen et al 2002, Kato et al 2002. Various methodologies have been used: suppression subtractive hybridization (SSH) (Chan et al 2002) or microarrays for most of the previous cited studies.…”

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

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Gene and protein expression in the myometrium in pregnancy and labor

Breuiller-Fouché¹,

Germain²

2006

Reproduction

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Microarray technologies widen our comprehension of the major structural and metabolic transformations which affect the myometrium from the very beginning of pregnancy until parturition. The results are coherent with the mass of information which was accumulated previously, primarily on the basis of studies of selected critical factors. They highlight the activation of precise signaling pathways, some of which may have been previously under evaluated. The remodelling and maturation processes that the myometrium undergoes in pregnancy appear clearly as phenomena which last during the full course of gestation. Comparatively, the onset of labor is perhaps the phenomenon which remains the least well described by these methods of analysis. Nevertheless, genomic studies constitute a necessary first step of orientation and help establishing new links between the generic signaling pathways that are activated during the normal or pathological gestation. These studies also represent an indicative step that will have to be paralleled, in the future, with the results of the systematic proteomic analysis of the myometrium.

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Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

Section: Cytokine-chemokines Interactionmentioning

confidence: 99%

Section: Cytokine-chemokines Interactionmentioning

confidence: 99%

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

See 3 more Smart Citations

Gene and protein expression in the myometrium in pregnancy and labor

Breuiller-Fouché¹,

Germain²

2006

Reproduction

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Identification and validation of suitable reference genes for quantitative real-time PCR gene expression analysis in pregnant human myometrium

Arrowsmith

2021

Mol Biol Rep

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Accurate quantification of quantitative PCR (qPCR) data requires a set of stable reference genes (RGs) for normalisation. Despite its importance to mechanistic studies, no evaluation of RG stability has been conducted for pregnant human myometrium. A systematic search of the literature was performed to identify the most used RGs in human myometrial gene expression studies. The stability of these genes, and others, was then evaluated using geNorm and NormFinder algorithms, in samples of myometrium from singleton or twin pregnancies (n = 7 per group) delivering at term or preterm. The most frequently cited RGs were GAPDH, ACTB, B2M and 18s. There was strong agreement between algorithms on the most and least stable genes: Both indicated CYC1, YWHAZ and ATP5B were the most stably expressed. Despite being some of the most used RGs, B2M, 18s and ACTB expression was least stable and was too variable for use as accurate normalisation factors. Pairwise variation analysis determined that the optimal number of RGs for accurate normalisation is two. Validation of the choice of RGs by comparing relative expression of oxytocin receptors (OXTR) using the least stable 18s and B2M, with the most stable, CYC1 and YWHAZ, erroneously demonstrated significantly increased OXTR expression in myometrium in singleton pregnancies compared to twins. This study demonstrates the importance of appropriate RG selection for accurate quantification of relative expression in pregnant human myometrium qPCR studies. For normalisation, the geometric mean of CYC1 and YWHAZ or ATP5B is suggested. The use of ACTB, 18s and B2M, is not recommended.

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High-dimensional biology in obstetrics and gynecology: Functional genomics in microarray studies

Romero

Tromp

2006

American Journal of Obstetrics and Gynecology

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The use of cDNA microarray to identify differentially expressed labor-associated genes within the human myometrium during labor

Cited by 89 publications

References 32 publications

Gene and protein expression in the myometrium in pregnancy and labor

Gene and protein expression in the myometrium in pregnancy and labor

Identification and validation of suitable reference genes for quantitative real-time PCR gene expression analysis in pregnant human myometrium

High-dimensional biology in obstetrics and gynecology: Functional genomics in microarray studies

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