The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems

Kratasyuk, Valentina A.; Esimbekova, Elena N.; Gladyshev, Michail I.; Khromichek, E.B.; Кузнецов, А. М.; Ivanova, E. A.

doi:10.1016/s0045-6535(00)00177-6

Cited by 41 publications

(24 citation statements)

References 5 publications

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“…The measurements were carried out in four replications; SD values were calculated. Figure 1 Bioluminescence kinetics of the bacteria was studied in aqueous media in the presence of HTO and Am(NO 3 ) 3 . were chosen here to demonstrate the changes in bioluminescence kinetics.…”

Section: Luminous Bacteriamentioning

confidence: 99%

“…To compare the role of ROS in bioluminescence kinetics changes in solutions of 241 Am(NO 3 ) 3 and HTO, the peroxide concentrations were compared in the radionuclide solutions of different specific radioactivities (Figure 3).…”

Section: Peroxides In Solution Of 241 Am(no 3 ) 3 and In Htomentioning

confidence: 99%

“…Effects of 241 Am and 3 H on NADH solutions were studied. Figure 4 presents the decays of NADH concentration in the solutions of 241 Am(NO 3 ) 3 The slight decay of NADH concentration in the control sample is concerned with NADH autooxidation in aqueous solutions in the presence of molecular oxygen [45].…”

Section: Effects Of Radionuclides On Endogenous Reducer Nadhmentioning

confidence: 99%

“…In the late 1980s, the test was standardized in Germany as a method for pollutant detection. Later, it was modified by different researchers and adapted for their specific purposes [2][3][4][5][6]. Now, the bacterial bioluminescent assay is a traditional and important biotechnological application of the bioluminescence phenomenon [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Bioluminescence kinetic parameters (activation and inhibition times) in solutions of the radionuclides are compared; the role of peroxides and stability of endogenous reducer (NADH) are under consideration. 3 and tritiated water (HTO, radiochemical purity 98%) were used as sources of ionizing radiation.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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Effects of Americium-241 (241Am), alpha-emitting radionuclide of high specific radioactivity, and tritium (3H), beta-emitting radionuclide, on luminous bacteria Photobacterium phosphoreum were compared. Bioluminescence intensity served as a marker of bacterial physiological activity. Three successive stages in the bioluminescence response to 241Am and 3H were found under conditions of lowdose irradiation: (1) absence of effects, (2) activation, and (3) inhibition. They were interpreted in terms of bacterial response to stressfactor as stress recognition, adaptive response/syndrome, and suppression of physiological function (i.e. radiation toxicity). Times of bioluminescence activation (TBA) and inhibition (TBI) were suggested as parameters to characterize hormesis and toxic stages in a course of chronic low-dose irradiation of the microorganisms. Values of TBA and TBI of 241Am were shorter than those of 3H, revealing higher impact of alpha-irradiation (as compared to beta-irradiation) under comparable radiation doses. Increases of peroxide concentration and NADH oxidation rates in 241Am aquatic solutions were demonstrated; these were not found in tritiated water. The results reveal a biological role of reactive oxygen species generated in water solutions as secondary products of the radioactive decay. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha- and beta-emitting radionuclides in aquatic solutions.

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Section: Luminous Bacteriamentioning

confidence: 99%

Section: Peroxides In Solution Of 241 Am(no 3 ) 3 and In Htomentioning

confidence: 99%

Section: Effects Of Radionuclides On Endogenous Reducer Nadhmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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Application of Biosensors for Environmental Analysis

Farré

Pérez

Kantiani

et al. 2011

Biophysico‐Chemical Processes of Anthropogenic Organic Compounds in Environmental Systems

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Disposable luciferase‐based microfluidic chip for rapid assay of water pollution

et al. 2018

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In the present study, we demonstrate the use of a disposable luciferase-based microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve after the introduction of the water sample and thus activate bioluminescent reactions. Limits of detection for copper (II) sulfate, 1,3-dihydroxybenzene and 1,4-benzoquinone for the proposed microfluidic biosensor measured 3 μM, 15 mM, and 2 μM respectively, and these values are higher or close to the level of conventional environmental biosensors based on lyophilized bacteria. Approaches for entrapment of enzymes on poly(methyl methacrylate) (PMMA) plates using a gelatin scaffold and solvent bonding of PMMA chip plates under room temperature were suggested. The proposed microfluidic system may be used with some available luminometers and future portable luminescence readers.

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The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems

Cited by 41 publications

References 5 publications

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Application of Biosensors for Environmental Analysis

Disposable luciferase‐based microfluidic chip for rapid assay of water pollution

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