The use of BHK suspension cells for the production of foot and mouth disease vaccines

Radlett, P. J.

doi:10.1007/bfb0000677

Cited by 23 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the same time (in the early sixties) BHK-21 (C13) cells were established and in 1964 the commercial production of inactivated FMD (Foot and Mouth Disease) vaccine was commenced by using a suspension process (based on the work by Capstick et al (1962)). The Wellcome Foundation scaled this process up to a 2500 l scale Radlett et al 1985).…”

mentioning

confidence: 99%

Introduction to animal cell culture technology—past, present and future

Merten

2006

Cytotechnology

View full text Add to dashboard Cite

mentioning

confidence: 99%

Introduction to animal cell culture technology—past, present and future

Merten

2006

Cytotechnology

View full text Add to dashboard Cite

“…Vero cells have been used for the production of viral vaccines for humans for more than ten years (Montagnon et al, 1984), whereas BHK-21 C13 cells are used for the production of veterinary vaccines (e.g. Capstick et al, 1962, Radlett 1987) and rather recently also for the production of recombined proteins for human therapy (BOdeker et al, 1994). Although both cell lines grow well in the SFM, they are not very interesting for the production of influenza virus, because the maximal titres obtainable in perfusion cultures ranged only between 256 and 512 HAU.…”

Section: Discussionmentioning

confidence: 99%

Production of Influenza Virus in Cell Cultures for Vaccine Preparation

Merten

Hannoun

Manuguerra

et al. 1996

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Influenza virus strains of different types for use as an inactivated vaccine have been successfully grown in different cell lines. Increasing titres were obtained with BHK-2l IBRS, VERO and MDCK cells. Cultures in stationary flasks, in spinner cultures or in large bioreactor systems were tested and the optimal conditions were studied. MDCK cells grown in serum-free medium before and during the virus production phase were found to yield high titres in the presence of trypsin. Satisfactory results were obtained with egg-adapted strains of human and equine origin as well as with strains just isolated from human patients without any further passages in eggs or cell culture.

show abstract

“…BHK-21 cells are widely used in biotechnological production of therapeutics and veterinary vaccines particularly rabies (Perrin et al 1995;Kallel et al 2002), foot and mouth disease (Radlett et al 1985), and Aujeszky's disease (Gaurina Srček et al 2005). Proliferative effect of C-BE on primary cell cultures was also confirmed on BHK-21 [C-13] (data not showed) and further experiments were done with P-BE.…”

mentioning

confidence: 80%

Effect of porcine brain growth factor on primary cell cultures and BHK-21 [C-13] cell line

Srček

Radošević

Kniewald

et al. 2008

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Growth factors from neural tissues have been described as potent mitogens for a wide variety of mesoderm- and ectoderm-derived cells in vitro. We used porcine brain extract for in vitro testing of proliferation properties on primary ovarian cells, uterine cells, and cardiomyocytes in culture as well as for BHK-21 [C-13] cell line. The addition of this extract accelerates proliferation in all examined cultures. It also lowers serum requirement and shortens the cultivation period for BHK-21 [C-13] cells. Fibroblast growth factors from brain of different species, but not porcine, are already characterized and their proliferative effect proved. Therefore, we purified, determined, and confirmed the presence of basic fibroblast growth factor in porcine brain extract by Western blot analysis and showed its biological activity on BHK-21 [C-13] cells.

show abstract

The use of BHK suspension cells for the production of foot and mouth disease vaccines

Cited by 23 publications

References 29 publications

Introduction to animal cell culture technology—past, present and future

Introduction to animal cell culture technology—past, present and future

Production of Influenza Virus in Cell Cultures for Vaccine Preparation

Effect of porcine brain growth factor on primary cell cultures and BHK-21 [C-13] cell line

Contact Info

Product

Resources

About