The Use of Anti-Pfs 25 Monoclonal Antibody for Early Determination of Plasmodium falciparum Oocyst Infections in Anopheles gambiae: Comparison with the Current Technique of Direct Microscopic Diagnosis

Gouagna, Louis C.; Bonnet, Sarah; Gounoue, Racéline Kamkumo; Tchuinkam, Timoléon; Safeukui, Innocent; Verhave, J.P.; Eling, W.M.C.; Boudin, Christian

doi:10.1006/expr.1999.4414

Cited by 6 publications

(2 citation statements)

References 11 publications

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“…For each experimental group, at least 20 mosquitoes were dissected 2 days after feeding and their midguts were examined for young oocysts using the procedure previously described (Gouagna et al. 1999).…”

Section: Detection Of Early and Mature Oocyst Stagementioning

confidence: 99%

Stage‐specific effects of host plasma factors on the early sporogony of autologous Plasmodium falciparum isolates within Anopheles gambiae

Gouagna

Bonnet

Gounoue

et al. 2004

Tropical Med Int Health

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SummaryQuantitatively assessing the impact of naturally occurring transmission-blocking (TB) immunity on malaria parasite sporogonic development may provide a useful interpretation of the underlying mechanisms. Here, we compare the effects of plasma derived from 23 naturally infected gametocyte carriers (OWN) with plasma from donors without previous malaria exposure (AB) on the early sporogonic development of Plasmodium falciparum in Anopheles gambiae. Reduced parasite development efficiency was associated with mosquitoes taking a blood meal mixed with the gametocyte carriers' own plasma, whereas replacing autologous plasma with non-immune resulted in the highest level of parasite survival. Seven days after an infective blood meal, 39.1% of the gametocyte carriers' plasma tested showed TB activity as only a few macrogametocytes ingested along with immune plasma ended up as ookinetes but subsequent development was blocked in the presence of immune plasma. In other experiments (60.9%), the effective number of parasites declined dramatically from one developmental stage to the next, and resulted in an infection rate that was two-fold lower in OWN than in AB infection group. These findings are in agreement with those in other reports and go further by quantitatively examining at which transition stages TB immunity exerts its action. The transitions from macrogametocytes to gamete/zygote and from gamete/zygote to ookinete were identified as main targets. However, the net contribution of host plasma factors to these interstage parasite reductions was low (5-20%), suggesting that irrespective of the host plasma factors, mosquito factors might also lower the survival level of parasites during the early sporogonic development.

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Section: Detection Of Early and Mature Oocyst Stagementioning

confidence: 99%

Stage‐specific effects of host plasma factors on the early sporogony of autologous Plasmodium falciparum isolates within Anopheles gambiae

Gouagna

Bonnet

Gounoue

et al. 2004

Tropical Med Int Health

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“…For immunofluorescent detection of early oocysts on the basal surface of the midgut epithelium, midguts were dissected between 56 and 62 h post-bloodfeeding (pbf), and whole mounts of unfixed midguts prepared as previously described (Gouagna et al, 1999). …”

Section: Methodsmentioning

confidence: 99%

Ookinete destruction within the mosquito midgut lumen explains Anopheles albimanus refractoriness to Plasmodium falciparum (3D7A) oocyst infection

Baton

Ranford-Cartwright

2012

International Journal for Parasitology

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Graphical abstractHighlights► Development of a human malaria parasite is compared between two mosquito vector species. ► Ookinete formation within the midgut lumen is equivalent in both mosquito species. ► Plasmodium falciparum fails to produce oocyst infections in Anopheles albimanus. ► All ookinetes are destroyed within the midgut lumen of A. albimanus. ► Malaria parasite survival in all of the different midgut compartments is quantified.

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The development of Plasmodium falciparum in experimentally infected Anopheles gambiae (Diptera: Culicidae) under ambient microhabitat temperature in western Kenya

et al. 2004

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The Use of Anti-Pfs 25 Monoclonal Antibody for Early Determination of Plasmodium falciparum Oocyst Infections in Anopheles gambiae: Comparison with the Current Technique of Direct Microscopic Diagnosis

Cited by 6 publications

References 11 publications

Stage‐specific effects of host plasma factors on the early sporogony of autologous Plasmodium falciparum isolates within Anopheles gambiae

Stage‐specific effects of host plasma factors on the early sporogony of autologous Plasmodium falciparum isolates within Anopheles gambiae

Ookinete destruction within the mosquito midgut lumen explains Anopheles albimanus refractoriness to Plasmodium falciparum (3D7A) oocyst infection

The development of Plasmodium falciparum in experimentally infected Anopheles gambiae (Diptera: Culicidae) under ambient microhabitat temperature in western Kenya

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