The underlying molecular mechanism and identification of transcription factor markers for laryngeal squamous cell carcinoma

Abstract

The screening and treatment of laryngeal squamous cell carcinoma (LSCC) still perplexes clinicians, making it necessary to explore new markers. To this end, this research examined the underlying molecular mechanism of LSCC based on high-throughput datasets ( n = 249) from multiple databases. It also identified transcription factors (TFs) independently associated with LSCC prognosis. Through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses, differential expression genes of LSCC were deemed relevant to the extracellular matrix and its related structures or pathways, suggesting that the extracellular matrix plays an important role in LSCC. At the same time, several hub genes that may also have important roles in LSCC were identified via protein–protein interaction analysis, including CDC45, TPX2, AURKA, KIF2C, NUF, MUC1, MUC7, MUC4, MUC15, and MUC21 . Eight unreported LSCC prognostic TFs – BCAT1, CHD4, FOXA2, GATA6, HNF1A, HOXB13, MAFF, and TCF4 – were screened via Kaplan–Meier curves. Cox analysis determined for the first time that HOXB13 expression and gender were independently associated with LSCC prognosis. Compared to control tissues, elevated expression of HOXB13 was found in LSCC tissues (standardized mean difference = 0.44, 95% confidence interval [0.13–0.76]). HOXB13 expression also makes it feasible to screen LSCC from non-LSCC (area under the curve = 0.77), and HOXB13 may play an essential role in LSCC by regulating HOXB7 . In conclusion, HOXB13 may be a novel marker for LSCC clinical screening and treatment.

Identification of Novel Prognostic Markers Associated With Laryngeal Squamous Cell Carcinoma Using Comprehensive Analysis

“…( 59 ) and study from Mo et al. ( 60 ) both proved that HOXB7 may serve as an oncogene for HNSCC and LSCC. Our analysis of TCGA-HNSC-Larynx, GSE27020, GSE39366, and GSE127165 further confirmed the role of the potential prognostic marker of HOXB7 in LSCC and HNSCC.…”

“…To date, HOXB7 has been implicated to be aberrantly expressed in several types of cancers, including breast cancer (53), gliomas (54), gastric cancer (55), esophageal squamous cell carcinoma (56), intrahepatic cholangiocarcinoma (57), and cervical cancer (58). Furthermore, study from Wu et al (59) and study from Mo et al (60) both proved that HOXB7 may serve as an oncogene for HNSCC and LSCC. Our analysis of TCGA-HNSC-Larynx, GSE27020, GSE39366, and GSE127165 further confirmed the role of the potential prognostic marker of HOXB7 in LSCC and HNSCC.…”

Down-regulated long non-coding RNA LHFPL3 antisense RNA 1 inhibits the radiotherapy resistance of nasopharyngeal carcinoma via modulating microRNA-143-5p/homeobox A6 axis

“…Afterward, it was also predicted that miR-143-5p had a targeting relation with HOXA6. Multiple homeobox members have been identified as pivotal modulators in cancer progression, such as HOXB13, which was validated as novel marker for laryngeal squamous cell carcinoma clinical screening and treatment [ 31 ]. Moreover, cumulative reports have confirmed the regulatory role of HOXA6 in cancers.…”

Glycolysis related gene expression signature in predicting prognosis of laryngeal squamous cell carcinoma

“…RNA-sequence data and clinicopathologic information were downloaded from The Cancer Genome Atlas (TCGA) data portal ( https://portal.gdc.cancer.gov/ ) [ 14 ]. A total of 12 normal laryngeal tissues and 111 laryngeal carcinoma tissues were enrolled [ 15 ]. The clinical information of TCGA set are shown in Supplementary Table 1.…”