The Uganda study on HPV variants and genital cancers

Buonaguro, Franco M.; Tornesello, Maria Lina; Salatiello, Immacolata; Okong, Pious; Buonaguro, Luigi; Beth‐Giraldo, Elke; Biryahwaho, Benon; Sempala, S. D. K.; Giraldo, Gaetano

doi:10.1016/s1386-6532(00)00131-1

Cited by 38 publications

(26 citation statements)

References 30 publications

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“…In a more recent study by Chaouki et al [1998], it was found that 67.7% of cervical cancer samples collected in Morocco contained HPV 16. Preliminary data by Buonaguro et al [2000] showed a narrowing pattern of HPV type in more advanced cervical and penile lesions in patients from the Kyadondo region of Uganda. They attributed this pattern to infection with the variant, HPV 16 Af1, and suggested that mainly HPV 16Af1 patients are progressing to cancer.…”

Section: Discussionmentioning

confidence: 98%

High prevalence of HPV 16 in South African women with cancer of the cervix and cervical intraepithelial neoplasia

Kay

Soeters

Nevin

et al. 2003

Journal of Medical Virology

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Despite the high prevalence of cervical cancer and cervical neoplasias in South Africa, few studies have been performed in this region to establish which human papillomavirus (HPV) types are associated with the development of high-grade cervical intraepithelial neoplasia lesions and cervical cancer. To investigate these prevalence rates, punch biopsies were obtained from 56 women with cervical cancer and 141 women with histologically diagnosed cervical intraepithelial neoplasia 2 or 3 lesions. Nested polymerase chain reaction (PCR) using consensus degenerate PCR primers was performed for the detection of HPV DNA and HPV typing was done by restriction fragment length polymorphism. Forty-seven (94%) of the cervical cancer and 114 (88%) of the cervical intraepithelial neoplasia 2/3 biopsies were positive for HPV DNA. The prevalence rates of the HPV types detected in the cervical cancer biopsies were HPV 16 (82%), HPV 18, (10%), HPV 33 (10%), HPV 31 (2%), HPV 58 (2%), HPV 35 (2%), and HPV 59 (2%). The cervical intraepithelial neoplasia lesions contained HPV 16 (56.6%), HPV 33 (14%), HPV 31 (10.9%), HPV X (7%), HPV 52 (3.9), HPV 58 (3.1%), HPV 35 (2.3%), HPV 18 (1.6%), HPV 11 (0.8%). Five of the nine fragments that were not typed by the RFLP, designated HPV-X, were sequenced to give HPV6 (1/5), HPV 26 (2/5), HPV 68 (1/5), and candHPV 87 (1/5). HPV 58 was detected in one cervical cancer biopsy and four biopsies from cervical intraepithelial neoplasia grade 3 lesions and was shown to be a previously described variant [Williamson and Rybicki (1991) J. Med. Virol. 33:165-171]. In addition, a cervical intraepithelial neoplasia grade 2 lesion was shown to harbour HPV type HAN2294 (cand HPV 87). The results of this study indicate that cervical cancer and cervical intraepithelial neoplasia 2/3 are largely associated with HPV 16 infection in this group of South African women and, therefore, an effective HPV 16 based vaccine should prevent the development of cervical cancer in a large proportion of women from this region of South Africa.

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Section: Discussionmentioning

confidence: 98%

High prevalence of HPV 16 in South African women with cancer of the cervix and cervical intraepithelial neoplasia

Kay

Soeters

Nevin

et al. 2003

Journal of Medical Virology

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“…In one study that included 5 HPV16-positive penile carcinoma and 5 cervical neoplasia biopsies from Uganda the presence of Af1 HPV16 variants was observed in 100% of the cases. 35 No results have been published on the characterization of HPV16 variants in penile cancer from developed countries.In our study, we evaluated the distribution of HPV genotypes and HPV16 variants in a group of cases diagnosed with keratinizing squamous cell carcinoma (SCC), verrucous SCC and sarcomatoid SCC of the penis, who were retrieved from archives at two National Cancer Centers in north (Milan) and south (Naples) Italy. We report strong differences in the distribution of HPV16 variants in penile carcinoma, in comparison to cervical carcinoma from the corresponding geographical area, which may provide insight into the different mechanisms through which the penile carcinoma arise.…”

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confidence: 99%

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confidence: 99%

Human papillomavirus genotypes and HPV16 variants in penile carcinoma

Tornesello

Duraturo

Losito

et al. 2007

Intl Journal of Cancer

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The causative role of human papillomaviruses (HPV) and HPV16 variants has been extensively studied in uterine cervix dysplastic lesions and invasive carcinoma; few such studies, however, have been performed in penile tumors. We have investigated HPV genotype and HPV16 variant distribution on 41 penile cancer biopsies from Italian patients. Cases were extracted from the respective pathology departments databases of National Cancer Institutes in Naples and Milan. HPV sequences were detected by PCR and characterized by direct sequence analysis. Infections with certain types of human papillomaviruses (HPV) have been strongly and consistently associated with the development of cervical intraepithelial neoplasia (CIN) and cervical cancer worldwide. 1-5 The more than 40 HPV genotypes infecting the genital mucosa can be divided into 3 groups: 'high-riskÕ or 'carcinogenicÕ types (16,18,31,33,35,39,45,51,52,56, 58, 59) associated with a high relative risk of cervical cancer; 'low riskÕ viruses (6,11,40,42,43,44,54, 61, 70, 72, 81, 89) associated with benign epithelial proliferations in the genital area, but not associated with invasive cervical cancer; and a group of 6 types (26,53, 66, 68, 73, 82) that is classified as 'probably carcinogenicÕ since there is limited data associating these HPV types with cervical cancer. 6,7 Additionally, carcinogenic HPVs are involved in the development of a subset of other carcinomas arising from mucosal squamous epithelial cells including penile carcinoma. 8 Penile cancer is a relatively rare disease in Europe and United States with incidence rates varying from 0.5 to 1.5 per 100,000 men. In other parts of the world and particularly in developing countries, however, it is a common male cancer with an incidence of 2 to 5 per 100,000 men, constituting for example, 12-22% of all male cancers in countries like Brazil, Uganda and Puerto Rico, which are also communities with high rates of cervical neoplasia. 9 Epidemiological studies have indicated that HPVs are sexually transmitted pathogens infecting the male and female squamous epithelium of the anogenital tract with similar prevalence rates (28%) in symptom-less university students. 10 Moreover, sub-clinical penile lesions (flat penile lesions) are frequently found in male sexual partners of women with CIN, with HPV infection rates of 73% and genotype concordance of 36% within the couples. 11 The prevalence of HPV-related high-grade penile intraepithelial neoplasia, however, is very low suggesting that penile tissue is less prone to maintain persistent infection and to undergo neoplastic transformation. As for the cervical cancer pathogenesis, several additional risk factors have been reported, in conjunction with HPV infection, for the development of invasive penile cancer: (i) exogenous cofactors (lack of circumcision in childhood, phimosis, smoking, trauma), 12,13 (ii) host cofactors (genetic factors, immune response) 14,15 and (iii) viral cofactors (infection by specific types, viral load, viral integration status).The frequency of ...

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“…We could not investigate if HPV-16 L1 polymorphism was responsible for the lower concordance between assays of non-European variants, due to primer or probe mismatch with HPV-16 sequences, because this information is not yet available in our cohort. However, L1 sequence data are available from a limited number of isolates and four sites of polymorphism have been described in the probe sequence (nucleotides 6862, 6864, 6865, and 6868) in European, Asian American, and African variants (46,48,(55)(56)(57)(58). Three sites of polymorphism have been described in the area hybridizing with the upper primer (nucleotides 6567, 6582, and 6576) and could impede amplification with the HPV-16 L1 assay (57,59).…”

Section: Discussionmentioning

confidence: 99%