Covalent organic
frameworks (COFs) and Ag-doped COFs (AgCOFs) are
prepared by the polycondensation procedure and characterized by electron
microscopy and molecular spectral techniques. Their catalysis of the
Cu2O particle reaction of glucose (GL)–Cu(II) was
examined by resonance Rayleigh scattering (RRS), and AgCOFs were found
to exhibit the strongest catalysis. The melamine (ML) aptamers (AptML) can attach to the surface of AgCOF and inhibit its catalytic
activity. When melamine (ML) is added to this reacting solution, AptML–ML complexes are formed and the Apts are desorbed
from the surface of AgCOF. As the concentration of ML increased, the
catalytic activity of AgCOF increased and the RRS signal enhanced
due to the increase in Cu2O particles. When the ML concentration
was in the range of 0.79–13.2 nmol/L, the RRS intensity increased
linearly, with a detection limit of 0.72 nmol/L. When the Apts of
urea and bisphenol A (BPA) were replaced by the AptML,
66.7–1333 nmol/L urea and 0.33–2.7 nmol/L BPA, respectively,
could also be determined, with detection limits of 30.4 nmol/L urea
and 0.15 nmol/L BPA. Based on this, a new AgCOF amplification RRS
method was established.