The translation initiation factor, PeIF5B, from Pisum sativum displays chaperone activity

Suragani, Madhuri; Rasheedi, Sheeba; Hasnain, Seyed E.; Ehtesham, Nasreen Z.

doi:10.1016/j.bbrc.2011.09.085

Cited by 8 publications

(11 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutations in mammalian eIF5B show this factor may play multiple roles during initiation in vitro (Pisareva and Pisarev, 2014). Pea eIF5B has been biochemically characterized as a heat stable protein with potential properties of a chaperone and binds to GTP as expected (Rasheedi et al, 2010;Suragani et al, 2011).…”

Section: Eif5bmentioning

confidence: 99%

Mechanism of Cytoplasmic mRNA Translation

Browning

Bailey‐Serres

2015

The Arabidopsis Book

187

220

View full text Add to dashboard Cite

Protein synthesis is a fundamental process in gene expression that depends upon the abundance and accessibility of the mRNA transcript as well as the activity of many protein and RNA-protein complexes. Here we focus on the intricate mechanics of mRNA translation in the cytoplasm of higher plants. This chapter includes an inventory of the plant translational apparatus and a detailed review of the translational processes of initiation, elongation, and termination. The majority of mechanistic studies of cytoplasmic translation have been carried out in yeast and mammalian systems. The factors and mechanisms of translation are for the most part conserved across eukaryotes; however, some distinctions are known to exist in plants. A comprehensive understanding of the complex translational apparatus and its regulation in plants is warranted, as the modulation of protein production is critical to development, environmental plasticity and biomass yield in diverse ecosystems and agricultural settings.

show abstract

Section: Eif5bmentioning

confidence: 99%

Mechanism of Cytoplasmic mRNA Translation

Browning

Bailey‐Serres

2015

The Arabidopsis Book

187

220

View full text Add to dashboard Cite

show abstract

“…Mammalian eIF5B and eIF5 together stimulate 48S initiation complex formation, influencing initiation codon selection during ribosomal scanning (Pisareva and Pisarev, 2014). Biochemical studies of a putative eIF5B from pea reported that it bound GTP as expected, was highly heat stable, and had properties of a molecular chaperone (Rasheedi et al, 2010;Suragani et al, 2011). However, the possibility that eIF5B could differentially impact translation of specific mRNAs has not been investigated, and there are no data on the requirement for eIF5B in plants.…”

Section: Discussionmentioning

confidence: 99%

“…Mutations in the GTP binding domain of human eIF5B affected its ability to promote translation (Wilson et al, 1999), and in Drosophila, homozygous dIF2 mutants are lethal in larval stages (Carrera et al, 2000). The only report of eIF5B in plants is of PeIF5B from pea (Pisum sativum), which in addition to its role in translation initiation was reported to have high thermostability (up to 95°C) and the ability to prevent thermal aggregation and promote refolding of heat-labile proteins (Rasheedi et al, 2007(Rasheedi et al, , 2010Suragani et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Mutations in eIF5B Confer Thermosensitive and Pleiotropic Phenotypes via Translation Defects in Arabidopsis thaliana

et al. 2017

View full text Add to dashboard Cite

The conserved eukaryotic translation initiation factor 5B, eIF5B, is a GTPase that acts late in translation initiation. We found that an Arabidopsis thaliana mutant sensitive to hot temperatures 3 (hot3-1), which behaves as the wild type in the absence of stress but is unable to acclimate to high temperature, carries a missense mutation in the eIF5B1 gene (At1g76810), producing a temperature sensitive protein. A more severe, T-DNA insertion allele (hot3-2) causes pleiotropic developmental phenotypes. Surprisingly, Arabidopsis has three other eIF5B genes that do not substitute for eIF5B1; two of these appear to be in the process of pseudogenization. Polysome profiling and RNA-seq analysis of hot3-1 plants show delayed recovery of polysomes after heat stress and reduced translational efficiency (TE) of a subset of stress protective proteins, demonstrating the critical role of translational control early in heat acclimation. Plants carrying the severe hot3-2 allele show decreased TE of auxinregulated, ribosome-related, and electron transport genes, even under optimal growth conditions. The hot3-2 data suggest that disrupting specific eIF5B interactions on the ribosome can, directly or indirectly, differentially affect translation. Thus, modulating eIF5B interactions could be another mechanism of gene-specific translational control.

show abstract

“…Recently eIF5B is shown to play a critical role during cell-cycle transition and some specific developmental stages [7]. We further reported PeIF5B showing similar ligand binding properties as other eIF5B homologues by biophysical approach [8] and quite interestingly, PeIF5B displays chaperon activity similar to several other translation factors [9].…”

Section: Introductionmentioning

confidence: 92%