The tomato B-type cyclin gene, SlCycB2 , plays key roles in reproductive organ development, trichome initiation, terpenoids biosynthesis and Prodenia litura defense

Gao, Shenghua; Gao, Yubin; Xiong, Chuan; Yu, Gang; Chang, Jiang; Yang, Qing; Yang, Changxian; Ye, Zhibiao

doi:10.1016/j.plantsci.2017.05.006

Cited by 76 publications

(69 citation statements)

References 63 publications

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“…Its homologous protein in Arabidopsis (AT5G06270) was also found to interact with GL2 or co-repressor TOPLESS proteins (Wu and Citovsky, 2017b, a). However, as reported in a recent study, overexpression of SICyc82 resulted in non-trichome phenotype, while suppression of SICyc82 promoted trichomes formation in tomato (Gao et al, 2017). These inconsistent results raise the important questions: what is the function of SlCycB2 in trichome formation and why the mutation of woolly motif can promote trichome formation?…”

Section: Introductionsupporting

confidence: 68%

“…It is well known that ectopic expression of a constitutive active B-type cyclin promoted the unicellular trichome differentiate to multicellular in Arabidopsis (Schnittger et al, 2002, 2005). The SlCycB2 gene has been reported to be a hypothesis B-type cyclin gene participating in trichome formation in tomato (Yang et al, 2011; Gao et al, 2017). However, the function of SlCycB2 during trichome development has not been well-studied.…”

Section: Discussionmentioning

confidence: 99%

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NbCycB2 represses Nbwo activity via a negative feedback loop in the tobacco trichome developmemt

Yong

et al. 2019

Preprint

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The wo protein and its downstream gene, SlCycB2 have been demonstrated to regulate the trichome development in tomato. It was shown that only gain-of-function mutant form of wo, Wov (wo woolly motif mutant allele) could induce the increase of trichome density. However, it is still unclear the relationships between wo, Wov and SlCycB2 in trichome regulation. In this study, we demonstrated Nbwo (NbWov) directly regulated the expressions NbCycB2 by binding to the promoter of NbCycB2 and its genomic sequences. As a feedback regulation, NbCycB2 negatively regulates the trichome formation by repressing Nbwo activity at protein level. We further found that the mutations of Nbwo woolly motif could prevent repression of NbWov by NbCycB2, which results in the significant increase of active Nbwo proteins, trichome density and branches. Our results revealed a novel reciprocal mechanism between NbCycB2 and Nbwo during the trichome formation in Nicotiana benthamiana.HighlightNbCycB2 is specifically expressed in trichomes of Nicotiana benthamiana and represses the Nbwo activity via a negative feedback loop in tobacco trichome developmemt.

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Section: Introductionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

NbCycB2 represses Nbwo activity via a negative feedback loop in the tobacco trichome developmemt

Yong

et al. 2019

Preprint

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“…Cultivated Solanum lycopersicum (tomato) and its wild relatives produce seven types of morphologically distinct trichomes: types I–VII (Simmons and Gurr, ). The Woolly ( Wo ) gene, encoding an HD‐ZIP IV transcription factor, mainly regulates type‐I trichome formation through heterodimer formation with the B‐type cyclin SlCycB2 in tomato (Yang et al ., ; Gao et al ., ). Ectopic expression of Wo in Solanum tuberosum (potato) and tobacco leads to increased trichome density, implying that trichomes in these species may share a common regulatory network (Yang et al ., ,b).…”

Section: Introductionmentioning

confidence: 97%

Hair, encoding a single C2H2 zinc‐finger protein, regulates multicellular trichome formation in tomato

et al. 2018

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Trichomes originate from the epidermal cells of nearly all terrestrial plants, which are specialized unicellular or multicellular structures. Although the molecular mechanism regulating unicellular trichome formation has been extensively characterized, most of the genes essential for multicellular trichome formation remain unknown. In this study, we identified an associated locus on the long arm of chromosome 10 using a genome-wide association study (GWAS) on type-I trichomes of 180 diverse Solanum lycopersicum (tomato) accessions. Using map-based cloning we then cloned the key gene controlling the initiation of this type of trichome, named Hair (H), which encodes a single C2H2 zinc-finger protein. Transgenic experiments showed that hair-absent phenotype is caused by the deletion of the entire coding region of H. We identified three alleles of H containing several missense mutations and a nucleotide deletion, which result in amino acid substitutions and a reading frame shift, respectively. In addition, knockdown of H or Woolly (Wo) represses the formation of type-I trichomes, suggesting that both regulators may function as a heterodimer. Direct protein-protein interaction between them was further detected through pull-down and yeast two-hybrid assays. In addition, ectopic expression of H in Nicotiana tabacum (tobacco) and expression of its homologs from Capsicum annuum (pepper) and tobacco in tomato can trigger trichome formation. Taken together, these findings suggest that the H gene may be functionally conserved in multicellular trichome formation in Solanaceae species.

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“…In this study, we succeeded in isolating the key genes Trichome development is intimately related to the cell cycle [24]. It was reported that SlCycB2 plays a critical role in reproductive organ development, multicellular trichome initiation, and secondary metabolite biosynthesis [12]. In this study, CCNB did not change from S1-6, while APC11, CDC7, CDC47, CDC53, and CDC54 were up-regulated at S1, and CDK2 was down-regulated.…”

Section: Discussionmentioning

confidence: 78%

“…It has been reported that SlCycB2 plays a critical role in reproductive organ development, multicellular trichome initiation, secondary metabolite biosynthesis, and defense in tomato [12].…”

mentioning

confidence: 99%

Comparative transcriptomics analysis revealing flower trichome development during flower development in two Lonicera Japonica Thunb. cultivars using RNA-seq

Chang

2020

Preprint

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Background: Trichomes comprise specialized multicellular structures that have the capacity to synthesize and secrete secondary metabolites and protect plants from biotic and abiotic stresses. However, little is known about the trichome formation mechanism during flower development in Lonicera Japonica Thunb.Results: Here, we present a genome-wide comparative transcriptome analysis between two L. japonica cultivars, toward the identification of biological processes and functional gene activities that occur during flowering stage trichome development. In this study, the density and average lengths of flower trichomes were at their highest during three green periods. Using the Illumina RNA-Seq method, we obtained 134,304 unigenes, 33,733 of which were differentially expressed. In an analysis of 40 differentially expressed unigenes (DEGs) involved in trichome development, 29 of these were transcription factors. The DEGs analysis of plant hormone signal transduction indicated that plant growth and development may be independent of GA and CTK signaling pathways, and plant stress may be independent of JA and ET signaling pathways. We successfully isolated key genes involved in the floral biosynthesis of odors, tastes, colors, and plant hormones, and proposed biosynthetic pathways for sesquiterpenoid, triterpenoid, monoterpenoid, flavonoid, and plant hormones. Furthermore, 82 DEGs were assigned to cell cycles and 2,616 were predicted as plant resistance genes (PRGs).Conclusions: This study provides a comprehensive characterization of the expression profiles of flower development during the seven developmental stages of L. japonica, thereby offering valuable insights into the molecular networks that underly flower development in L. japonica.

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The tomato B-type cyclin gene, SlCycB2 , plays key roles in reproductive organ development, trichome initiation, terpenoids biosynthesis and Prodenia litura defense

Cited by 76 publications

References 63 publications

NbCycB2 represses Nbwo activity via a negative feedback loop in the tobacco trichome developmemt

NbCycB2 represses Nbwo activity via a negative feedback loop in the tobacco trichome developmemt

Hair, encoding a single C2H2 zinc‐finger protein, regulates multicellular trichome formation in tomato

Comparative transcriptomics analysis revealing flower trichome development during flower development in two Lonicera Japonica Thunb. cultivars using RNA-seq

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