The Tobraviruses

Harrison, B. D.; Robinson, David J.

doi:10.1016/s0065-3527(08)60097-4

Cited by 91 publications

(44 citation statements)

References 125 publications

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“…Recent work on PotLV (5) and TRV (10), and the emergence of PMTV in North America (19,37), has led to an interest in monitoring for these viruses. Sources of polyclonal antisera to PotLV and PMTV are limiting and, in the case of TRV, antisera will not reliably detect all infections due to the occasional loss of a coat protein encoding RNA (15). For TRV, the macroarray circumvents these issues and allows for the detection of all the viruses of particular interest in North America.…”

Section: Discussionmentioning

confidence: 99%

Macroarray Detection of Eleven Potato-Infecting Viruses and Potato spindle tuber viroid

Agindotan

Perry

2008

Plant Disease

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A macroarray was developed for the detection of 11 potato viruses and Potato spindle tuber viroid. The 11 viruses detected included those commonly found or tested for in North American potato seed certification programs: Alfalfa mosaic virus, Cucumber mosaic virus, Potato mop top virus, Potato leafroll virus, Potato latent virus, Potato virus A, Potato virus M, Potato virus S, Potato virus X, Potato virus Y, and Tobacco rattle virus. These viruses were detected using oligonucleotide 70-mer probes and labeled targets prepared by a random primed amplification procedure. Potato plants analyzed included those infected with 12 reference virus stocks and 36 field isolates. Results from the macroarray were entirely consistent with those obtained using a standard serological assay (enzyme-linked immunosorbent assay). Four isolates of Potato spindle tuber viroid, in mixed infection with one or more viruses, also were detected in the array, although strong hybridization signals required amplification with viroid-specific primers in combination with anchored-random primers. In individual plants, up to four viruses, or a viroid plus two viruses, were detected, with no apparent competition or inhibition. Macroarrays are a cost-effective approach to the simultaneous diagnostic detection of multiple pathogens from infected plants.

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Section: Discussionmentioning

confidence: 99%

Macroarray Detection of Eleven Potato-Infecting Viruses and Potato spindle tuber viroid

Agindotan

Perry

2008

Plant Disease

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“…Tobacco rattle virus (TRV) is a bi-partite, positive-sense, single-stranded RNA virus with one of the broadest host ranges among plant viruses (Harrison and Robinson, 1978;MacFarlane, 1999). VIGS vectors developed from TRV (Liu et al, 2002;Ratcliff et al, 2001) have been used for gene function characterization in many plant species (reviewed by MacFarlane, 2010).…”

Section: Introductionmentioning

confidence: 99%

Virus‐induced gene silencing for Asteraceae—a reverse genetics approach for functional genomics inGerbera hybrida

Deng

Elomaa

Nguyen

et al. 2012

Plant Biotechnology Journal

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SummaryVirus-induced gene silencing (VIGS) is a natural defence mechanism in plants which leads to sequence-specific degradation of viral RNA. For identifying gene functions, Tobacco rattle virus (TRV)-based VIGS has been applied for silencing of endogenous genes in many plant species. Gerbera hybrida (Asteraceae) has emerged as a novel model for studies in flower development and secondary metabolism. For this highly heterozygous species, functional studies have been conducted through reverse genetic methods by producing stable transgenic lines, which, however, is labour-intensive and time-consuming. For the development of TRVbased VIGS system for gerbera, and for the first time for an Asteraceaeous species, we screened several gerbera cultivars and optimized the agroinfiltration methods for efficient silencing. Gene fragments for gerbera phytoene desaturase (GPDS) and Mg-chelatase subunits (GChl-H and GChl-I), expressed from a TRV vector, induced silencing phenotypes in leaves, scapes, and involucral bracts indicating their feasibility as markers for green tissues. In addition, robust silencing symptoms were achieved in gerbera floral tissues by silencing the anthocyanin pathway gene for chalcone synthase (GCHS1) and a gerbera B-type MADS-box gene globosa (GGLO1), confirming the phenotypes previously observed in stable transgenic lines. Unexpectedly, photobleaching induced by GPDS and GChl-H or GChl-I silencing, or by the herbicide norflurazon, resulted in silencing of the polyketide synthase gene G2PS1, which has no apparent connections to carotenoid or chlorophyll biosynthesis. We have shown feasibility of VIGS for functional studies in gerbera, but our results also show that selection of the marker gene for silencing must be critically evaluated.

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“…Hence the disease is sometimes called corky ringspot, and may render entire crops unmarketable at relatively low levels of symptom expression (Brown & Sykes, 1973). TRV has a notably wide host range, infecting over 100 plant species in nature, including a number of important crop plants and a further 400 species under laboratory conditions (Harrison & Robinson, 1978).…”

Section: Introductionmentioning

confidence: 99%

Effects of systemic infections with Tobacco rattle virus on agronomic and quality traits of a range of potato cultivars

Dale¹,

Robinson²,

Todd³

2004

Plant Pathology

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Infection of several commercially important potato cultivars with Tobacco rattle virus (TRV) can result in systemic infection with M-type virus, which persists through generations of vegetative propagation, although spraing symptoms in the tubers rarely develop. In field trials of nine such cultivars it was shown that TRV infection can delay plant emergence and retard subsequent growth, reduce tuber yield and mean tuber weight, increase tuber numbers and the proportion of small tubers, increase the incidence of growth cracks and misshapen tubers, diminish dry matter content, and exacerbate after-cooking blackening. Each cultivar was affected differently, showing different combinations and degrees of these effects. Virus concentration in leaf extracts from individual plants was also highly variable. These, and other potato cultivars similarly susceptible to TRV, are grown on large areas of UK and European potato land. The implications of growing such cultivars for the seed, ware and processing sectors of the potato industry are discussed.

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The Tobraviruses

Cited by 91 publications

References 125 publications

Macroarray Detection of Eleven Potato-Infecting Viruses and Potato spindle tuber viroid

Macroarray Detection of Eleven Potato-Infecting Viruses and Potato spindle tuber viroid

Virus‐induced gene silencing for Asteraceae—a reverse genetics approach for functional genomics inGerbera hybrida

Effects of systemic infections with Tobacco rattle virus on agronomic and quality traits of a range of potato cultivars

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