The Terpolymer Produced by Azotobacter Chroococcum 7B: Effect of Surface Properties on Cell Attachment

Бонарцев, А. П.; Yakovlev, Sergey; Boskhomdzhiev, A.P.; Zharkova, I. I.; Bagrov, D. V.; Мышкина, В. Л.; Mahina, T.K.; Kharitonova, E. P.; Samsonova, Olga; Zernov, A.L.; Жуйков, В. А.; Efremov, Yuri M.; Voinova, V. V.; Бонарцева, Г. А.; Шайтан, К. В.

doi:10.1371/journal.pone.0057200

Cited by 30 publications

(21 citation statements)

References 43 publications

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“…The PHBV was synthesized from the Azotobacter chroococcum strain 7B by the precursor feeding approach as previously described . Briefly, the culture was grown in shaker flasks at 30 °C in Burk's medium with 50 m M sucrose as primary carbon source supplemented with 20 m M sodium valerate (to produce the PHBV copolymer) and 50 m M sodium acetate (to regulate the PHBV molecular weight).…”

Section: Methodsmentioning

confidence: 99%

Poly(hydroxybutyrate‐co‐hydroxyvalerate) and bovine serum albumin blend prepared by electrospinning

Pavlova

Bagrov

Kopitsyna

et al. 2017

J of Applied Polymer Sci

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Electrospinning is a method for the preparation of nanosized polymer fibers. Here, electrospinning is used to prepare a blend of a polyester, poly(hydroxybutyrate‐co‐hydroxyvalerate) (PHBV), and a globular protein, bovine serum albumin (BSA). The electrospun blend film is compared with a solution‐cast blend film and with single‐component electrospun films made of PHBV and BSA. In the electrospun blend films, BSA manifests itself as flat ribbons and a fine network formed from fibers less than 50 nm in diameter. The dissolution rate of BSA from the electrospun blended film is lower than from the solution‐cast one. The films are characterized using scanning electron microscopy, differential scanning calorimetry, and contact‐angle measurements. The obtained PHBV+BSA blend films have several emergent properties: a slow BSA dissolution rate, a fine BSA network, and unusual thermal behavior. Thus, the PHBV+BSA blend films introduce a new class of polymer–protein blends. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45090.

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Section: Methodsmentioning

confidence: 99%

Poly(hydroxybutyrate‐co‐hydroxyvalerate) and bovine serum albumin blend prepared by electrospinning

Pavlova

Bagrov

Kopitsyna

et al. 2017

J of Applied Polymer Sci

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“…Previous research findings into PHA/PEG biocompatibility have been contradictory. Some of the authors reported that copolymerization or blending with PEG improved PHA biocompatibility while others argued that the presence of PEG decreased PHA biocompatibility …”

Section: Resultsmentioning

confidence: 99%

A study of synthesis and properties of poly‐3‐hydroxybutyrate/diethylene glycol copolymers

Volova

Zhila

Kiselev

et al. 2016

Biotechnology Progress

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This study investigates synthesis of poly(3-hydroxybutyrate)/diethylene glycol copolymers (P3HB/DEG) by Cupriavidus eutrophus B-10646 cells as related to DEG concentration in the medium and the time when it is added to the culture of cells synthesizing P3HB. The study determines the limits of physiological effect of DEG on C. eutrophus cells, showing that at DEG concentrations above 30 g/L, it inhibits cell growth, decreasing cell concentration and total P3HB/DEG yield and inducing an increase in the degree of saturation of fatty acids in lipids of cell cytoplasmic membrane. A series of copolymers containing different molar fractions of DEG (between 0.13 and 3.0 mol%) have been synthesized and their physicochemical, physical/mechanical, and biological properties have been investigated as related to the chemical composition and proportions of DEG monomers of the polymers. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1017-1028, 2016.

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“…Количество живых клеток определяли по стандартной калибровочной прямой для теста ХТТ. Эти данные представлены как характеристика биосовместимости in vitro полимерных плёнок [6][7][8].…”

Section: изучение роста клеток In Vitro на подложках на основе пгбunclassified

Biocompatibility of electrospun poly(3-hydroxybutyrate) and its composites scaffoldsfor tissue engineering

et al. 2014

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Development of biodegradable polymers-based scaffolds for tissue engineering is a promising trend in bioengineering. The electrospun scaffolds from poly(3-hydroxybutyrate) (PHB) were produced using different additives that changed the physical and chemical characteristics of the products. As a result, the construct consisting of interwoven threads of different diameter (0.8-3.4 mm) were obtained, the smallest diameter was observed in the threads from the PHB using tetrabutilammonium iodide (TBAI) and titanium oxide II (TiO2) as additives. Mesenchymal stem cells (MSC) were cultivated on the scaffolds for the biocompatibility evaluation of obtained materials. Cells viability was determined by the XTT assay test. It was shown that the scaffold from the interwoven threads of lowest diameter is most favorable for MSC growth in comparison with the polymer film and scaffolds from the threads of larger diameter. Thus, it was shown that the biocompatibility of electrospun PHB scaffolds depended on their microstructure. The obtained data can be used for development of scaffolds for tissue engineering.

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The Terpolymer Produced by Azotobacter Chroococcum 7B: Effect of Surface Properties on Cell Attachment

Cited by 30 publications

References 43 publications

Poly(hydroxybutyrate‐co‐hydroxyvalerate) and bovine serum albumin blend prepared by electrospinning

Poly(hydroxybutyrate‐co‐hydroxyvalerate) and bovine serum albumin blend prepared by electrospinning

A study of synthesis and properties of poly‐3‐hydroxybutyrate/diethylene glycol copolymers

Biocompatibility of electrospun poly(3-hydroxybutyrate) and its composites scaffoldsfor tissue engineering

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