The Technology of Creating Restitution Sugar Beet Lines

Васильченко, Е Н; Zhuzhzhalova, T. P.; Vashchenko, Tatiana G.; Колесникова, Е О

doi:10.17238/issn2071-2243.2018.1.56

Cited by 3 publications

(2 citation statements)

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“…APM treatment yielded 64% genome doubling [103]. In other studies, treatment with different antimitotic compounds produced 2-4.7% doubling [79], 8.4% doubling [105], 19% [104], or doubling efficiency was not reported [16,74,83,102].…”

Section: Chemically Induced Haploid Genome Doubling In Vegetable Cropsmentioning

confidence: 93%

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Spontaneous and Chemically Induced Genome Doubling and Polyploidization in Vegetable Crops

Fomicheva,

Kulakov,

Alyokhina

et al. 2024

Horticulturae

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Plant ploidy manipulation is often required for breeding purposes. However, there is no comprehensive review covering genome doubling in vegetable crops despite the abundance of data for a large number of vegetable species. Similar to other species, genome doubling is required in vegetable crops to obtain doubled haploids (DHs). It is also utilized for the production of polyploids to overcome interspecific hybrid sterility and improve agricultural traits. Spontaneous haploid genome duplication (SHGD) occurs in many Apiaceae, Brassicaceae, Cucurbitaceae, and Solanaceae crops, allowing for the laborious treatment with antimitotic agents to be bypassed. SHGD mechanisms are not fully understood, but existing data suggest that SHGD can occur via nuclear fusion, endoreduplication, or other mechanisms during microspore or ovule early embryogenic development. Other studies show that SHGD can occur at later developmental stages during extended plant growth in vitro or ex vitro, possibly due to the presence of phytohormones in the medium and/or diploid cell competitive advantage. For unresponsive accessions and species with rare SHGD, such as onion (Allium cepa L.) and beet cultivars (Beta vulgaris subsp. vulgaris L.), antimitotic agent treatment has to be applied. Antimitotic agent application efficiency depends on the treatment conditions, especially the agent concentration and exposure time. Also, plant developmental stage is critical for agent accessibility and plant survival. The existing methods can be used to further improve genome doubling methodology for major vegetable crops and other species.

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Section: Chemically Induced Haploid Genome Doubling In Vegetable Cropsmentioning

confidence: 93%

“…vulgaris (var. saccharifera)) with 20 g/L colchicine, while Vasilchenko et al ( 2018) used it at a 4000 times smaller concentration (0.005 g/L) on the same crop [16,74]. Hence, the antimitotic agent should be tested in a wide range of concentrations to determine the most effective and cost-efficient treatment protocol.…”

Section: Antimitotic Agent Choicementioning

confidence: 99%

Spontaneous and Chemically Induced Genome Doubling and Polyploidization in Vegetable Crops

Fomicheva,

Kulakov,

Alyokhina

et al. 2024

Horticulturae

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Biotechnological methods as a tool for efficient sugar beet breeding

Zhuzhzhalova¹,

Колесникова²,

Васильченко³

et al. 2020

Vestn. VOGiS

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Here we consider aspects of the application of biotechnological methods to rapid creation, propagation, and maintenance of plants with improved or new traits in sugar beet breeding. The results of the works carried out in these fields by the Federal State Budgetary Scientific Institution “The A.L. Mazlumov All-Russia Research Institute of Sugar Beet” are reviewed. A close association between morphological and physiological changes in in vitro cultured organs and tissues, on the one hand, and breeding traits, on the other hand, which allows the development of experimental systems for non-amphimictic plant reconstruction is shown. The influence of in vitro growth conditions on haploid cells of unfertilized sugar beet ovules in the course of obtaining doubled haploid lines with high degree of homozygosity and maintenance of valuable breeding properties is considered. As compared to common inbreeding, this method shortens the time for development of homozygous material from 10–12 to 3–5 years, which is of great importance for speeding-up the breeding process. The results of studies on the culturing of mature sugar beet zygotic embryos based on in vitro selective systems have made it possible to improve the adaptive potential of plants and to provide complex resistance to environmental stress factors. Strict selection under abiotic stress conditions allowed creation of sugar beet isogenic lines with tolerance of drought, salinity, and soil acidity. It is shown that the proposed original design of mass-scale microclonal in vitro reproduction and deposition of elite plants as components of highly productive hybrids can be used to obtain seeds of uniform high-quality breeding material. The technologies developed by biotechnological methods are a topical and innovative direction of inquiry, since the application of these techniques to sugar beet breeding will promote obtaining of competitive hybrids with a set of commercially valuable traits. The combination of biotechnology methods, including tissue culture, and traditional breeding techniques is expected to provide an opportunity to obtain a new starting material to develop domestic varieties and hybrids of new generation with heterosis effect and a wide resistance spectrum persisting across generations.

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