The sugar substitute Stevia shortens the lifespan of Aedes aegypti potentially by N-linked protein glycosylation

Sharma, Arvind; Reyes, Jeremiah; Borgmeyer, David; Ayala-Chavez, Cuauhtemoc; Snow, Katie; Arshad, Fiza; Nuss, Andrew B.; Gulia-Nuss, Monika

doi:10.1038/s41598-020-63050-3

Cited by 15 publications

(30 citation statements)

References 33 publications

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“…When D. suzukii was fed erythritol, no erythrose was detected in the frass, suggesting that D. suzukii does not possess the metabolic pathway or associated enzymes to process erythritol (Tang et al, 2017). In the yellow fever mosquito, Aedes aegypti (L.), elevated expression of mannose-1-phosphate guanylytransferase was observed in erythritol-fed mosquitoes, suggesting that N-linked protein glycosylation could play a role in mortality, potentially through starvation of glucose (Sharma et al, 2020). Erythritol may competitively inhibit a-glucosidases in flies, as seen in humans (Wen et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Erythritol and xylitol are polyols that exist naturally at low quantities in some plants but are most often manufactured from plant tissues by microbial processing (Nigam & Singh, 1995;Moon et al, 2010). Recently, erythritol and xylitol have been assessed for their potential as alternative insect control compounds against a number of insect pests, including ants (Barrett et al, 2020), termites (Caponera et al, 2020), mosquitoes (Gilkey et al, 2018;Sharma et al, 2020), fruits flies (Baudier et al, 2014;O'Donnell et al, 2016;Choi et al, 2017;O'Donnell et al, 2017;Tang et al, 2017), and filth flies (Burgess & King, 2017;Fisher et al, 2017;Burgess & Geden, 2019). Erythritol and xylitol also have been suggested as possible replacements for sucrose in some types of house fly baits (Burgess et al, 2018;King et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Diets of erythritol, xylitol, and sucrose affect the digestive activity and gut bacterial community in adult house flies

Burgess

Taylor

Acevedo

et al. 2021

Entomologia Exp Applicata

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Polyol sweeteners, including erythritol and xylitol, have been investigated for their potential in insect control against a number of pests including mosquitoes, fruit flies, ants, and filth flies. Several insecticidal modes of action for the polyols have been proposed in non-biting flies and include: (1) dehydration from excessive regurgitation, (2) arrestment of physiological processes due to abnormally high hemolymph osmolality or osmotic diarrhea, and (3) alteration of the gut microbiome. Here, we explored the proposed modes of action in the house fly, Musca domestica L. (Diptera: Muscidae), a non-biting fly of medical and veterinary importance. House flies fed erythritol or xylitol regurgitated at a significantly higher rate when compared to flies that ingested sucrose. Flies that ingested sucrose solutions had higher hemolymph osmolality than erythritol but not xylitol, and deposited significantly more fecal spots than flies fed polyol solutions. Cultivable bacteria were least abundant in flies fed solutions of either sucrose or xylitol and most abundant in control flies provided water only. Of the potential modes of action tested, physiological stress from excessive post-feeding regurgitation seems the likeliest cause of death related to polyol consumption. However, we cannot rule out a classical toxicological or biochemical mode of action from these results. Although the efficacy of polyols as active ingredients is still under debate, their use as other components in fly control products may be beneficial. Due to widespread resistance to a limited number of insecticidal chemicals registered for house fly control, it is important that new control chemicals be thoroughly assessed for usefulness.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diets of erythritol, xylitol, and sucrose affect the digestive activity and gut bacterial community in adult house flies

Burgess

Taylor

Acevedo

et al. 2021

Entomologia Exp Applicata

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“…The adults were provided with water and a 10% sucrose solution ab libitum for regular colony maintenance. For egg production, adult females were maintained with an artificial membrane feeder on bovine blood [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization of Anopheles stephensi Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome

Speth

Kaur

Mazolewski

et al. 2021

Insects

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Several mosquito species within the genus Anopheles are vectors for human malaria, and the spread of this disease is driven by the propensity of certain species to feed preferentially on humans. The study of olfaction in mosquitoes is important to understand dynamics of host-seeking and host-selection; however, the majority of these studies focus on Anopheles gambiae or An. coluzzii, both vectors of malaria in Sub-Saharan Africa. Other malaria vectors may recognize different chemical cues from potential hosts; therefore, in this study, we investigated An. stephensi, the south Asian malaria mosquito. We specifically focused on the mouthparts (primarily the maxillary palp and labella) that have been much less investigated compared to the antennae but are also important for host-seeking. To provide a broad view of chemoreceptor expression, RNAseq was used to examine the transcriptomes from the mouthparts of host-seeking females, blood-fed females, and males. Notably, AsOr8 had a high transcript abundance in all transcriptomes and was, therefore, cloned and expressed in the Drosophila empty neuron system. This permitted characterization with a panel of odorants that were selected, in part, for their presence in the human odor profile. The responsiveness of AsOr8 to odorants was highly similar to An. gambiae Or8 (AgOr8), except for sulcatone, which was detected by AsOr8 but not AgOr8. Subtle differences in the receptor sensitivity to specific odorants may provide clues to species- or strain-specific approaches to host-seeking and host selection. Further exploration of the profile of An. stephensi chemosensory proteins may yield a better understanding of how different malaria vectors navigate host-finding and host-choice.

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“…The toxicity of erythritol was well documented for many other insect species, such as fruit fly [28], termites [29], house fly and stable fly [30,31], pear psylla [32]. mosquitoes [33], and pavement ant, Tetramorium immigrans Santschi [34].…”

Section: Naturally Occurring Compounds That Are Recently Evaluated As Potential Fire Ant Bait Active Ingredientsmentioning

confidence: 99%

Naturally Occurring Compounds/Materials as Alternatives to Synthetic Chemical Insecticides for Use in Fire Ant Management

Chen

2020

Insects

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The invasive red imported fire ant, Solenopsis invicta Buren (hereafter, fire ants), is a significant threat to public health and a danger to livestock, pets and wildlife due to their venomous stings. The fire ant has invaded many countries and regions and has become a globally significant pest. The current major tool to manage fire ants are synthetic insecticides that are used largely as stomach poisons in bait products or contact insecticides in spray, broadcast, drench, and dust products for area and nest treatments. In addition to these insecticide products, repellants and fumigants can also be useful in some unique scenarios. The ever-increasing public concern about the potential adverse effects of synthetic insecticides on health and the environment has been a driving force for searching for safer alternatives to control fire ants. Tremendous effort has been made in developing biologically-based control for managing fire ants; however, natural products continue to be one of the most attractive sources of safe alternatives to synthetic insecticides. Here, we summarized the synthetic insecticides that are currently used in managing fire ants, available alternative products in the current market, and academic efforts in searching for fire ant natural toxins, repellants and fumigants.

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The sugar substitute Stevia shortens the lifespan of Aedes aegypti potentially by N-linked protein glycosylation

Abstract: approach for mosquito control. Further understanding of the molecular mechanisms involved would help identify additional novel targets for vector control using ATSB.

Cited by 15 publications

References 33 publications

Diets of erythritol, xylitol, and sucrose affect the digestive activity and gut bacterial community in adult house flies

Diets of erythritol, xylitol, and sucrose affect the digestive activity and gut bacterial community in adult house flies

Characterization of Anopheles stephensi Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome

Naturally Occurring Compounds/Materials as Alternatives to Synthetic Chemical Insecticides for Use in Fire Ant Management

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