The study on interacting factors and functions of GASA6 in Jatropha curcas L.

Abstract

Background The gibberellic acid-stimulated Arabidopsis (GASA) gene encodes a class of cysteine-rich functional proteins and is ubiquitous in plants. Most GASA proteins are influence the signal transmission of plant hormones and regulate plant growth and development, however, their function in Jatropha curcas is still unknown. Results In this study, we cloned JcGASA6, a member of the GASA family, from J. curcas. The JcGASA6 protein has a GASA-conserved domain and is located in the tonoplast. The three-dimensional structure of the JcGASA6 protein is highly consistent with the antibacterial protein Snakin-1. Additionally, the results of the yeast one-hybrid (Y1H) assay showed that JcGASA6 was activated by JcERF1, JcPYL9, and JcFLX. The results of the Y2H assay showed that both JcCNR8 and JcSIZ1 could interact with JcGASA6 in the nucleus. The expression of JcGASA6 increased continuously during male flower development, and the overexpression of JcGASA6 was associated with filament elongation of the stamens in tobacco. Conclusion JcGASA6, a member of the GASA family in J. curcas, play an important role in growth regulation and floral development (especially in male flower). It is also involved in the signal transduction of hormones, such as ABA, ET, GA, BR, and SA. Also, JcGASA6 is a potential antimicrobial protein determined by its three-dimensional structure.

A New Advance on the Improvement of Forest Ecosystem Functions in the Karst Desertification Control

“…3.9. Focusing on the Layout of the Forest Wellness Industry for Desertification Control in the Context of the Era of Great Health, We Systematically Study the Extended Industrial Chain and Service Potential of Forest Ecosystems, and Identify the New Highlights of the Tourism Economy under the New Normal Select desertification areas with good ecological tourism infrastructure and location advantages fully utilize local forest wellness industry policies and layouts, combine their existing vegetation, and screen species that combine aspects of both ecology and economy [28][29][30] to study and construct an extended industrial chain of forest ecosystems from the perspectives of germplasm protection, research experience, wellness tourism, and medication and food therapy, in order to achieve an integrated leisure and health service system of forestry-tourism-rehabilitation, as well as the sustainable and healthy development of ecosystem restoration work in desertification areas.…”