The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25 S rRNA gene from Saccharomyces cerevisiae

Georgiev, O.I.; Nikolaev, N.; Hadjiolov, A.A.; Skryabin, K. G.; Zakharyev, V.M.; Bayev, A.A.

doi:10.1093/nar/9.24.6953

Cited by 251 publications

(103 citation statements)

References 23 publications

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“…The size of cytosol rRNAs in S . cerevisiae was identical to the published results (Georgiev et al, 1981;Rubtsov et al, 1980). Moreover, electrophoresis on glyoxal gels (Maniatis et al, 1982) also supported the above sizes (data not shown).…”

Section: Restriction Enzyme Analysissupporting

confidence: 90%

Characterization and Comparison of Mitochondrial DNAs and rRNAs from Penicillium Urticae and P. Chrysogenum

Sekiguchi¹,

Ohsaki²,

Yamamoto³

et al. 1990

Journal of General Microbiology

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Mitochondria1 DNA (mt DNA) from a patulin producer, Penicillium urticue (synonym P. griseofulvum), was 27.8kb+0.6kb in size by electron microscopy and 27.2kb by agarose gel electrophoresis. Restriction endonuclease maps for nine restriction enzymes were constructed, and eleven fragments which. covered the total range of themt DNA were cloned into the Escherichk coli plasmid vector pUCl9. Southern analysis of the native genomes of P. urtkue amlE chrysogenum with six of the cloned fragments as probes indicated similar genome arrangements as well as similar restriction mps.=.Both.the large and small rRNA genes of P. urticae and P. chrysogenum were located on these restriction maps using Southern hybridization,.and the result also supported the similar arrangement. Agaroselformaldehyde gel electrophoresis indicated that the small rRNA was 1.5 kb in size in both species; but, surprisingly, the large rRNA was 4.2 kb in size for P. d a e and 3.5 kb for P. chrysogenum.These sizes were, respectively, 1-1 kb and 0-4 kb larger than those from the very closely related Aspergillus nirlulans.

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Section: Restriction Enzyme Analysissupporting

confidence: 90%

Characterization and Comparison of Mitochondrial DNAs and rRNAs from Penicillium Urticae and P. Chrysogenum

Sekiguchi¹,

Ohsaki²,

Yamamoto³

et al. 1990

Journal of General Microbiology

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“…Yamadazyma philogaea CBS 6696 was linked to the cluster of Debaryomyces species at 8 base differences. Torulaspora globosa IFO 1160 and S cerevisiae IFO 2376 constituted a (4). N, A, G, C, or U.…”

Section: Resultsmentioning

confidence: 99%

“…The partial base sequences of 18S and 26S rRNAs of the yeast strains were determined by the method of Lane et al (10) using reverse transcriptase. The oligonucleotide DNA primers used in this experiment were 5'-ACGGGCGGTGTGTAC-3', which is complementary to the sequence of positions 1641 through 1627 of 18S rRNA (in Saccharomyces cerevisiae) (12) and 5'-GGTCCGTGTTTCAAGACGG-3' and 5'-TTGGAGACCTG-CTGCGG-3', which are complementary to the sequences of positions 654 through 636 and 1857 through 1841, respectively, of 26S rRNA (in S cerevisiae) (4).…”

Section: Methodsmentioning

confidence: 99%

The molecular phylogeny of the Q9-equipped ascomycetous teleomorphic yeast genus Debaryomyces Lodder et Kreger-van Rij based on the partial sequences of 18S and 26S ribosomal ribonucleic acids.

Yamada

Nagahama

Banno

1991

J. Gen. Appl. Microbiol.

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The partial base sequences of 18S and 26S rRNAs were examined in eighteen strains of Debaryomyces, Torulaspora, and Yamadazyma species including two strains of D. udenii. All of the strains of Debaryomyces species constituted a single group (cluster) phylogenetically. In the partial base sequence (positions 493 through 622, 130 bases) of 26S rRNA, the maximum homologies were 79-99% among Debaryomyces species. TT globosa and Y. philogaea had 71-78% and 81-87% maximum homologies, respectively, with Debaryomyces species. In the partial base sequence (positions 1611 through 1835, 225 bases) of 26S rRNA, the base differences numbered 5-0 among Debaryomyces species. T. globosa and YY philogaea had 15-13 and 10-8 base differences, respectively, with Debaryomyces species. In the partial base sequence (positions 1451 through 1618, 168 bases) of 18S rRNA, Debaryomyces species were divided into two subgroups (subclusters) .

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“…The partial base sequences of 18S and 26S rRNAs of these yeast strains were determined by the methods of Lane (13,14). The oligonucleotide DNA primers used in this experiment were 5'-ACGGGCGGTGTGTAC-3', which is complementary to the sequence of positions 1641 through 1627 of 18S rRNA (in Saccharomyces cerevisiae) (6) and 5'-GGTCCGTGTTTCAAGACGG-3' and 5'-TTGGAGACCTGCTGCGG-3', which are complementary to the sequences of positions 654 through 636 and 1857 through 1841, respectively, of 26S rRNA (in S. cerevisiae) (2).…”

Section: Methodsmentioning

confidence: 99%

The phylogenetic relationships of the q6-equipped generatorulaspora lindner and zygosaccharomyces barker (saccharomycetaceae) based on the partial sequences of 18s and 26s ribosomal ribonucleic acids.

Yamada¹,

Maeda²,

Nagahama³

et al. 1991

J. Gen. Appl. Microbiol.

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Eleven strains of Torulaspora and Zygosaccharomyces species were examined for the partial base sequences of 18S and 26S rRNAs. In the partial base sequences in positions 493-622 (130 bases) of 26S rRNA, there were 889l 0o and 70-92% maximum homologies within the genera Torulaspora and Zygosaccharomyces, respectively. The maximum homologies were 70-9090 between the two genera. The species of the two genera had 73-88% maximum homologies with Saccharomyces cerevisiae. In the partial base sequences in positions 1611-1835 (225 bases) of 26S rRNA, there were 3-0 and 16-1 base differences within the two genera, respectively. The base differences were 15-0 between the two genera. The two genera had 17-8 base differences with S. cerevisiae. In the partial base sequences in positions 1451-1618 (168 bases) of 18S rRNA, there were 0 and 3-0 base differences within the two genera, respectively. The base differences were 2-0 between the two genera. The two genera had 3-1 base differences with S cerevisiae. Zygosaccharomyces cidri and Z. fermentati occupied a unique situation (base differences, 3-2). The data

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The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25 S rRNA gene from Saccharomyces cerevisiae

Cited by 251 publications

References 23 publications

Characterization and Comparison of Mitochondrial DNAs and rRNAs from Penicillium Urticae and P. Chrysogenum

Characterization and Comparison of Mitochondrial DNAs and rRNAs from Penicillium Urticae and P. Chrysogenum

The molecular phylogeny of the Q9-equipped ascomycetous teleomorphic yeast genus Debaryomyces Lodder et Kreger-van Rij based on the partial sequences of 18S and 26S ribosomal ribonucleic acids.

The phylogenetic relationships of the q6-equipped generatorulaspora lindner and zygosaccharomyces barker (saccharomycetaceae) based on the partial sequences of 18s and 26s ribosomal ribonucleic acids.

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