The structure of pyoverdine Pa, the siderophore of Pseudomonas aeruginosa

Wendenbaum, S.; Demange, Pascal; Dell, Anne; Meyer, Jean‐Marie; Abdallah, Mohamed Abou-Elwafa

doi:10.1016/s0040-4039(00)94031-0

Cited by 147 publications

(109 citation statements)

References 6 publications

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“…This may go some way to explaining why P . aeruginosa retains two siderophore-mediated iron-uptake systems, despite the observation that the binding constant of iron for pyoverdin (Wendenbaum et al, 1983) is significantly greater than that of pyochelin (los at acid pH), which in addition requires two molecules to bind one molecule of iron (Cox & Graham, 1979). We speculate that P .…”

Section: Discussionmentioning

confidence: 99%

Siderophore-specific induction of iron uptake in Pseudomonas aeruginosa

Gensberg

Hughes²,

Smith³

1992

Journal of General Microbiology

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Pseudomonas aeruginosa has two siderophore-based high-affinity iron-uptake systems utilizing pyoverdin and pyochelin. Using strain IAl, a mutant deficient in production of both siderophores, we have shown that addition of purified siderophore to the growth medium induces expression of specific iron-regulated outer-membrane proteins and increases 55Fe-siderophore transport. Addition of pyoverdin from the parent strain P A 0 1 or from a clinical strain 0:12 induced expression of an 85 kDa IROMP and increased the rate of 55Fe-pyoverdin transport.Transport rates for 55Fe-PA01 pyoverdin increased from 1.27 to 3.57 pmol Fe min-l per lo9 cells. Addition of purified pyochelin induced expression of a 75 kDa IROMP accompanied with increased 55Fe-pyochelin uptake without affecting 55Fe-pyoverdin transport. 55Fe-pyochelin transport increased from 0.3 to 10.6 pmol min-' per lo9 cells. Addition of pyoverdin from the parent strain or a chromatographically distinct pyoverdin caused increased reactivity with an anti-85 kDa mAb in Western blotting, indicating that the same receptor is being induced. These results suggest that P. aeruginosa can respond specifically to the presence of siderophore and moreover that not only can the pyoverdin receptor transport its cognate ferri-pyoverdin but also different ferripyoverdms, albeit at a reduced rate.

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Section: Discussionmentioning

confidence: 99%

Siderophore-specific induction of iron uptake in Pseudomonas aeruginosa

Gensberg

Hughes²,

Smith³

1992

Journal of General Microbiology

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“…Hence, these biological recognition elements are suitable to develop novel, simple and selective fluorescence biosensors. Pyoverdine is a kind of extracellular siderophore of Pseudomonas aeruginosa, which can be largely secreted under iron-deficient conditions [19]. It helps Pseudomonas aeruginosa uptake iron to overcome iron limitation as well as protects them from heavy metal toxicity [20].…”

Section: Introductionmentioning

confidence: 99%

A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine

Yin

Wang

et al. 2016

Sensors and Actuators B: Chemical

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a b s t r a c tThe environmental copper pollution seriously threatens the health of organisms and the safety of ecosystem. Therefore, the development of a simple and sensitive method to detect copper ion is very important. In this study, we have developed a fluorescent biosensor based on biological recognition element pyoverdine to selectively detect copper ion. The fluorescence of pyoverdine is quenched obviously after binding with copper ion. A good linearity within the range of 0.2-10 M (R = 0.997) is attained and the detection limit is 50 nM. The biosensor has been successfully utilized for the detection of copper ion in drinking water, seawater and bio-samples and the results agree well with those obtained by the inductively coupled plasma mass spectrometry. Therefore, the established biosensor is a creditable method to detect copper ion with high sensitivity and selectivity, which can be utilized as a powerful tool to monitor copper pollution in the environment.

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“…We were interested to see whether this instability was specific for Tn5 or whether, as suggested by these authors, it is the result of an adaptation of P. aeruginosa to eliminate composite transposons from its genome. High affinity iron uptake in fluorescent pseudomonads is mediated by pyoverdine or pseudobactin, a complex fluorescent 0001-7231 0 1992 SGM P. Cornelis and others siderophore made of a catecholate chromophore and a peptide arm of variable length and composition containing unusual amino acids (Wendenbaum et al, 1983, Hohnadel & Meyer, 1988. Both catechol groups from the c hromophore and hydroxamate or hydroxycarboxylate groups provided by the unusual amino acids 6-Nhydroxyornithine or P-hydroxyaspartic acid respectively (depending on the pyoverdine) participate in complexing Fe(II1) (Hohnadel & Meyer, 1988).…”

Section: Introductionmentioning

confidence: 99%

Stability, frequency and multiplicity of transposon insertions in the pyoverdine region in the chromosomes of different fluorescent pseudomonads

Cornélis

Anjaiah

Koedam

et al. 1992

Journal of General Microbiology

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Tn5 mutagenesis of different fluorescent pseudomonads was achieved by conjugational transfer of the suicide vector @UP 10141. Pyoverdine negative (Pvd-) mutants were detected by the absence of fluorescence on King's B medium and by their inability to grow in the presence of the iron chelator EDDHA [ethylenediamine di(ohydroxyphenylacetic acid)j. In P.j?uorescens ATCC 17400 and three rhizosphere isolates (one P. putida and two P. fluorescens), the percentage of Pvd-mutants ranged between 0 and 0.54%. In a P. chlororuphis rhizosphere isolate, this percentage was higher (4 %). In these mutants both of the Tn5 antibiotic resistances (Km and Tc) were stable and the transposon could be detected by hybridization. In Pvd-mutants of P.j?uorescens ATCC 17400, the transposon was found to be inserted twice in the chromosome while single insertions were detected in the DNA of other, randomly tested mutants. In P. aeruginosa PAO1, where 13-1 % of the mutants were Pvd-, both antibiotic resistances were rapidly lost and accordingly no transposon insertion could be detected by hybridization. However, the Pvd-phenotype was generally stable in these mutants. The plasmid pNK862 containing a mini-TnlU transposon was introduced by electroporation into P. aeruginosa P A 0 1 and Kmr mutants were recovered, 89 % of which were Pvd-and confirmed to be P. aeruginosa by PCR amplification of the P. aeruginosa lipoprotein gene.The mini-Tnl0 insertions were also found to be unstable in PAO1.

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The structure of pyoverdine Pa, the siderophore of Pseudomonas aeruginosa

Cited by 147 publications

References 6 publications

Siderophore-specific induction of iron uptake in Pseudomonas aeruginosa

Siderophore-specific induction of iron uptake in Pseudomonas aeruginosa

A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine

Stability, frequency and multiplicity of transposon insertions in the pyoverdine region in the chromosomes of different fluorescent pseudomonads

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