The stratum corneum transcriptome in atopic dermatitis can be assessed by tape stripping

Sølberg, Julie; Jacobsen, Stine; Andersen, Jeppe Dyrberg; Litman, Thomas; Ulrich, Nina H.; Ahlström, Malin G.; Kampmann, Marie-Louise; Morling, Niels; Thyssen, Jacob P.; Johansen, Jeanne Duus

doi:10.1016/j.jdermsci.2020.10.011

Cited by 12 publications

(28 citation statements)

References 39 publications

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“…This could be due to the differences between gene expression of full biopsy samples vs stratum corneum tape samples where a major global difference is expected. 11 Six genes were differentially expressed between ACD and ICD.…”

Section: Discussionmentioning

confidence: 98%

“…In our study we did not rediscover transcriptomic differences between the biomarkers identified in this study (Data S4). This could be due to the differences between gene expression of full biopsy samples vs stratum corneum tape samples where a major global difference is expected 11 …”

Section: Discussionmentioning

confidence: 99%

“…From the controls, a healthy skin sample was taken from the hands (palmar or dorsal aspects). From each sample site, two consecutive standard D‐squame tape strips (Monaderm, Monaco, France) were collected applying uniform pressure as described previously 11,16 . For RNA extraction, both tapes were used.…”

Section: Methodsmentioning

confidence: 99%

“…Recent advances in the use of skin tape strips to obtain valid skin samples now enable research within immunotypes and specific biomarkers 9‐13 . In a previous study, we found that the stratum corneum transcriptome in AD could be assessed by tape stripping of the skin.…”

Section: Introductionmentioning

confidence: 94%

“…[9][10][11][12][13] In a previous study, we found that the stratum corneum transcriptome in AD could be assessed by tape stripping of the skin. 11 Based on a paired comparison with skin biopsies from the same patients, we identified established molecular markers of AD in the tape samples. In the current study, we are taking the next step by investigating if differences in the transcriptome can be detected by tape stripping of HE patients with different anatomical patterns, of different etiology, and main morphologies.…”

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confidence: 99%

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The transcriptome of hand eczema assessed by tape stripping

et al. 2021

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Background: No biomarkers have been identified that can classify subtypes of hand eczema (HE). Although skin biopsies represent the gold standard for investigations of the skin, the invasive technique is not favorable when investigating skin from sensitive areas. Recent advances in the use of skin-tape strips for molecular investigations enable noninvasive investigations of HE.Objective: By using whole transcriptome sequencing (WTS), the molecular profile of HE according to different localizations on the hands, etiologies, and clinical/ morphological subtypes was investigated.Methods: Thirty adult, Danish HE patients, 12 with and 18 without concurrent atopic dermatitis (AD), as well as 16 controls were included. Tape strip samples were collected from lesional, nonlesional, and healthy skin. Total RNA was extracted and WTS was performed. Results:The largest molecular difference of HE patients with and without AD was found in nonlesional skin areas and included a downregulation of CXCL8 for HE patients without AD. Differences between allergic and irritant contact dermatitis included epidermal biomarkers such as EPHA1. Conclusion:Skin tape strip samples could be used to assess the gene expression profile of HE on different localizations of the hands. The skin tape strip method identified new molecular markers that showed promising result for the identification of HE subtypes.atopic hand eczema, contact dermatitis, subtypes of hand eczema, tape stripping, transcriptomics | INTRODUCTIONHand eczema (HE) is a prevalent disease with a 1-year prevalence of 9% in the general population. 1 It may affect quality of life, impact work ability, 2,3 and require treatment periodically or continuously, depending on severity and chronicity. 4 HE may be a result of different etiologies, 5 which complicates both effective treatment and prevention. HE can be a part of atopic dermatitis (AD), and/or environmental factors such as exposure to allergens and/or irritants may result in allergic contact dermatitis (ACD) and/or irritant contact dermatitis (ICD) on the hands. The skin impairment of AD also makes the skin more vulnerable to irritants, sometimes leading to a mixed pattern of AD and ICD.Abbreviations: ACD, allergic contact dermatitis; AD, atopic dermatitis; DEG, differentially expressed gene; GO, gene ontology; HECSI, hand eczema severity index; HE +AD , hand eczema with atopic dermatitis; HE -AD , hand eczema without atopic dermatitis; HE, hand eczema; ICD, irritant contact dermatitis; WTS, whole transcriptome sequencing.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

mentioning

confidence: 99%

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