The Standardization of Fecal Smears for Estimating Egg Production and Worm Burden

Beaver, Paul C.

doi:10.2307/3273171

Cited by 47 publications

(14 citation statements)

References 3 publications

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“…Stool samples have been collected for three consecutive days, and a single thick smear, using the Hein protocol (Beaver, 1950), was prepared from each stool sample. Positive samples from 46 children (age: 9 months to 16 years) with confirmed ascariasis and/or trichuriasis were re-examined using the quantitative coprological McMaster technique (Coles et al, 1992).…”

Section: Methodsmentioning

confidence: 99%

Nematode infections in Slovak children hospitalised during 2008–2009

et al. 2010

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A study involved 1800 hospitalised children (age: 9 months to 16 years) examined by ovoscopic analyses and confirmed the occurrence of Ascaris lumbricoides and Trichuris trichiura in 46 patients (2.55 %). Of these, 30 patients had Ascaris infection, 13 were positive for both nematodes and 3 patients had Trichuris infection. The mean count of A. lumbricoides eggs in positive cases was 1050 eggs per gramme (EPG) in range 150 -4450 EPG. The mean count of T. trichiura eggs was 150 EPG (50 -250 EPG). The highest intensities of A. lumbricoides and T. trichiura infections occurred in children 3 -5 years of age living in poor hygienic conditions. Most common clinical conditions in all the patients included anaemia combined with complicated bronchopneumonia, colitis and gastritis. The strongest correlation between the parasite burden and selected laboratory test data (eosinophil count, haemoglobin, total serum iron) was found in children of 2 years of age (P < 0.05) and decreased with age.

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Section: Methodsmentioning

confidence: 99%

Nematode infections in Slovak children hospitalised during 2008–2009

et al. 2010

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“…Formalin-preserved stool samples and the modified Ziehl-Neelsen carbol-fuchsin staining of formalin-ether concentrates were used for the recovery and identification of C. parvum oocysts. 21 Helminth ova counts were made using the standard smear egg count technique, 22 and the results were expressed as eggs per gram feces (epg). The helminth intensity was classified as follows: Ascaris lumbricoides, Ͻ 10,000 to Ͼ 20,000 epg; Trichuris trichiura, Ͻ 5,000 to Ͼ 10,000 epg, and hookworm Ͻ 2,000 to Ͼ 5,000 epg were considered light, moderate, and heavy infections, respectively.…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%

Intestinal parasitic infections, with a special emphasis on cryptosporidiosis, in Amerindians from western Venezuela.

Chacín-Bonilla

Sanchez-Chavez²

2000

The American Journal of Tropical Medicine and Hygiene

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Abstract. The prevalences of intestinal parasites and intensities of helminth infections were studied in two Amerindian villages in Venezuela. Single stool specimens were collected from 303 individuals from Saimadoyi and 130 from Campo Rosario. Wet mounts, iron-hematoxylin-stained smears, and formalin-ether concentrates were examined for the presence of parasites; modified Ziehl-Neelsen carbol-fuchsin staining of 10% formalin-preserved stool was used to identify Cryptosporidium parvum. Helminth ova counts were made using the standard smear egg count technique. Mixed infections (Campo Rosario ϭ 69.9%, Saimadoyi ϭ 71.6%) were frequent. Overall infection rates with one or more species (Campo Rosario ϭ 79.2%, Saimadoyi ϭ 95.4%; P Ͻ 0.01) and with any protozoans (Campo Rosario ϭ 60.8%, Saimadoyi ϭ 72.3%; P Ͻ 0.05) were high and predominant (P Ͻ 0.05) in Saimadoyi. Cryptosporidiosis was identified in 38 subjects (8.8%) in both villages; 60.6% were asymptomatic carriers. The mean egg counts of helminths were heavier in Campo Rosario (P Ͻ 0.05), which was probably due to the drastic reduction of their lands along with their low standard of living. This study documents the change of intestinal parasitism pattern and deterioration of the health of Amerindians by the process of acculturation.

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“…Quantitative egg counts were carried out among positive cases using Beaver' s technique (Beaver, 1950) and the number of eggs/g stool estimated from the mean of three measurements. Hookworm larvae were identi® ed to species as described by Harada and Mori (1955).…”

Section: Methodsmentioning

confidence: 99%

Parasitic infections in villagers from districts of the Brazilian three Amazon

Thatcher¹

1998

Annals of Tropical Medicine And Parasitology

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A survey of intestinal parasites among 827 rural villagers in the Brazilian Amazon revealed that the prevalences of infection with Necator americanus, Trichuris trichiura and/or Ascaris lumbricoides were high. However, the intensities of infection with the two parasites for which there were enough data on intensity for further analysis, N. americanus and T. trichiura, were only low or moderate in each of the three districts studied. Possible reasons for the unusual (but not unique) absence of high intensities include moderately good sanitation, low population densities, the way in which work activities are located geographically, the use of indigenous anthelmintics and the geographical isolation of the communities which provided the subjects. Whether the light to moderate levels of infection observed require intervention is discussed.

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The Standardization of Fecal Smears for Estimating Egg Production and Worm Burden

Cited by 47 publications

References 3 publications

Nematode infections in Slovak children hospitalised during 2008–2009

Nematode infections in Slovak children hospitalised during 2008–2009

Intestinal parasitic infections, with a special emphasis on cryptosporidiosis, in Amerindians from western Venezuela.

Parasitic infections in villagers from districts of the Brazilian three Amazon

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