The stability of DNA–porphyrin complexes in the presence of Mn(II) ions

Ananyan, Gayane V.; Avetisyan, A.; Aloyan, L. R.; Dalyan, Yeva B.

doi:10.1016/j.bpc.2011.03.002

Cited by 15 publications

(6 citation statements)

References 30 publications

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“…24 On incubating the formed DNA biosensor with either the individual hemin or the target, no obvious change in ECL signal could be observed (data not shown), indicating that the short target DNA strand could not open the MB to form the hemin/G-quadruplex structure. 25 On the contrary, the ECL response dropped to its original 7.5% aer simultaneous incubation with both hemin and the target DNA (Fig. 4A, curve e), which validated that the hairpin could be dissociated aer the MB hybridized with the target DNA upon the assistance of hemin to form hemin/Gquadruplex architecture, and the formed architecture could quench the QDs-based ECL emission.…”

Section: Ecl Behaviors Of Qds/hemin/g-quadruplex Architecturementioning

confidence: 75%

Label-free electrochemiluminescent detection of DNA by hybridization with a molecular beacon to form hemin/G-quadruplex architecture for signal inhibition

et al. 2013

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A facile label-free electrochemiluminescent (ECL) DNA sensor was designed using a molecular beacon with a guanine-rich stem as a recognition probe. The ECL emission was produced from surface unpassivated CdTe quantum dots (QDs) co-immobilized with colloidal gold nanoparticles (AuNPs) on a chitosan-modified electrode surface. The molecular beacon was adsorbed onto the AuNPs by the thiolated stem. Upon the hybridization of the molecular beacon with target DNA to open the cycle in the presence of hemin, the dissociated guanine-rich sequence could conjugate hemin to form a G-quadruplex architecture. The formed DNAzyme then catalyzed the reduction of dissolved oxygen, the endogenous coreactant for ECL emission of QDs, leading to a decrease in ECL signal. The variations in surface morphology during the fabrication and recognition processes of the ECL sensor were characterized by atomic force microscopy and electrochemical impedance spectroscopy. The ECL signal inhibition depended linearly on the logarithmic value of DNA concentration ranging from 5.0 fM to 0.1 nM, with a detection limit of 0.9 fM. This proposed label-free method is a promising application of QDs-based ECL emission for ultrasensitive DNA assay.

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Section: Ecl Behaviors Of Qds/hemin/g-quadruplex Architecturementioning

confidence: 75%

Label-free electrochemiluminescent detection of DNA by hybridization with a molecular beacon to form hemin/G-quadruplex architecture for signal inhibition

et al. 2013

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“…Mn porphyrin has been a key compound in PTT due to its high photothermal conversion ability and good MR imaging contrast. 42,43 Zhang et al have designed an Mn porphyrin MOF, in which Zr 4+ and Mn porphyrin self-assemble. 44 MOFs have been used to transport gases such as NO due to their excellent porosity.…”

Section: Applications Of Metalloporphyrin Compounds In Pttmentioning

confidence: 99%

Application of metalloporphyrin sensitizers for the treatment or diagnosis of tumors

Luo

Wang

Chen

et al. 2022

Journal of Chemical Research

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At present, metalloporphyrin compounds demonstrate three main uses as anticancer sensitizers: (1) photosensitizers, (2) photothermal conversion agents, and (3) ultrasound sensitizers. Developing efficient sensitizers for cancer with excellent controllability and biocompatibility is an important goal of oncology medicine. Because of the different structural diversity of anticancer sensitizers, such sensitizers are used for treating cancers by employing a variety of tumor treatment methods such as mature photodynamic therapy, commonly used clinically photothermal therapy and promising sonodynamic therapy. Among the many sensitizers, metalloporphyrin-complex sensitizers attract wide attention due to their excellent performance in tumor treatment and diagnosis. This review briefly describes some metalloporphyrin anticancer drugs and diagnostic agents related to photodynamic, photothermal and sonodynamic therapy, and discusses the roles of metal atoms in these drugs.

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“…The pyridyl groups of MTP have the potential to base stack and/or form hydrogen bonds with nucleobases of DNA at physiological pH and the phosphodiester backbone of DNA may confer aqueous solubility to the complex. We demonstrate that unlike previous porphyrin:DNA complexes, 14–18 the PDN developed with this approach form discrete porphyrin:DNA nanoparticles that are of the appropriate size for drug-delivery applications via the enhanced permeability and retention (EPR) effect 19,20 and that readily dissociate upon cell internalization to deliver a therapeutic payload.…”

Section: Introductionmentioning

confidence: 95%

Non-covalent assembly of meso-tetra-4-pyridyl porphine with single-stranded DNA to form nano-sized complexes with hydrophobicity-dependent DNA release and anti-tumor activity

Ghosh

Üçer

D’Agostino

et al. 2014

Nanomedicine: Nanotechnology, Biology and Medicine

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DNA and porphyrin based therapeutics are important for anti-cancer treatment. The present studies demonstrate single-stranded DNA (ssDNA) assembles with meso-tetra-4-pyridyl porphine (MTP) forming porphyrin:DNA nano-complexes (PDN) that are stable in aqueous solution under physiologically relevant conditions and undergo dissociation with DNA release in hydrophobic environments, including cell membranes. PDN formation is DNA-dependent with the ratio of porphyrin:DNA being approximately two DNA nucleobases per porphyrin. PDN produce reactive oxygen species (ROS) in a light-dependent manner under conditions that favor nano-complex dissociation in the presence of hydrophobic solvents. PDN induce light-dependent cytotoxicity in vitro and anti-tumor activity towards bladder cancer xenografts in vivo. Light-dependent, PDN-mediated cell death results from ROS-mediated localized membrane damage due to lipid peroxidation with mass spectrometry indicating the generation of the lipid peroxidation products 9- and 13-hydroxy octadecanoic acid. Our results demonstrate that PDN have properties useful for therapeutic applications, including cancer treatment.

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The stability of DNA–porphyrin complexes in the presence of Mn(II) ions

Cited by 15 publications

References 30 publications

Label-free electrochemiluminescent detection of DNA by hybridization with a molecular beacon to form hemin/G-quadruplex architecture for signal inhibition

Label-free electrochemiluminescent detection of DNA by hybridization with a molecular beacon to form hemin/G-quadruplex architecture for signal inhibition

Application of metalloporphyrin sensitizers for the treatment or diagnosis of tumors

Non-covalent assembly of meso-tetra-4-pyridyl porphine with single-stranded DNA to form nano-sized complexes with hydrophobicity-dependent DNA release and anti-tumor activity

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