The sphingolipid composition of bovine kidney cortex, medulla and papilla

Karlsson, Karl‐Anders; Samuelsson, Bo E.; Steen, Göran O.

doi:10.1016/0005-2760(73)90072-6

Cited by 129 publications

(30 citation statements)

References 62 publications

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“…and one day with chloroform-methanol 1:9 (by vol.). The combined extracts were evaporated and subjected to mild alkaline degradation, dialysis, DEAE and silicic acid chromatography mainly as described [4]. To free the non-acid glycolipid fraction of alkali-stable phospholipids, acetylation and silicic acid chromatography were used [S].…”

Section: Methodsmentioning

confidence: 99%

Human blood group a‐positive and ‐negative strains of rat. Chemical basis as shown by fucolipids of small intestine

Breimer¹,

Hansson²,

Karlsson³

et al. 1980

FEBS Letters

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Section: Methodsmentioning

confidence: 99%

Human blood group a‐positive and ‐negative strains of rat. Chemical basis as shown by fucolipids of small intestine

Breimer¹,

Hansson²,

Karlsson³

et al. 1980

FEBS Letters

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“…The total non-acid glycolipid fraction was prepared by conventional steps like mild alkaline degradation, silitic acid and DEAE-cellulose chromatography [4], and a combinatory use of native and acetylated [5] substances. Methylation and reduction was done as in [6].…”

Section: Methodsmentioning

confidence: 99%

“…A detailed description of mass scale cali- Figure 1 shows a thin-layer chromatogram of the total non-acid glycolipids of an individual cat small intestine (A) and, for comparison, corresponding fractions of other species (B-H) to be discussed elsewhere. All spots were coloured green by the anisaldehyde reagent and therefore contained sugar [4]. The number of sugars of the glycolipids is indicated in the margin.…”

Section: Methodsmentioning

confidence: 99%

Structure determination of blood group type glycolipids of cat small intestine by mass fragmentography

et al. 1978

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“…Glycoli pids separated on TLC plates (Merck. Darmstadt, Germany) were ana lyzed by staining with an anisaldéhyde reagent [17]. Glycolipids sep arated on TLC plates (Whatman Ltd., UK) were labelled with antibod ies in the chromatogram binding assay (CBA) using a modification of the method of Magnani et al [18] and Hansson et al [19], All assays were performed in duplicate on separate occasions with identical re sults being obtained.…”

Section: Glycolipid Analysismentioning

confidence: 99%

Detection and Characterization of Lewis Antigens in Plasma of Lewis-Negative Individuals

Henry¹,

Oriol²,

Samuelsson³

1994

Vox Sanguinis

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Nonacid plasma glycolipids from Lewis-negative individuals of nonsecretor, partial-secretor and secretor phenotypes were prepared and separated by thin- layer chromatography and immunostained with radiolabelled Lewis antibodies. Lewis-positive plasma and intestinal epithelial cell glycolipids from Caucasians representing the four recognized Lewis and secretor combined phenotypes were used as controls. By presenting these purified total glycolipids in a cell-free environment to Lewis antibodies we were able to demonstrate the presence of small amounts of Lewis antigens in Lewis-negative individuals. It is shown that lacto- tetraosylceramide and extended precursor glycolipids are present in all Le(a-b -) nonsecretors. Le^b was detected in 1 of the 3 Le(a-b-) nonsecretor plasmas and in the intestinal sample of the same phenotype. Lactotetraosylceramide was absent but H type 1 and Leb were both present in all group O Le(a-b-) secretors, and extended H typel reactive structures were also found in the partial secretor. These results clearly demonstrate that although the Lewis-negative phenotype exists at the serological level, this phenotype is not an ‘all-or-nothing’ phenomenon at the chemical level. We also show that in the presence of reduced fucosyltransferase activity, increased elongation of the precursor chain occurs, which allows us to postulate that fucosylation of the precursor prevents or at least markedly reduces chain elongation.

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The sphingolipid composition of bovine kidney cortex, medulla and papilla

Cited by 129 publications

References 62 publications

Human blood group a‐positive and ‐negative strains of rat. Chemical basis as shown by fucolipids of small intestine

Human blood group a‐positive and ‐negative strains of rat. Chemical basis as shown by fucolipids of small intestine

Structure determination of blood group type glycolipids of cat small intestine by mass fragmentography

Detection and Characterization of Lewis Antigens in Plasma of Lewis-Negative Individuals

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