The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme

Abstract

None.

The impact of sperm protamine deficiency and sperm DNA damage on human male fertility: a systematic review and meta-analysis

“…Indeed, 87% of patients with abnormally high P1/P2 ratios exhibited P2 under-expression, while only a small proportion of patients with high P1/P2 exhibited P1 over-expression (13%) (Aoki et al, 2005a). Moreover, in the subfertile patients, highly aberrant protamine ratio was much more common than lowly aberrant protamine ratio, with the ratio of 2.92 (108/37) (Aoki et al, 2005a), 1.86 (26/14) (Aoki et al, 2006a), and5.91 (65/11) (de Mateo et al, 2009) reported by three of the studies included in our review, which was consistent with the results of own cohort studies (Rogenhofer et al, 2013). Therefore, in this meta-analysis, the overall protamine ratio in the infertile patients was much higher than in the controls, although there was a small part of patients with low protamine ratios.…”

“…As the raw data in one article were extracted from a multicenter study (Rogenhofer et al, 2013), the results of protamine-mRNA ratios of the different study centers were analyzed in this systematic review in order to prevent measurement bias. We subanalyzed the data with regard to the different types of assays used to Figure 3 Funnel plot of the included studies used to evaluate the correlation coefficient (COR) between protamine deficiency and sperm DNA damage.…”

“…A total of nine studies with 633 patients and 463 controls in the first part, which fulfilled all of the inclusion criteria, were considered for the analysis. Five of the studies were prospective (de Mateo et al, 2009;Garcia-Peiro et al, 2011;Rogenhofer et al, 2013;Ni et al, 2014;Savadi-Shiraz et al, 2015) and four were retrospective (Mengual et al, 2003;Aoki et al, 2005aAoki et al, , 2006aSteger et al, 2008). Four studies used real-time RT-qPCR (mRNA) (Steger et al, 2008;Rogenhofer et al, 2013;Ni et al, 2014;Savadi-Shiraz et al, 2015) and five studies employed PAGE (protein) (Mengual et al, 2003;Aoki et al, 2005aAoki et al, , 2006ade Mateo et al, 2009;Garcia-Peiro et al, 2011) to calculate the protamine ratio.…”

“…Four studies used real-time RT-qPCR (mRNA) (Steger et al, 2008;Rogenhofer et al, 2013;Ni et al, 2014;Savadi-Shiraz et al, 2015) and five studies employed PAGE (protein) (Mengual et al, 2003;Aoki et al, 2005aAoki et al, , 2006ade Mateo et al, 2009;Garcia-Peiro et al, 2011) to calculate the protamine ratio. When comparing the differences in the protamine ratio between experimental and control groups, two studies compared normozoospermic healthy donors with subfertile patients (Steger et al, 2008;Savadi-Shiraz et al, 2015), five studies compared proven fertile donors with subfertile patients (Mengual et al, 2003;Aoki et al, 2005aAoki et al, , 2006ade Mateo et al, 2009;Garcia-Peiro et al, 2011), one study compared groups of men contributing to successful fertilization with those involved in fertilization failure after IVF and ICSI treatment (Rogenhofer et al, 2013), and one study compared spontaneous pregnant couples with non-pregnant couples (Ni et al, 2014). In the second part, the total number of studies included in the review was 12 comprising of 845 participants.…”

“…It is well-known that various mammalian species exhibit a species-specific protamine ratio (Corzett et al, 2002;Gosalvez et al, 2011) and that abnormal protamine ratios are associated with male subfertility (Rogenhofer et al, 2013;Francis et al, 2014) as well as reduced embryo quality Depa-Martynow et al, 2012). Aberrant protamine ratios have, in addition, been linked with increased levels of DNA damage in spermatozoa of mouse (Cho et al, 2003), man (Aoki et al, 2005b;Steger et al, 2008;Simon et al, 2011), bull (Fortes et al, 2014), and stallion (Paradowska-Dogan et al, 2014).…”