The specific binding of Listeria monocytogenes-immune T lymphocytes to macrophages. I. Quantitation and role of H-2 gene products.

Ziegler, K; Unanue, Emil R.

doi:10.1084/jem.150.5.1143

Cited by 58 publications

(32 citation statements)

References 26 publications

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“…It is reasonable to speculate, therefore, that if carbohydrate-containing antigens interact with macrophage receptors, they may alter macrophage-T cell interaction and hence immune responses (46).…”

Section: Resultsmentioning

confidence: 99%

Influence of antigen on immune complex behavior in mice.

et al. 1981

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A B S T R A C T To explore the possibility that the behavior of immune complexes can, under some circumstances, be directed by the antigen, we have studied the behavior of complexes of identical size made with the glycoproteins, orosomucoid (OR), and ceruloplasmin; or with their desialylated derivatives, asialo-orosomucoid (ASOR) and asialo-ceruloplasmin. Such desialylated proteins are rapidly removed from the circulation by a hepatic cell receptor for galactose, the sugar exposed upon removal of sialic acid.Mixtures of 1251-goat anti-ASOR with either ASOR or OR and mixtures of 125 I-rabbit anti-OR with either ASOR or OR form complexes identically. The complexes were separated by density gradient centrifugation and injected intravenously into C3H mice. Blood clearances and hepatic uptake of the OR complexes and ASOR complexes were markedly different. T1/2 for the goat OR complexes exceeded 300 min, whereas that for the ASOR complexes was 15 min. More detailed studies using rabbit complexes of various sizes revealed that light rabbit complexes behaved similarly to the goat complexes. The light rabbit OR complexes were cleared slowly, with only 18% found in the liver at 60 min, whereas the light rabbit ASOR complexes were cleared much more rapidly, with 62% found within the liver by 30 min. This rapid clearance was completely suppressed by a prior injection of a blocking dose ofASOR, which implies uptake by a galactosemediated mechanism on hepatocytes. As plexes were cleared more rapidly than light OR complexes but not so rapidly as heavy ASOR complexes. The clearance and hepatic uptake of the heavy OR complexes were markedly suppressed by a prior injection of heat-aggregated gamma globulin, a known Fc receptor-blocking agent (45% hepatic uptake without and 6% with aggregated gamma globulin). The heavy rabbit ASOR complexes exhibited inhibition of blood clearance and hepatic uptake by both galactose receptor-blocking and Fc receptor-blocking agents. A blocking dose of ASOR reduced the hepatic uptake at 30 min from 75 to 49%, and heat-aggregated gamma globulin reduced it from 75 to 39%, which suggests that these heavy complexes were removed from the circulation by receptors both for the immunoglobulin and for the antigen. Cell separation studies and autoradiographs confirmed that those complexes cleared primarily by galactose-mediated mechanism were within hepatocytes, and those cleared by Fc receptors were within the nonparenchymal cells of the liver. It seems probable, therefore, that some antigen-antibody complexes may be removed from the circulation via receptors not only for immunoglobulin but also for antigen.

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Section: Resultsmentioning

confidence: 99%

Influence of antigen on immune complex behavior in mice.

et al. 1981

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“…However, treatment of Δspa-infected mice with nSpA caused enhanced SpA KK + (V H 3 + ) IgM and IgG antibody production as early as day 12 post infection (Fig. 4 F and G) Antigen recognized by B-cell receptors is endocytosed, processed, and loaded onto MHC class II (H-2) compartments for presentation to CD4 + T cells (37). The quality and quantity of antibody responses to a given antigen are under genetic control of the H-2 locus (38).…”

Section: Igbd Repeat Variation Affects Staphylococcal Binding To Ig Hmentioning

confidence: 99%

“…1 B and D). These data indicate that protein A, specifically the D 36 , 37 residues in each of the five IgBDs responsible for heavy-chain binding to B-cell receptors, is required for the expansion of V H 3 clonal antibodies during S. aureus infection. (28), whose coding sequence is located upstream of the highly variable region X in the spa gene, otherwise used for genotyping S. aureus (27,32,33) (Fig.…”

mentioning

confidence: 99%

“…A variant, spa KKAA , with substitutions of these glutaminyl (Q 9 K, Q 10 K) and aspartyl (D 36 A, D 37 A) residues in each of the five IgBDs, is unable to bind the V H 3 variant chains or Fcγ of immunoglobulins (24). In contrast, spa variants carrying only glutaminyl (Q 9 K, Q 10 K in SpA KK ) or aspartyl substitutions (D 36 A, D 37 A in SpA AA ) in their five IgBDs retain the ability to bind either V H 3 variant chains or Fcγ, respectively (24). When used in the i.v.…”

mentioning

confidence: 99%

“…Each IgBD is endowed with two conserved glutaminyl (Q 9,10 ) and aspartyl residues (D 36,37 ) that are critical for Fcγ and Fab binding, respectively (25). A variant, spa KKAA , with substitutions of these glutaminyl (Q 9 K, Q 10 K) and aspartyl (D 36 A, D 37 A) residues in each of the five IgBDs, is unable to bind the V H 3 variant chains or Fcγ of immunoglobulins (24).…”

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confidence: 99%

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Peptidoglycan-linked protein A promotes T cell-dependent antibody expansion duringStaphylococcus aureusinfection

Kim

Falugi

Missiakas

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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A hallmark of Staphylococcus aureus disease in humans is persistent infections without development of protective immune responses. Infected patients generate V H 3 plasmablast expansions and increased V H 3 idiotype Ig; however, the mechanisms for staphylococcal modification of immune responses are not known. We report here that S. aureus-infected mice generate V H 3 antibody expansions via a mechanism requiring MHC-restricted antigen presentation to CD4 + T cells and staphylococcal protein A (SpA), a cell wall-anchored surface molecule that binds Fcγ and V H 3 variant heavy chains of Ig. V H 3 expansion occurred with peptidoglycan-linked SpA from the bacterial envelope but not with recombinant SpA, and optimally required five tandem repeats of its Ig-binding domains. Signaling via receptor-interacting serine/threonine protein kinase 2 (RIPK2) was essential for implementing peptidoglycan-linked SpA superantigen activity. V H 3 clan IgG from S. aureus-infected or SpA-treated animals was not pathogen-specific, suggesting that SpA cross-linking of V H 3 idiotype B-cell receptors and activation via attached peptidoglycan are the determinants of staphylococcal escape from adaptive immune responses.

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Antigen processing and presentation by macrophages

Allen

Unanue

1984

Am. J. Anat.

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The classical macrophage is one of the most important cells involved in presenting antigen to helper T cells, because of its ability to regulate its expression of Ia molecules and to encounter and process particulate and soluble antigens. We have summarized in this report studies examining the handling by macrophages of two different antigens, the bacteria Listeria monocytogenes and the protein hen egg white lysozyme (HEL). The purpose was to identify potential sources of immunogenic peptides. Presentation of Listeria required an intracellular processing stage sensitive to lysosomotropic drugs. The Listeria required internalization and processing, after which immunogenic molecules were recognized by T cells on the macrophage surface. Metabolic studies showed that Listeria-derived peptides were released by macrophages that had phagocytosized the bacteria. The release of these peptides was a temperature-dependent process, unaffected by inhibiting lysosomal catabolism by treatment with chloroquine. Listeria-derived peptides were also detected on the surface of the macrophage. These peptides behaved like integral membrane proteins, some of which persisted for at least 24 hr at the macrophage surface. When tested for immunogenicity, the released peptides were very weakly immunogenic. The membrane-associated peptides alone could not stimulate Listeria-specific T cells, but could be reprocessed by additional macrophages and subsequently stimulate the T cells. A defined antigen system using HEL-specific T-cell hybridomas was used to examine the processing of HEL. Presentation of HEL required a chloroquine-sensitive intracellular processing stage. In examining two T-cell hybridomas, a differential requirement for antigen processing was determined.(ABSTRACT TRUNCATED AT 250 WORDS)

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The specific binding of Listeria monocytogenes-immune T lymphocytes to macrophages. I. Quantitation and role of H-2 gene products.

Cited by 58 publications

References 26 publications

Influence of antigen on immune complex behavior in mice.

Influence of antigen on immune complex behavior in mice.

Peptidoglycan-linked protein A promotes T cell-dependent antibody expansion duringStaphylococcus aureusinfection

Antigen processing and presentation by macrophages

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