1992
DOI: 10.1080/02681219280000331
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Abstract: We studied the influence of the growth factor (GF) source, concentration and production time on the plating efficiency of Paracoccidioides brasiliensis yeast cells. The highest plating efficiencies were achieved when the GF was derived from a fast growing P. brasiliensis isolate which was not homologous to the plated samples.The colony forming units (c.f.u.) procedure is widely used when quantitation of viable micro-organisms is desired such as in studies concerning killing of bacteria or fungi by specific and… Show more

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Cited by 102 publications
(64 citation statements)
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“…Single cells (clones) were obtained in both experiments (i and ii), forming colonies only in supplemented BHI agar, as also reported by others (10,12). The colonies were then transferred to a new petri dish containing supplemented BHI, and after 10 days they measured 1 cm in diameter.…”
mentioning
confidence: 80%
“…Single cells (clones) were obtained in both experiments (i and ii), forming colonies only in supplemented BHI agar, as also reported by others (10,12). The colonies were then transferred to a new petri dish containing supplemented BHI, and after 10 days they measured 1 cm in diameter.…”
mentioning
confidence: 80%
“…Animals from each group were sacrificed and the enumeration of viable organisms was done as previously described (29). The numbers (log 10 ) of viable P. brasiliensis per g of tissue (in vivo) or per ml of cell homogenates (in vitro) are expressed as the means Ϯ SEs.…”
Section: Cfu Assays Mortality Rates and Histologic Analysis To Detementioning
confidence: 99%
“…Six to eight animals from each group were sacrificed at 4 wk p.i., and the enumeration of viable organisms in the three organs was performed as previously described (20). Briefly, aliquots (100 l) of the cellular suspension of each organ were plated on brain-heart infusion agar (Difco, Detroit, MI) supplemented with 4% (v/v) horse serum (Instituto Butantan, Sao Paulo, Brazil) and 5% Pb192 culture filtrate, the latter constituting a source of growth-promoting factor.…”
Section: Cfu Assaymentioning
confidence: 99%