The Sonic Hedgehog–Patched–Gli Signaling Pathway Maintains Dental Epithelial and Pulp Stem/Progenitor Cells and Regulates the Function of Odontoblasts

Ishikawa, Yuko; Ida‐Yonemochi, Hiroko; Saito, Kotaro; Nakatomi, Mitsushiro; Ohshima, Hayato

doi:10.3389/fdmed.2021.651334

Cited by 8 publications

(1 citation statement)

References 37 publications

(60 reference statements)

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“…While the Ptch1 + cells from FP basal cells get restricted to the apical half of FP, the entire CVP and FOP basal cells continue to express it. Similar to FP, a decline in Ptch1 expression is also observed in the dental inner enamel epithelium to maintain the SHH-responsive quiescent adult stem cells (77). Our previous studies emphasized alterations in apical basal proliferation (25, 26, 28) and elimination of Ptch1 (27) after HH pathway inhibition, implying that Ptch1 controls FP apical cell proliferation.…”

Section: Discussionmentioning

confidence: 94%

Distinct expression patterns of Hedgehog signaling components In mouse gustatory system during postnatal tongue development and adult homeostasis

Kumari,

Franks,

et al. 2023

Preprint

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It is well established that the Hedgehog (HH) pathway regulates embryonic development of anterior tongue taste fungiform papilla (FP) and the posterior circumvallate (CVP) and foliate (FOP) taste papillae, and taste organ maintenance and regeneration in adults. However, there are knowledge gaps in determining HH signaling regulation in postnatal taste organ differentiation and maturation. Importantly, the HH transcription factors GLI1, GLI2 and GLI3 have not been investigated in early postnatal stages; and, the receptors PTCH1, GAS1, CDON and HHIP, required to either drive HH pathway activation or antagonism, remain unexplored. UsinglacZreporter mouse models, we mapped expression of the HH ligand SHH, receptors, and transcription factors in FP, CVP and FOP in early and late postnatal and adult stages. In adults we also studied the soft palate, and the geniculate and trigeminal ganglia which extend afferent fibers to the anterior tongue.ShhandGas1are the only components that were consistently expressed within taste buds of all three papillae and the soft palate. In the first postnatal week, we observed a broad expression of HH signaling components in FP and adjacent, non-taste filiform (FILIF) papillae in epithelium or stroma and tongue muscles. Remarkably, we observed elimination ofGli1in FILIF andGas1in muscles, and downregulation ofPtch1in lingual epithelium and ofCdon,Gas1andHhipin stroma from late postnatal stage. Further, HH receptor expression patterns in CVP and FOP epithelium differed from anterior FP. Among all the components, only known positive regulators of HH signaling, SHH,Ptch1,Gli1andGli2, were expressed in the ganglia. Our studies emphasize differential regulation of HH signaling in distinct postnatal developmental periods and in anterior versus posterior taste organs, and lay the foundation for functional studies to understand the roles of numerous HH signaling components in postnatal tongue development.

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