“…GAPDH was chosen as the internal standard to control for mRNA loading and to normalize TRX. The oligonucleotide sequences used for the forward and reverse primers, melting temperature (Tm in °C), PCR cycle number, and predicted product size (bp) were as follows [14,15]: TRX: forward, 5'-TGAAGCAGATCGAGAGCAAGAC-3', reverse, 5'-TTCATTAATGGRGGCRRCAAGC-3', 60°C, 30 cycles, 305 bp; cTRXR: forward, 5'-GAAGATCTTCCCAAGTCCTATGAC-3', reverse, 5'-ATTTGTTGCCTTAATCCTGTGAGG-3', 64°C, 27 cycles, 420 bp; and GAPDH: forward, 5'-TCACCATCTTCCAGGAGCG-3', reverse, 5'-CTGCTTCACCACCTTCTTGA-3', 60°C, 30 cycles, 572 bp. For each unknown sample, the concentration of TRX, TRXR, and GAPDH was determined from the corresponding standard curves.…”