The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway

An autonomous DNA nanomachine maps spatiotemporal pH changes in a multicellular living organism

“…Th is indicates that at 1 h postinjection, endocytosed I-switch is resident mainly in the lysosomes. Similarly, using GFP RAB-5 as an early endosomal marker 26 and GFP RAB-7 as a late endosomal / lysosomal marker 27 , we determined that the I-switch is resident predominantly in the early and late endosomes at 5 and 17 min, respectively ( Fig. 4a ; Supple mentary Fig.…”

Azithromycin, a Lysosomotropic Antibiotic, Has Distinct Effects on Fluid-Phase and Receptor-Mediated Endocytosis, but Does Not Impair Phagocytosis in J774 Macrophages

“…A second candidate could be Rab5, a rate-limiting catalyst of endocytosis. Dominant-negative Rab5 slows down fluid-phase endocytosis, but it inhibits to a comparable extent the rate of receptor-mediated endocytosis of transferrin [17]. Conversely, dominant-positive Rab5 accelerates phagocytosis [74,75].…”

“…Second, dominant-positive or -negative transfection does not equally affect all cells in a population and may trigger compensatory mechanisms [16]. Third, the effects of transfection can hardly be titrated and may be indirect [17]. Selective inhibitors of endocytosis that cause a rapid and uniform response and allow for titration of its effects therefore remain of great interest.…”

Interplay between receptor tyrosine kinases and hypoxia signaling in cancer

“…Endocytosis consists of a sequence of early and late endosomal fusion events mediated by RAB proteins, a group of RAS-like small guanosine triphosphatases (GTPases) [84], which lead to degradation of internalized receptors in lysosomes. The best studied mediator of early endosome fusion is RAB5 [85]. ccRCC tumors with strong hypoxic signatures were shown to have reduced expression of rabaptin-5, a critical effector of RAB5.…”