The signal metabolite trehalose‐6‐phosphate inhibits the sucrolytic activity of sucrose synthase from developing castor beans

Fedosejevs, Eric T.; Feil, Regina; Lunn, John E.; Plaxton, William C.

doi:10.1002/1873-3468.13197

Cited by 23 publications

(17 citation statements)

References 44 publications

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“…The trehalose phosphate synthases (TPS) convert glucose-6-phosphate and uridine diphosphate (UDP) glucose into trehalose-6phosphate (T6P) and the subsequent dephosphorization of T6P is catalyzed by trehalose-phosphate phosphatases. A recent study reported that threalose-6-phosphate inhibited sucrose synthase and consequently the sucrose cleavage in castor bean [46]. The T6P may be undergoing a higher conversion into trehalose in young melon due to the greater trehalose-phosphate phosphatase gene expression.…”

Section: Global Characteristics Of the 'Yellow' Non-climacteric Melonmentioning

confidence: 99%

Transcriptome profiling of non-climacteric ‘yellow’ melon during ripening: insights on sugar metabolism

et al. 2020

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Background: The non-climacteric 'Yellow' melon (Cucumis melo, inodorus group) is an economically important crop and its quality is mainly determined by the sugar content. Thus, knowledge of sugar metabolism and its related pathways can contribute to the development of new field management and post-harvest practices, making it possible to deliver better quality fruits to consumers.Results: The RNA-seq associated with RT-qPCR analyses of four maturation stages were performed to identify important enzymes and pathways that are involved in the ripening profile of non-climacteric 'Yellow' melon fruit focusing on sugar metabolism. We identified 895 genes 10 days after pollination (DAP)-biased and 909 genes 40 DAP-biased. The KEGG pathway enrichment analysis of these differentially expressed (DE) genes revealed that 'hormone signal transduction', 'carbon metabolism', 'sucrose metabolism', 'protein processing in endoplasmic reticulum' and 'spliceosome' were the most differentially regulated processes occurring during melon development. In the sucrose metabolism, five DE genes are up-regulated and 12 are down-regulated during fruit ripening. Conclusions:The results demonstrated important enzymes in the sugar pathway that are responsible for the sucrose content and maturation profile in non-climacteric 'Yellow' melon. New DE genes were first detected for melon in this study such as invertase inhibitor LIKE 3 (CmINH3), trehalose phosphate phosphatase (CmTPP1) and trehalose phosphate synthases (CmTPS5, CmTPS7, CmTPS9). Furthermore, the results of the protein-protein network interaction demonstrated general characteristics of the transcriptome of young and full-ripe melon and provide new perspectives for the understanding of ripening.

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Section: Global Characteristics Of the 'Yellow' Non-climacteric Melonmentioning

confidence: 99%

Transcriptome profiling of non-climacteric ‘yellow’ melon during ripening: insights on sugar metabolism

et al. 2020

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“…Previously, the link between sucrose and Tre-6P was not fully clear. Nonetheless, it was recently elucidated that Tre-6P regulates sucrose levels by inhibiting the cleavage of sucrose by sucrose synthase (SUS) in castor beans (Ricinus communis) (Fedosejevs et al, 2018). This feedback inhibition may control sucrolytic flux from the source to the sink.…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of Class I Trehalose Biosynthesis Genes in Physcomitrella patens

et al. 2020

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The function of trehalose metabolism in plants during growth and development has been extensively studied, mostly in the eudicot Arabidopsis thaliana. So far, however, not much is known about trehalose metabolism in the moss Physcomitrella patens. Here, we show that in P. patens, two active trehalose-6-phosphate synthase enzymes exist, PpTPS1 and PpTPS2. Expression of both enzymes in Saccharomyces cerevisiae can complement the glucose-growth defect of the yeast tps1Δ mutant. Truncation of N-terminal extension in PpTPS1 and PpTPS2 resulted in higher TPS activity and high trehalose levels, upon expression in yeast. Physcomitrella knockout plants were generated and analyzed in various conditions to functionally characterize these proteins. tps1Δ and tps2Δ knockouts displayed a lower amount of caulonema filaments and were significantly reduced in size of gametophores as compared to the wild type. These phenotypes were more pronounced in the tps1Δ tps2Δ mutant. Caulonema formation is induced by factors such as high energy and auxins. Only high amounts of supplied energy were able to induce caulonema filaments in the tps1Δ tps2Δ mutant. Furthermore, this mutant was less sensitive to auxins as NAA-induced caulonema development was arrested in the tps1Δ tps2Δ mutant. In contrast, formation of caulonema filaments is repressed by cytokinins. This effect was more severe in the tps1Δ and tps1Δ tps2Δ mutants. Our results demonstrate that PpTPS1 and PpTPS2 are essential for sensing and signaling sugars and plant hormones to monitor the balance between caulonema and chloronema development.

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“…which also has high interaction with two alpha-trehalose phosphate synthase isoenzymes (CmTPS7, CmTPS5 [45]. The T6P may be undergoing a higher conversion into trehalose in young melon due to the greater trehalose-phosphate phosphatase gene expression.…”

Section: Global Characteristics Of the 'Yellow' Non-climacteric Melonmentioning

confidence: 99%

Transcriptome profiling of non-climacteric ‘Yellow’ melon during ripening: insights on sugar metabolism

Schemberger

Stroka

Reis

et al. 2020

Preprint

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Background: The non-climacteric ‘Yellow’ melon ( Cucumis melo , inodorus group) is an economically important crop and its quality is mainly determined by the sugar content. Thus, knowledge of sugar metabolism and its related pathways can contribute to the development of new field management and post-harvest practices, making it possible to deliver better quality fruits to consumers. Results: The RNA-seq associated with RT-qPCR analyses of four maturation stages were performed to identify important enzymes and pathways that are involved in the ripening profile of non-climacteric ‘Yellow’ melon fruit focusing on sugar metabolism. We identified 895 genes 10 days after pollination (DAP)-biased and 909 genes 40 DAP-biased. The KEGG pathway enrichment analysis of these differentially expressed (DE) genes revealed that ‘hormone signal transduction’, ‘carbon metabolism’, ‘sucrose metabolism’, ‘protein processing in endoplasmic reticulum’ and ‘spliceosome’ were the most differentially regulated processes occurring during melon development. In the sucrose metabolism, five DE genes are up-regulated and twelve are down-regulated during fruit ripening. Conclusions: The results demonstrated important enzymes in the sugar pathway that are responsible for the sucrose content and maturation profile in non-climacteric ‘Yellow’ melon. New DE genes were first detected for melon in this study such as invertase inhibitor LIKE 3 ( CmINH3 ), trehalose phosphate phosphatase ( CmTPP1 ) and trehalose phosphate synthases ( CmTPS5 , CmTPS7 , CmTPS9 ). Furthermore, the results of the protein-protein network interaction demonstrated general characteristics of the transcriptome of young and full-ripe melon and provide new perspectives for the understanding of ripening.

show abstract

The signal metabolite trehalose‐6‐phosphate inhibits the sucrolytic activity of sucrose synthase from developing castor beans

Cited by 23 publications

References 44 publications

Transcriptome profiling of non-climacteric ‘yellow’ melon during ripening: insights on sugar metabolism

Transcriptome profiling of non-climacteric ‘yellow’ melon during ripening: insights on sugar metabolism

Functional Characterization of Class I Trehalose Biosynthesis Genes in Physcomitrella patens

Transcriptome profiling of non-climacteric ‘Yellow’ melon during ripening: insights on sugar metabolism

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The signal metabolite trehalose‐6‐phosphate inhibits the sucrolytic activity of sucrose synthase from developing castor beans

Cited by 23 publications

References 44 publications

Transcriptome profiling of non-climacteric ‘yellow’ melon during ripening: insights on sugar metabolism

Transcriptome profiling of non-climacteric ‘yellow’ melon during ripening: insights on sugar metabolism

Functional Characterization of Class I Trehalose Biosynthesis Genes in Physcomitrella patens

­Transcriptome profiling of non-climacteric ‘Yellow’ melon during ripening: insights on sugar metabolism

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Transcriptome profiling of non-climacteric ‘Yellow’ melon during ripening: insights on sugar metabolism