The Sequence of a 36·7 kb Segment on the Left Arm of Chromosome IV fromSaccharomyces cerevisiae Reveals 20 Non-overlapping Open Reading Frames (ORFs) IncludingSIT4,FAD1,NAM1,RNA11,SIR2,NAT1,PRP9,ACT2 andMPS1 and 11 New ORFs

Sarén, Ari-Matti; Laamanen, Päivi; Lejarcegui, Javier Beascoechea; Paulín, Lars

doi:10.1002/(sici)1097-0061(199701)13:1<65::aid-yea50>3.0.co;2-t

Cited by 6 publications

References 27 publications

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Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins inSaccharomyces cerevisiae by gene disruption and Northern analysis

Shiratori

Shibata

Arisawa

et al. 1999

Yeast

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Helicase‐related proteins play important roles in various cellular processes incuding DNA replication, DNA repair, RNA processing and so on. It has been well known that the amino acid sequences of these proteins contain several conserved motifs, and that the open reading frames (ORFs) which encode helicase‐related proteins make up several gene families. In this study, we have identified 134 ORFs that encode helicase‐like proteins in the Saccharomyces genome, based on similarity with the ORFs of authentic helicase and helicase‐related proteins. Multiple alignment of the ORF sequences resulted in the 134 ORFs being classified to 11 clusters. Seven out of 21 previously uncharacterized ORFs (YDL031w, YDL070w, YDL084w, YGL150c, YKL078w, YLR276c, and YMR128w) were identified by systematic gene disruption, to be essential for vegetative growth. Three (YDR332w, YGL064c, and YOL095c) out of the remaining 14 dispensable ORFs exhibited the slow‐growth phenotype at 30°C and 37°C. Furthermore, the expression profiles of transcripts from 43 ORFs were examined under seven different growth conditions by Northern analysis and reverse transcription‐polymerase chain reaction, indicating that all of the 43 tested ORFs were transcribed. Interestingly, we found that the level of transcript from 34 helicase‐like genes was markedly increased by heat shock. This suggests that helicase‐like genes may be involved in the biosynthesis of nucleic acids and proteins, and that the genes can be transcriptionally activated by heat shock to compensate for the repressed synthesis of mRNA and protein. Copyright © 1999 John Wiley & Sons, Ltd.

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Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins inSaccharomyces cerevisiae by gene disruption and Northern analysis

Shiratori

Shibata

Arisawa

et al. 1999

Yeast

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Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2)

Doucette

Nazarali

2016

Methods in Molecular Biology

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SIRT2 is a NAD(+)-dependent deacetylase that belongs to the sirtuin family, which is comprised of seven members (SIRT1-SIRT7) in humans. Furthermore, recent study shows that the Sirt2 gene has three transcript variants in mice. Several diverse proteins have been identified as SIRT2 substrates. SIRT2 activity involves multiple cell processes including growth, differentiation, and energy metabolism. However, little is known of SIRT2's role in oligodendrocytes or in the myelin sheath, where it is an important component. Here we describe procedures that detail Sirt2 gene cloning, identification, expression, and biological analysis in cultured cells.

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Protein kinase (dual specificity kinase)

Springer Handbook of Enzymes

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The Sequence of a 36·7 kb Segment on the Left Arm of Chromosome IV fromSaccharomyces cerevisiae Reveals 20 Non-overlapping Open Reading Frames (ORFs) IncludingSIT4,FAD1,NAM1,RNA11,SIR2,NAT1,PRP9,ACT2 andMPS1 and 11 New ORFs

Cited by 6 publications

References 27 publications

Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins inSaccharomyces cerevisiae by gene disruption and Northern analysis

Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins inSaccharomyces cerevisiae by gene disruption and Northern analysis

Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2)

Protein kinase (dual specificity kinase)

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