“…The cytotoxicity of Ag, AgFe and AgCo NPs was assessed in human cells (T47D-breast carcinoma) and rainbow trout cells (RT-gills) using previously published protocols. 46,47 Briefly, both cell lines were exposed for 24 h to various NP concentrations (1,5,10,20,40, and 60 mg L −1 ). The concentrations were selected considering the HC used in the biofilm deterioration experiments as the middle value.…”