The SCOOP12 peptide regulates defense response and root elongation in <i>Arabidopsis thaliana</i>

Gully, Kay; Pelletier, Sandra; Guillou, Marie-Charlotte; Ferrand, Marina; Aligon, Sophie; Pokotylo, Igor; Perrin, Adrien; Vergne, Emilie; Fagard, Mathilde; Ruelland, Éric; Grappin, Philippe; Bucher, Etienne; Renou, Jean-Pierre; Aubourg, Santiago P.

doi:10.1093/jxb/ery454

Cited by 70 publications

(146 citation statements)

References 77 publications

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“…1 ). As a way to test the generality of these observations, other microbial and plant elicitors were tested, including the recently identified SERINE RICH ENDOGENOUS PEPTIDE 12 (SCOOP12) peptide 12 that is proposed to function as a phytocytokine (a secreted peptide that regulates plant immune responses by analogy to cytokines in metazoans 13 ). Surprisingly, unlike what was observed with flg22 or Pep1 (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Perception of a divergent family of phytocytokines by the Arabidopsis receptor kinase MIK2

et al. 2021

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Plant genomes encode hundreds of receptor kinases and peptides, but the number of known plant receptor-ligand pairs is limited. We report that the Arabidopsis leucine-rich repeat receptor kinase LRR-RK MALE DISCOVERER 1-INTERACTING RECEPTOR LIKE KINASE 2 (MIK2) is the receptor for the SERINE RICH ENDOGENOUS PEPTIDE (SCOOP) phytocytokines. MIK2 is necessary and sufficient for immune responses triggered by multiple SCOOP peptides, suggesting that MIK2 is the receptor for this divergent family of peptides. Accordingly, the SCOOP12 peptide directly binds MIK2 and triggers complex formation between MIK2 and the BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) co-receptor. MIK2 is required for resistance to the important root pathogen Fusarium oxysporum. Notably, we reveal that Fusarium proteomes encode SCOOP-like sequences, and corresponding synthetic peptides induce MIK2-dependent immune responses. These results suggest that MIK2 may recognise Fusarium-derived SCOOP-like sequences to induce immunity against Fusarium. The definition of SCOOPs as MIK2 ligands will help to unravel the multiple roles played by MIK2 during plant growth, development and stress responses.

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Section: Resultsmentioning

confidence: 99%

Perception of a divergent family of phytocytokines by the Arabidopsis receptor kinase MIK2

et al. 2021

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“…This launches a positive feedback loop, as F‐actin activates phospholipase D of Nicotiana tabacum , while G‐actin inhibits it in vitro (Pleskot et al , 2010). This fits together with the fact that a PA increase follows the recognition of MAMPs and DAMPs, but also the application of salicylic acid (SA) (Kalachova et al , 2013; Cacas et al , 2017; Gully et al , 2019). At the same time, chemical disruption of actin also triggers SA accumulation and SA‐responsive genes (Matouskova et al , 2014; Kalachova et al , 2019; Leontovycova et al , 2019).…”

Section: Figmentioning

confidence: 76%

Disrupted actin: a novel player in pathogen attack sensing?

2020

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The actin cytoskeleton is widely involved in plant immune responses. The majority of studies show that chemical disruption of the actin cytoskeleton increases plant susceptibility to pathogen infection. Similarly, several pathogens have adopted this as a virulence strategy and produce effectors that affect cytoskeleton integrity. Such effectors either exhibit actin-depolymerizing activity themselves or prevent actin polymerization. Is it thus possible for plants to recognize the actin's status and launch a counterattack? Recently we showed that chemical depolymerization of actin filaments can trigger resistance to further infection via the specific activation of salicylic acid (SA) signalling. This is accompanied by several defence-related, but SA-independent, effects (e.g. callose deposition, gene expression), relying on vesicular trafficking and phospholipid metabolism. These data suggest that the role of actin in plant-pathogen interactions is more complex than previously believed. It raises the question of whether plants have evolved a mechanism of sensing pathological actin disruption that eventually triggers defence responses. If so, what is the molecular basis of it? Otherwise, why does actin depolymerization specifically influence SA content but not any other phytohormone? Here we propose an updated model of actin's role in plant-microbe interactions and suggest some future directions of research to be conducted in this area.

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“…As shown in Figure 4c, MIK2 ECD bound SCOOP12 potently with a dissociation constant (Kd) of 3.18 µM. Alanine (A) substitutions on two conserved serine (S) residues in the SxS motif of SCOOP12 (SCOOP12 SS / AA ) abolished its activities to inhibit root growth through MIK2 ( Supplementary Figure 4d, e) (Gully et al, 2019). No binding was detected between MIK2 ECD and SCOOP12 SS / AA (Figure 4d).…”

Section: Mik2 Is the Receptor Of Scoop12mentioning

confidence: 94%

“…SCOOP15 was also named as STMP8 (Yu et al, 2020). Thus, the Arabidopsis genome encodes at least 23 SCOOP isoforms characterized by a conserved C-terminus with an SxS motif (where S is serine and x is any amino acid) (Figure 2a, Supplementary Figure 2a) 21 . Some SCOOPs, including SCOOP6, 7, 10, 11, and 15, contain two copies (an uppercase A or B was added for each copy) of the conserved domain with an SxS motif (Figure 2a).…”

Section: Multiple Scoop Peptides Activate Diverse Plant Immune and Phmentioning

confidence: 99%

“…It is generally believed that the immune response triggered by DAMPs is to amplify MAMP-activated defense since DAMPs are usually induced upon MAMP perception 9,18,19 . Some plant secreted peptides, such as SERINE-RICH ENDOGENOUS PEPTIDE 12 (SCOOP12), have been shown to induce typical MAMP/DAMP-triggered immune response, the receptors of which have not been identified 21 .…”

Section: Introductionmentioning

confidence: 99%

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Immune elicitation by sensing the conserved signature from phytocytokines and microbes via theArabidopsisMIK2 receptor

Hou

Liu

Huang

et al. 2021

Preprint

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Sessile plants encode a large number of small peptides and cell surface-resident receptor kinases, most of which have unknown functions. Here, we report that the Arabidopsis receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) recognizes the conserved signature motif of SERINE RICH ENDOGENOUS PEPTIDEs (SCOOPs) from plants as well as proteins present in fungal Fusarium spp. and bacterial Comamonadaceae, and elicits potent immune responses. SCOOP signature peptides trigger diverse immune and physiological responses in a MIK2-dependent manner with a sub-nanomolar sensitivity and directly bind to the extracellular leucine rich-repeat domain of MIK2 in vivo and in vitro, indicating that MIK2 is the receptor of SCOOP peptides. Perception of SCOOP peptides induces the association of MIK2 and the coreceptors SOMATIC EMBRYOGENESIS RECEPTOR KINASE 3 (SERK3) and SERK4 and relays the signaling through the cytosolic receptor-like kinases BOTRYTIS-INDUCED KINASE 1 (BIK1) and AVRPPHB SUSCEPTIBLE1 (PBS1)-LIKE 1 (PBL1). Our study identified a unique plant receptor that bears a dual recognition capability sensing the conserved peptide motif from phytocytokines and microbial proteins via a convergent signaling relay to ensure a robust immune response.

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The SCOOP12 peptide regulates defense response and root elongation in Arabidopsis thaliana

Abstract: A secreted peptide, a member of a Brassicaceae-specific gene family, acts on pathogen defense response and root development through the phospholipid pathway and ROS regulation.

Cited by 70 publications

References 77 publications

Perception of a divergent family of phytocytokines by the Arabidopsis receptor kinase MIK2

Perception of a divergent family of phytocytokines by the Arabidopsis receptor kinase MIK2

Disrupted actin: a novel player in pathogen attack sensing?

Immune elicitation by sensing the conserved signature from phytocytokines and microbes via theArabidopsisMIK2 receptor

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