The Salmonella-containing vacuole—Moving with the times

Steele‐Mortimer, Olivia

doi:10.1016/j.mib.2008.01.002

Cited by 269 publications

(295 citation statements)

References 79 publications

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“…For comparison, we also monitored distribution of another LGP, LAMP1 (lysosomal-associated membrane protein 1) in infected HeLa epithelial cells. In agreement with previous studies, 34,36 the infected epithelial cell showed long SIF connected to LAMP1-positive SCV (Fig. 1A).…”

Section: S Typhimurium Induces In Fibroblasts Aggregates Enriched Insupporting

confidence: 93%

“…16,17 We hypothesized that a distinct SCV biogenesis could be responsible for this nonproliferative state. As SCV accumulate lysosomal membrane glycoproteins (LGP) in epithelial cells, 22,34,36 we examined the distribution of SCARB2/Limp-II, a lysosomal integral membrane protein in S. Typhimurium-infected NRK-49F fibroblasts, fixed and processed for immunofluorescence microscopy. For comparison, we also monitored distribution of another LGP, LAMP1 (lysosomal-associated membrane protein 1) in infected HeLa epithelial cells.…”

Section: S Typhimurium Induces In Fibroblasts Aggregates Enriched Inmentioning

confidence: 99%

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Intracellular Salmonella induces aggrephagy of host endomembranes in persistent infections

et al. 2016

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Xenophagy has been studied in epithelial cells infected with Salmonella enterica serovar Typhimurium (S. Typhimurium). Distinct autophagy receptors target this pathogen to degradation after interacting with ubiquitin on the surface of cytosolic bacteria, and the phagophore-and autophagosome-associated protein MAP1LC3/LC3. Glycans exposed in damaged phagosomal membranes and diacylglycerol accumulation in the phagosomal membrane also trigger S. Typhimurium xenophagy. How these responses control intraphagosomal and cytosolic bacteria remains poorly understood. Here, we examined S. Typhimurium interaction with autophagy in fibroblasts, in which the pathogen displays limited growth and does not escape into the cytosol. Live-cell imaging microscopy revealed that S. Typhimurium recruits late endosomal or lysosomal compartments that evolve into a membranous aggregate connected to the phagosome. Active dynamics and integrity of the phagosomal membrane are requisite to induce such aggregates. This membranous structure increases over time to become an aggresome that engages autophagy machinery at late infection times (> 6 h postentry). The newly formed autophagosome harbors LC3 and the autophagy receptor SQSTM1/p62 but is devoid of ubiquitin and the receptor CALCOCO2/NDP52. Live-cell imaging showed that this autophagosome captures and digests within the same vacuole the aggresome and some apposed intraphagosomal bacteria. Other phagosomes move away from the aggresome and avoid destruction. Thus, host endomembrane accumulation resulting from activity of intracellular S. Typhimurium stimulates a novel type of aggrephagy that acts independently of ubiquitin and CALCOCO2, and destroys only a few bacteria. Such selective degradation might allow the pathogen to reduce its progeny and, as a consequence, to establish persistent infections.

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Section: S Typhimurium Induces In Fibroblasts Aggregates Enriched Insupporting

confidence: 93%

Section: S Typhimurium Induces In Fibroblasts Aggregates Enriched Inmentioning

confidence: 99%

Intracellular Salmonella induces aggrephagy of host endomembranes in persistent infections

et al. 2016

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“…Previous studies have shown that intracellular pathogens either mimic or target these proteins to establish their niche in the host cells (20,21,24). Similarly, maturation of SCP also depends on the sequential removal and recruitment of different Rabs and syntaxins (2,3,15,19,28). In this study, we have determined the recruitment of different syntaxins on SCP at various stages of their maturation in macrophages and have shown that SCP specifically recruit higher amounts of Syntaxin6 after 90 min of internalization than at 5 min of internalization.…”

Section: Discussionmentioning

confidence: 81%

Salmonella Acquires Lysosome-associated Membrane Protein 1 (LAMP1) on Phagosomes from Golgi via SipC Protein-mediated Recruitment of Host Syntaxin6

Madan¹,

Rastogi²,

Parashuraman³

et al. 2012

Journal of Biological Chemistry

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Background: Intracellular pathogens avoid lysosomal targeting but recruit lysosome-associated membrane protein 1 (LAMP1), the mechanism of which remains unclear. Results: SipC binds with Syntaxin6 and acquires LAMP1 on the phagosome by fusing with Golgi-derived vesicles. Conclusion: This is a novel mechanism by which Salmonella acquires LAMP1 from Golgi. Significance: This is the first demonstration showing how an intracellular pathogen like Salmonella recruits LAMP1 without fusing with lysosomes.

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“…typhimurium is an intracellular Gram-negative pathogenic agent that survives in a special vacuole called the Salmonellacontaining vacuole (SCV) after internalization into the macrophages or epithelial cells. 109 Two different T3SSs, T3SS1 and T3SS2, encoded by pathogenicity island-1 (SPI-1) and SPI-2, respectively, are employed by Sa. typhimurium to secrete effector proteins.…”

Section: Shigella Flexnerimentioning

confidence: 99%

The ubiquitin system: a critical regulator of innate immunity and pathogen–host interactions

Chai

Liu

2016

Cell Mol Immunol

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The ubiquitin system comprises enzymes that are responsible for ubiquitination and deubiquitination, as well as ubiquitin receptors that are capable of recognizing and deciphering the ubiquitin code, which act in coordination to regulate almost all host cellular processes, including host-pathogen interactions. In response to pathogen infection, the host innate immune system launches an array of distinct antimicrobial activities encompassing inflammatory signaling, phagosomal maturation, autophagy and apoptosis, all of which are fine-tuned by the ubiquitin system to eradicate the invading pathogens and to reduce concomitant host damage. By contrast, pathogens have evolved a cohort of exquisite strategies to evade host innate immunity by usurping the ubiquitin system for their own benefits. Here, we present recent advances regarding the ubiquitin system-mediated modulation of host-pathogen interplay, with a specific focus on host innate immune defenses and bacterial pathogen immune evasion.

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The Salmonella-containing vacuole—Moving with the times

Cited by 269 publications

References 79 publications

Intracellular Salmonella induces aggrephagy of host endomembranes in persistent infections

Intracellular Salmonella induces aggrephagy of host endomembranes in persistent infections

Salmonella Acquires Lysosome-associated Membrane Protein 1 (LAMP1) on Phagosomes from Golgi via SipC Protein-mediated Recruitment of Host Syntaxin6

The ubiquitin system: a critical regulator of innate immunity and pathogen–host interactions

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