The salivary microbiome of diabetic and non‐diabetic adults with periodontal disease

Sabharwal, Amarpreet; Ganley, Kevin; Miecznikowski, Jeffrey C.; Haase, Elaine M.; Barnes, Virginia Monsul; Scannapieco, Frank A.

doi:10.1002/jper.18-0167

Cited by 62 publications

(71 citation statements)

References 46 publications

(105 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All the disease groups displayed higher microbiota diversity (not conclusive for the T2DM group) and different patterns of microbial communities compared to the Health group, except for the Met group. Several previous reports also disclosed consistent results 17 , 22 , 29 . Generally speaking, the diversity and the relative abundance of oral bacteria increase in periodontitis patients with or without type-2 diabetes mellitus.…”

Section: Discussionsupporting

confidence: 82%

“…Due to the small sample size of the T2DM group, we found a recent paper that consists of patients with highly similar inclusion criteria with our T2DM and DAP group and has its sequencing data of saliva samples available to serve as a validation set to validate the reliability of the group data 22 . High quality reads with a sequencing depth of 30,000 was screened and a final validation set consisting of 16 T2DM patients and 15 DAP patients was obtained.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Alteration of salivary microbiome in periodontitis with or without type-2 diabetes mellitus and metformin treatment

Sun

et al. 2020

Sci Rep

View full text Add to dashboard Cite

We aimed to explore the effects of type-2 diabetes mellitus (T2DM) and hypoglycemic therapy on the salivary microbiome in periodontitis patients and identify the potential salivary micro-biomarker for the early warning of T2DM. Saliva samples were collected from healthy individuals (Health), periodontitis patients (P), T2DM patients, periodontitis patients with T2DM (DAP), and DAP patients treated with Metformin (Met). Samples were determined by16S rRNA gene sequencing. 29 phyla, 322 genera, and 333 species of salivary microbiome were annotated. Compared to the Health group, the P and DAP group showed a significantly higher diversity of saliva microbiota, while the T2DM and Met group had no significant difference in microbial abundance but showed a trend of increasing diversity. Other than well-known periodontitis-inducing pathogens, the proportion of Prevotella copri, Alloprevotella rava, and Ralstonia pickettii, etc. were also significantly increased in periodontitis patients with or without T2DM. After effective glycemic control, the abundance of Prevotella copri, Alloprevotella rava, Ralstonia pickettii, etc. decreased in periodontitis patients with companion T2DM. The accuracies of the classification models in differentiating Health-vs.-P, DAP-vs.-P, and T2DM-vs.-P were 100%, 96.3%, and 98.1%, respectively. Hypoglycemic therapy could reconstruct the saliva microbiota and hence improve the localized conditions of diabetes patients with periodontitis.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Alteration of salivary microbiome in periodontitis with or without type-2 diabetes mellitus and metformin treatment

Sun

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…This diversity difference remained in the young animals even after ligation, while the profiles in the adolescent animals began to approach 60% of the OTUs by 1 month postligation, similar to the maintenance of 60% to 70% of the OTUs during disease in the older animals. This type of finding has been reported for human periodontitis with lower diversity in disease samples, albeit findings vary (62)(63)(64)(65). It has also been reported that while members of the oral microbiome may differ across individuals, the functional genomics of the bacterial population are more similar in health, with substantial changes in the gene expression profiles of commensals with disease (9,20,66).…”

Section: Discussionsupporting

confidence: 60%

Microbiome Profiles of Ligature-Induced Periodontitis in Nonhuman Primates across the Life Span

Kirakodu

Chen

Martinez³

et al. 2019

Infect Immun

View full text Add to dashboard Cite

This investigation compared the microbiomes colonizing teeth during the initiation, progression, and resolution of periodontitis in nonhuman primates (Macaca mulatta) at different ages. Subgingival plaque samples were collected at baseline; 0.5, 1, and 3 months following ligature-induced periodontitis; and following naturally occurring disease resolution at 5 months. Samples were analyzed using 16S amplicon sequencing to identify bacterial profiles across age groups: young (<3 years of age), adolescent (3 to 7 years), adult (12 to 15 years), and aged (17 to 23 years). α-Diversity of the microbiomes was greater in the adult/aged samples than in the young/adolescent samples. β-Diversity of the samples demonstrated clear age group differences, albeit individual variation in microbiomes between animals within the age categories was noted. Phylum distributions differed between the young/adolescent animals and the adult/aged animals at each of the time points, showing an enrichment of the phyla Spirochetes, Fusobacteria, and Bacteroidetes associated with periodontitis. Major differences in the top 50 operational taxonomic units (OTUs) were noted in the young and adolescent microbiomes during initiation and progression postligation compared to the adult and aged animals. The proportions of a large number of species in the top 50 OTUs were lower at baseline and in resolved disease microbiomes in the young samples, while profiles in adolescent animals were more consistent with the disease microbiomes. Microbiome profiles for resolution for adults and aged animals appeared more resilient and generally maintained a pattern similar to that of disease. Use of the model can expand our understanding of the crucial interactions of the oral microbiome and host responses in periodontitis.

show abstract

“…Several studies have compared SM in patients with diabetes to that of healthy controls by means of NGS, and in general, data show that diabetes associates with a decrease in bacterial diversity of SM [66][67][68]. In addition, higher salivary levels of P. gingivalis, T. forsythia and F. alocis were reported in patients with gestational diabetes [69], whereas only minor differences were identified in children with T2DM, when compared to obese and healthy controls, respectively [70].…”

Section: Diabetes and Obesitymentioning

confidence: 99%

The salivary microbiota in health and disease

Belstrøm

2020

Journal of Oral Microbiology

142

112

View full text Add to dashboard Cite

The salivary microbiota (SM), comprising bacteria shed from oral surfaces, has been shown to be individualized, temporally stable and influenced by diet and lifestyle. SM reflects local bacterial alterations of the supragingival and subgingival microbiota, and periodontitis and dental-caries associated characteristics of SM have been reported. Also, data suggest an impact of systemic diseases on SM as demonstrated in patients with a wide variety of systemic diseases including diabetes, cancer, HIV and rheumatoid arthritis. The presence of systemic diseases seems to influence salivary levels of specific bacterial species, as well as αand β-diversity of SM. The composition of SM might thereby potentially mirror oral and general health status. The contentious development of advanced molecular techniques such as metagenomics, metatranscriptomics and metabolomics has enabled the possibility to address bacterial functions rather than presence in microbial samples. However, at present only a few studies have employed such techniques on SM to reveal functional and metabolic characteristics in oral health and disease. Future studies are therefore warranted to illuminate the possible impact of metabolic functions of SM on oral and general health status. Ultimately, such an approach has the possibility to reveal novel and personalized therapeutic avenues in oral and general medicine.

show abstract

The salivary microbiome of diabetic and non‐diabetic adults with periodontal disease

Abstract: Although select microbiota increased in both diabetes and periodontal disease progression, these genera decreased in co-existing diabetes and periodontal disease. These findings suggest that the genera abundance continues to change with additional stress imposed by co-existing conditions.

Cited by 62 publications

References 46 publications

Alteration of salivary microbiome in periodontitis with or without type-2 diabetes mellitus and metformin treatment

Alteration of salivary microbiome in periodontitis with or without type-2 diabetes mellitus and metformin treatment

Microbiome Profiles of Ligature-Induced Periodontitis in Nonhuman Primates across the Life Span

The salivary microbiota in health and disease

Contact Info

Product

Resources

About