The Roles of E93 and Kr-h1 in Metamorphosis of Nilaparvata lugens

Li, Kai Long; Yuan, S.-Y.; Nanda, Satyabrata; Wang, Wei Xia; Lai, Feng; Fu, Qiang; Wan, P.-J.

doi:10.3389/fphys.2018.01677

Cited by 36 publications

(51 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At the same time point (i.e., day four of the fifth nymphal stage (N5 D4)), very significantly upregulated SgKr-h1 transcript levels were detected in dsSgE93 -injected locusts in head and fat body samples ( Figure 3 B). Indeed, Kr-h1 is an early JH response gene, which has been reported in several insect species to mediate the metamorphosis-inhibiting status quo action downstream of the JH signal and its receptor Met [ 7 , 9 , 11 , 13 , 31 , 32 ]. Kr-h1 levels normally drop in the final nymphal stage, when JH titers are low, to ensure a final transition to the adult stage [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…The resulting JH-Met-Tai complex induces the expression of JH response genes, including the gene coding for Krüppel-homolog 1 ( Kr-h1 ), a Zn-finger containing transcription factor [ 5 , 6 ]. RNA interference (RNAi) studies have demonstrated the importance of the JH receptor Met, as well as the downstream-acting transcription factor Kr-h1, in mediating the anti-metamorphic effects of JH in the red flour beetle Tribolium castaneum , the German cockroach Blattella germanica , the kissing bug Rhodnius prolixus , the two-spotted cricket Gryllus bimaculatus , the brown planthopper Nilaparvata lugens , the common bed bug Cimex lectularius , the migratory locust Locusta migratoria and the fire bug Pyrrhocoris apterus [ 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

RNAi-Mediated Knockdown of Transcription Factor E93 in Nymphs of the Desert Locust (Schistocerca gregaria) Inhibits Adult Morphogenesis and Results in Supernumerary Juvenile Stages

Gijbels

Marchal

Verdonckt

et al. 2020

IJMS

View full text Add to dashboard Cite

Postembryonic development of insects is coordinated by juvenile hormone (JH) together with ecdysteroids. Whereas the JH early response gene krüppel-homolog 1 (Kr-h1) plays a crucial role in the maintenance of juvenile characteristics during consecutive larval stages, the ecdysteroid-inducible early gene E93 appears to be a key factor promoting metamorphosis and adult morphogenesis. Here, we report on the developmental and molecular consequences of an RNAi-mediated knockdown of SgE93 in the desert locust, Schistocerca gregaria, a hemimetabolan species. Our experimental data show that injection of gregarious locust nymphs with a double-stranded RNA construct targeting the SgE93 transcript inhibited the process of metamorphosis and instead led to supernumerary nymphal stages. These supernumerary nymphal instars still displayed juvenile morphological features, such as a nymphal color scheme and body shape, while they reached the physical body size of the adult locusts, or even surpassed it after the next supernumerary molt. Interestingly, when compared to control locusts, the total duration of the fifth and normally final nymphal (N5) stage was shorter than normal. This appeared to correspond with temporal and quantitative changes in hemolymph ecdysteroid levels, as well as with altered expression of the rate-limiting Halloween gene, Spook (SgSpo). In addition, the levels of the ecdysone receptor (SgEcR) and retinoïd X receptor (SgRXR) transcripts were altered, indicating that silencing SgE93 affects both ecdysteroid synthesis and signaling. Upon knockdown of SgE93, a very potent upregulation of the SgKr-h1 transcript levels was observed in both head and fat body, while no significant changes were detected in the transcript levels of SgJHAMT and SgCYP15A1, the enzymes that catalyze the two final steps in JH biosynthesis. Moreover, the process of molting was disturbed in these supernumerary nymphs. While attempting ecdysis to the next stage, 50% of the N6 and all N7 nymphal instars eventually died. S. gregaria is a very harmful, swarm-forming pest species that destroys crops and threatens food security in many of the world’s poorest countries. We believe that a better knowledge of the mechanisms of postembryonic development may contribute to the discovery of novel, more selective and sustainable strategies for controlling gregarious locust populations. In this context, identification of molecular target candidates that are capable of significantly reducing the fitness of this devastating swarming pest will be of crucial importance.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RNAi-Mediated Knockdown of Transcription Factor E93 in Nymphs of the Desert Locust (Schistocerca gregaria) Inhibits Adult Morphogenesis and Results in Supernumerary Juvenile Stages

Gijbels

Marchal

Verdonckt

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…The Met‐Kr‐h1‐E93 pathway (MEKRE93 pathway) appears to be central to the status quo action of JH in which E9 3 acts downstream of Kr‐h1 to promote adult metamorphosis (Belles and Santos, ; Ureña et al, ). For instance, the knockdown of Kr‐h1 with RNAi in N. lugens resulted in significant up‐regulation of E93 (Li et al, ). Further research is required to identify the molecular mechanism underlying the role of Kr‐h1 in the reproduction of C. suppressalis .…”

Section: Discussionmentioning

confidence: 99%

RNA interference‐mediated knockdown of the transcription factor Krüppel homologue 1 suppresses vitellogenesis in Chilo suppressalis

Tang

et al. 2019

Insect Molecular Biology

View full text Add to dashboard Cite

Vitellogenesis in holometabolous insects involves the production and secretion of vitellogenin (Vg) and other yolk protein precursors in developing oocyte by the fat body, all of which is predominantly orchestrated by juvenile hormone (JH). Krüppel homologue 1 (Kr‐h1) is a zinc finger transcription factor that has been demonstrated to be a JH‐early inducible gene and to contribute to reproduction. However, the exact molecular function of Kr‐h1 in insect reproduction is poorly understood. In the current study, we used the notorious pest Chilo suppressalis as a model system to investigate the role of Kr‐h1 in female reproduction. Cloning and sequencing C. suppressalis Kr‐h1 revealed that it shares high identity with its homologues from other lepidopteran insects. Moreover, RNA interference‐mediated knockdown of CsKr‐h1 substantially reduced the transcription of Vg in the fat body, dramatically decreased yolk protein deposition and also impaired oocyte maturation and ovarian development, indicating that Kr‐h1 is indispensable for normal vitellogenesis in C. suppressalis. Based on these results, we conclude that Kr‐h1 is crucial to reproduction in insects and that targeting this gene could potentially be a new way to suppress rice pests.

show abstract

“…In other holometabolous insects including beetle Tribolium castaneum , moths Bombyx mori and Helicoverpa armigera , as well as hemimetabolous insects including cockroach Blattella germanica , planthopper Nilaparvata lugens , and stinkbug Pyrrhocoris apterus and Rhodnius prolixus , Kr-h1 has also exhibited anti-metamorphic effects ( Minakuchi et al, 2009 ; Konopova et al, 2011 ; Lozano and Belles, 2011 ; Kayukawa et al, 2017 ; Li et al, 2018 ; Zhang W. N. et al, 2018 ).…”

Section: Diverse Roles Of Sesquiterpenoids In Insectsmentioning

confidence: 99%

Diversity of Insect Sesquiterpenoid Regulation

Tsang

Law

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

Insects are arguably the most successful group of animals in the world in terms of both species numbers and diverse habitats. The sesquiterpenoids juvenile hormone, methyl farnesoate, and farnesoic acid are well known to regulate metamorphosis, reproduction, sexual dimorphism, eusociality, and defense in insects. Nevertheless, different insects have evolved with different sesquiterpenoid biosynthetic pathway as well as products. On the other hand, non-coding RNAs such as microRNAs have been implicated in regulation of many important biological processes, and have recently been explored in the regulation of sesquiterpenoid production. In this review, we summarize the latest findings on the diversity of sesquiterpenoids reported in different groups of insects, as well as the recent advancements in the understanding of regulation of sesquiterpenoid production by microRNAs.

show abstract

The Roles of E93 and Kr-h1 in Metamorphosis of Nilaparvata lugens

Cited by 36 publications

References 51 publications

RNAi-Mediated Knockdown of Transcription Factor E93 in Nymphs of the Desert Locust (Schistocerca gregaria) Inhibits Adult Morphogenesis and Results in Supernumerary Juvenile Stages

RNAi-Mediated Knockdown of Transcription Factor E93 in Nymphs of the Desert Locust (Schistocerca gregaria) Inhibits Adult Morphogenesis and Results in Supernumerary Juvenile Stages

RNA interference‐mediated knockdown of the transcription factor Krüppel homologue 1 suppresses vitellogenesis in Chilo suppressalis

Diversity of Insect Sesquiterpenoid Regulation

Contact Info

Product

Resources

About