The role of transcription in EGF- and FSH-mediated oocyte maturation in vitro

Farin, Charlotte E.; Rodriguez, Karina F.; Alexander, Jacob; Hockney, Jessica Eileen; Herrick, Jason R.; Kennedy‐Stoskopf, Suzanne

doi:10.1016/j.anireprosci.2006.10.007

Cited by 47 publications

(44 citation statements)

References 99 publications

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“…Este fator de crescimento é um polipeptídeo encontrado por todo o corpo, inclusive em folículos antrais em crescimento no ovário e estão envolvidos na síntese de DNA e proliferação das células da granulosa (KIM et al, 2004). Além disso, desempenha papel essencial na regulação da maturação e expansão das células do cumulus (FARIN et al, 2007). Bolamba et al (2006), avaliando meio de maturação suplementado com FSH, LH, estradiol e EGF, observaram que este fator de crescimento utilizado isoladamente não é suficiente para promover a maturação dos oócitos e nem promover a expansão das células do cumulus.…”

Section: Discussionunclassified

“…Em felinos, foi observado que a adição no meio de maturação com EGF proporcionou o desenvolvimento do oócito até a M-II e que este fator de crescimento é requerido pelo FSH para a progressão meiótica do oócito (FARIN et al, 2007). Nesta pesquisa, pode-se constatar maiores taxas de M-II nos oócitos maturados em meio suplementado com EGF, somente nos oócitos obtidos da fase de diestro (p < 0,01).…”

Section: Discussionunclassified

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Efeito do fator de crescimento epidermal (EGF) na maturação in vitro de oócitos caninos

Pereira

Bersano

Lopes

2014

Braz. J. Vet. Res. Anim. Sci.

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ResumoEste trabalho teve o objetivo de avaliar a influência do EGF na maturação in vitro (MIV) de cadelas. Realizou-se o transporte dos ovários em solução de cloreto de sódio 0,9%, que foram seccionados em solução salina fosfato tamponado (PBS) e os complexos cumulus oócito (COCs) selecionados em meio de cultura de tecidos (TCM) 199 suplementado com HEPES. Foram coletados 405 oócitos grau 1 de cadelas em anestro e diestro. Os oócitos provenientes das duas fases do ciclo estral foram submetidos a dois tratamentos: meio com adição de 10 ηg/mL do fator de crescimento epidermal (EGF) (T) e meio sem suplementação (C). Depois de 72 horas de maturação, os COCs foram desnudados, fixados e corados com HOESCHT 33342 para avaliação da maturação nuclear. Os oócitos obtidos dos ovários da fase de diestro do grupo T demonstraram maior porcentagem (18,8%) de oócitos no estágio de metáfase II (M-II), em relação ao grupo C (1,3%) (p < 0,01). Nos oócitos obtidos da fase de anestro houve diferença (p < 0,05) entre os grupos, observando-se menor parcela (4,1%) de oócitos no estágio de vesícula germinativa (VG) no grupo T, quando comparado ao grupo C (16,1%). Comparando-se as diferentes fases reprodutivas, em meios suplementados com EGF, foi observada diferença (p < 0,05) apenas nos oócitos obtidos da fase de diestro em relação ao estágio de M-II. Dessa maneira, a suplementação no meio de maturação na concentração de 10 ηg/mL, neste estudo, exerceu influência positiva apenas na MIV de oóci-tos caninos oriundos da fase de diestro, incrementando os índices de M-II nessa espécie. Palavras-chave:Maturação in vitro. EGF. Anestro. Diestro. Cadela. AbstractThis work aimed to evaluate the influence of EGF on canine oocyte in vitro maturation (IVM). We carried out the transport of the ovaries in sodium chloride 0.9% solution, which were cut into phosphate-buffered saline (PBS) and the cumulus oocyte complexes (COCs) selected in tissue culture medium (TCM), supplemented with 199 HEPES. A total of 405 grade 1 oocytes were collected from bitches in anestrus and diestrus. Oocytes from the two phases of estrous cycle were subjected to two treatments: medium with addition of 10 ηg/mL epidermal growth factor (EGF) (T) and medium without supplementation (C). After 72 h of maturation, COCs were denuded, fixed and stained with Hoechst 33342 to assess nuclear maturation. The oocytes obtained from the ovaries of the diestrus phase of the T group demonstrated higher percentage (18.8%) of oocytes at metaphase II stage (M-II) than C group (1.3%) (p < 0.01). The oocytes from anestrus phase demonstrated difference (p < 0.05) between the groups, observing smaller proportion (4.1%) of oocytes at the stage of germinal vesicle (GV) in group T compared to group C (16.1%). Comparing the different reproductive status in media supplemented with EGF difference (p < 0.05) was observed only in oocytes diestrus phase relative to the stage of M-II. Thus, supplementation on maturation medium at a concentration of 10 ηg/mL of EGF in this study had positive influence only o...

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Section: Discussionunclassified

Efeito do fator de crescimento epidermal (EGF) na maturação in vitro de oócitos caninos

Pereira

Bersano

Lopes

2014

Braz. J. Vet. Res. Anim. Sci.

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“…Regarding oocyte maturation, differences exist in the developmental competence of those matured in vivo compared to the in vitro ones (van de Leemput et al, 1999). Furthermore oocytes that have been exposed to a gonadotropin stimulus either in vivo or during in vitro culture are more competent to support embryonic development following fertilization Farin et al, 2007). The presence of receptors for growth factors such as insulin-like growth factor-1(IGF-1) and epidermal growth factor (EGF) in granulosa cells and oocytes suggest their involvement in the maturation process and developmental competence (Feng et al, 1987;Wang et al, 2009;Procházka et al, 2011) at least in early stages (Kelly et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of two methods of in vitro production of ovine embryos using fresh or cryopreserved semen

Romão

Marques²,

Baptista³

et al. 2013

Small Ruminant Research

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a b s t r a c tSuccessful production of high quality blastocysts depends on the use of a culture system that ensures the acquisition of developmental competence by the maturing oocyte followed by an efficient in vitro fertilization. In the present work the effect of FSH and pyruvate in an EGF containing medium for ovine oocyte maturation prior to insemination with fresh (F) or frozen-thawed (FT) semen on embryo developmental competence and cryosurvival was determined. Sheep oocytes were matured in two culture media (M1 and M2, respectively; M1 = CM + EGF, n = 836 and M2 = CM + EGF + pyruvate + FSH, n = 850) for 22 h and then fertilized using FT or F spermatozoa (M1 × FT = 371, M2 × FT = 359, M1 × F = 353 and M2 × F = 372, 9 replicates) from Merino rams (n = 3). After embryo culture and evaluation, good quality blastocysts (grade 1) were vitrified in OPS. Post-thawed embryo integrity, re-expansion and number of total and viable cells were assessed. Oocyte maturation rates presented no differences (P > 0.05) between treatments (M1 = 87.0 ± 4.1 and M2 = 86.7 ± 3.9%) as well as embryo developmental rates either for maturation media or semen status. However, fresh semen improved blastocyst quality (grade 1 embryos F = 52.5 ± 4.8% and FT = 39.0 ± 4.4%, P = 0.01). Grade 1 blastocysts presented similar post-thawed integrity and re-expansion rates. After 3 h of culture, expansion rates were higher (P = 0.05) for M2 × F warmed embryos (80.0 ± 8.3%) than for M1 × F (54.3 ± 10.4%). Results seem to confirm the existence of a synergistic effect between FSH, EGF and pyruvate upon cytoplasmic maturation of ovine oocytes. Moreover, in vitro fertilization by fresh semen clearly improves ovine embryo developmental competence by enhancing morphological blastocyst quality. The beneficial effect of M2 on cryosurvival was only observed in embryos derived from fresh semen. Therefore these combined strategies enhance embryo cryosurvival.

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“…Because inhibiting PTGS2 during IVM can decrease cumulus expansion, oocyte maturation, and ultimately embryo production (Nuttinck et al 2011), we predicted that a seasonal difference in the production of resulting enzyme may indicate altered FSH function. EGF can promote oocyte maturation (Farin et al 2007, Hatoya et al 2009, Uhm et al 2010 and is necessary for FSH-induced meiotic resumption (Chen et al 2008). This is most likely achieved through FSHinduced upregulation of EGF receptor (EGFR) mRNA expression in cumulus cells (bovine, Assidi et al 2008;goat, Almeida et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Absence of seasonal changes in FSHR gene expression in the cat cumulus–oocyte complex in vivo and in vitro

Hobbs

Howard²,

Wildt³

et al. 2012

Reproduction

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Domestic cat oocytes are seasonally sensitive to FSH. Compared with those collected during the breeding season, oocytes from the nonbreeding (NB) season require more FSH during in vitro maturation to achieve comparable developmental competence. This study tested the hypothesis that this seasonal variation was due to altered expression of FSH receptors (FSHR) and/or FSH-induced genes. Relative expression levels of FSHR mRNA and FSH-enhanced gene estrogen receptor b (ESR2) were measured by qPCR in whole ovaries and immature cumulus-oocyte complexes (COCs) isolated from cat ovaries during the natural breeding vs NB seasons. Expression levels of FSH-induced genes prostaglandin-endoperoxide synthase 2 (PTGS2), early growth response protein-1 (EGR1), and epidermal growth factor receptor (EGFR) were examined in mature COCs from both seasons that were a) recovered in vivo or b) matured in vitro with conventional (1 mg/ml) or high (10 mg/ml) FSH concentrations. Overall, FSHR mRNA levels were lower in whole ovaries during the NB compared with breeding season but were similar in immature COCs, whereas ESR2 levels did not differ in either group between intervals. We observed changes in PTGS2, EGR1, and EGFR mRNA expression patterns across maturation in COCs within but not between the two seasons. The lack of seasonal differentiation in FSH-related genes was not consistent with the decreased developmental capacity of oocytes fertilized during the NB season. These findings reveal that the seasonal decrease in cat oocyte sensitivity to FSH occurs both in vivo and in vitro. Furthermore, this decline is unrelated to changes in expression of FSHR mRNA or mRNA of FSH-induced genes in COCs from antral follicles.

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The role of transcription in EGF- and FSH-mediated oocyte maturation in vitro

Cited by 47 publications

References 99 publications

Efeito do fator de crescimento epidermal (EGF) na maturação in vitro de oócitos caninos

Efeito do fator de crescimento epidermal (EGF) na maturação in vitro de oócitos caninos

Evaluation of two methods of in vitro production of ovine embryos using fresh or cryopreserved semen

Absence of seasonal changes in FSHR gene expression in the cat cumulus–oocyte complex in vivo and in vitro

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