The Role of Specific Suppressor T Cells in Immune Tolerance

Nachtigal, D.; Zan‐Bar, Israel; Feldman, Michaël

doi:10.1111/j.1600-065x.1975.tb00176.x

Cited by 36 publications

(30 citation statements)

References 31 publications

(9 reference statements)

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“…The converse situation in which suppressor cells exist without tolerance is documented by the presence of suppressor cells in HGG-primed mice (18). In parallel to the data presented here, recent observations (37) suggest that suppressor cells are coincidental to, but not obligate for, tolerance to another soluble protein antigen human serum albumin in which suppressor T cells have been well documented (38).…”

Section: Discussionsupporting

confidence: 73%

Induction and mode of action of suppressor cells generated against human gamma globulin. I. An immunologic unresponsive state devoid of demonstrable suppressor cells.

Parks¹,

Doyle²,

Weigle³

1978

The Journal of Experimental Medicine

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A model of unresponsiveness to human gamma-globulin (HGG) which is maintained in the absence of demonstrable suppressor cells has been described. A/J mice were tolerized with deaggregated HGG purified from a variety of sources. The spleen cells from these tolerized mice were assessed for their ability to suppress the response of normal spleen cells to HGG when transferred into lethally irradiated mice. All of the HGG preparations obtained from commercial sources as Cohn fraction II of pooled, outdated plasma induced suppressor cells to HGG, although not of equal magnitude. However, suppressor cells could not be demonstrated in the spleens of mice tolerized with deaggregated HGG purified from the plasma of a healthy individual. This inability to detect suppression was independent of the method of purification of the HGG and of the time of assessment of the putative suppressor cells after tolerization. Similarly, deaggregated HGG isolated from an IgG1 lambda-myeloma protein induced unresponsiveness to HGG but did not stimulate demonstrable suppressor cells. These data suggest that suppressor T cells are not involved in the maintenance of tolerance to this antigen, although they may play a regulatory role in the immune response to HGG. Support for this concept was obtained by assessing the duration of unresponsiveness in the T and B lymphocytes of mice tolerized with the various HGG preparations. Mice tolerized with the HGG preparations that stimulated little or no suppression were among the last to recover responsiveness. Indeed, there was no consistent correlation between the level of suppressor cell activity and the degree of unresponsiveness in either the splenic T or B lymphocytes. Thus, although certain HGG preparations may provide a tool for the generation of antigen-specific suppressor T cells, the utilization of these suppressive preparations may be inappropriate for the investigation of the mechanisms of the induction and maintenance of the unresponsive state.

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Section: Discussionsupporting

confidence: 73%

Induction and mode of action of suppressor cells generated against human gamma globulin. I. An immunologic unresponsive state devoid of demonstrable suppressor cells.

Parks¹,

Doyle²,

Weigle³

1978

The Journal of Experimental Medicine

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“…Unresponsiveness lacking suppressor cells has also been reported in adult animals (12)(13)(14) with the conventional DAGG tolerization protocol. The demonstration that tolerance can be induced in congenitally athymic nude mice devoid of competent T cells with both immunogenic (21) and tolerogenic (19,20) forms of heterologous gamma globulins and in adult thymectomized, X-irradiated, bone marrow-reconstituted mice (4,13) further support the postulate that tolerance can be induced in the absence of suppressor T cells. These observations coupled with the demonstration of the presence of antigen-specific-suppressor cells to HGG in primed mice (23) and the more efficient suppression of HGG-primed cells compared to normal cells (76) suggest that these suppressor cells may represent a normal regulatory mechanism operative during antigenic stimulation although their relevance to the unresponsive state to HGG must remain dubious.…”

Section: Inability Of Col Tosupporting

confidence: 49%

“…Although the role of suppressor cells in the establishment of tolerance has been convincingly demonstrated in some unresponsive states (8)(9)(10)(11), this relationship remains to be proven in many systems. Several workers have reported that unresponsiveness to protein antigens can be established in the absence of detectable suppressor ceils in normal animals (12)(13)(14)(15)(16)(17), neonatal mice (18), adult thymectomized, X-irradiated, bone-marrow reconstituted mice (4,13), and athymic nude mice (19)(20)(21). Although antigen-specific-suppressor cells may not be necessary for the establishment or maintenance of these tolerant states, their presence in antigen-primed animals (22,23) suggests that they may play a regulatory role in immune responsiveness to these protein antigens.…”

mentioning

confidence: 99%

Induction and mode of action of suppressor cells generated against human gamma globulin. II. Effects of colchicine.

Parks¹,

Shaller²,

Weigle³

1979

The Journal of Experimental Medicine

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The ability of colchicine (Col) to interfere with suppressor cells specific for the soluble protein antigen human gamma globulin (HGG) has been examined. This interference may be the mechanism of the adjuvanticity promoted by Col. When injected into A/J mice at the appropriate time and concentration, both Col and cyclophosphamide promoted an adjuvant increase in the plaque-forming cell response to 100 micrograms of immunogenic, aggregated HGG. Col abrogated both the induction of suppressor cells when injected with 3 h of tolerization with deaggregated (DHGG) and the expression of previously induced suppressor cells when injected with the antigenic challenge. Interference with the generation and expression of antigen-specific suppressor cells had no detectable effects on the immunologic unresponsive state to HGG. Col did not interfere with the induction of tolerance at a dose (1 mg/kg) that abolished the generation of suppressor cells. Furthermore, the absence of colchicine-sensitive-suppressor cells during the establishment of tolerance had no observable effect on the duration of unresponsivness in either helper T- or B-lymphocyte populations. Finally, Col was not able to terminate the unresponsive state established by DHGG even when responsive splenic B cells could be demonstrated in tolerant animals. These data indicate that suppressor cells are not required for the establishment and maintenance of the unresponsive state to this antigen.

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“…Taken together, these experiments sug gested that the pronounced Id+ response to Rb04/IFA was completely devoid of any binding specificity with the MLSE-derived 35kd antigen. The following experiment was set up to investigate whether the Jd+ response to Rb04/IFA could be regulated by mechanisms which operate in murine responses to protein antigens; na mely, that the response to antigen-adj uvant injection can be abrogated by prior injection of soluble antigen (33,34). Soluble Rb04 (2x50p,1) was given prior to challenge of mice with Rb04/IFA ( …”

Section: Expression Of Ids In Genetically Defined Mouse Strainsmentioning

confidence: 99%

Analysis of idiotypes expressed by anti-mycobacterial mouse monoclonal antibodies using rabbit antisera

Iványi¹,

Praputpittaya²

1986

Leprosy Review

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Rabbit antisera against twelve mouse monoclonal antibodies to mycobacterial antigens showed idiotype-specificity following cross-absorption with normal mouse globulin. The functional aspects have been partly evaluated so far with two of these reagents. The represen tation of ML04 idiotype appeared to be associated with Igh alleles. Injection of mice with Rb04 anti-Id in incomplete Freunds adjuvant induced Id+ serum levels but without corres ponding antigen binding specificity. In contrast, Rb7 1 anti-Id revealed internal image pro perties corresponding to the homologous 38kd protein antigen of tubercle bacilli. This was demonstrated by the potency of Rb7 1 to sensitize mice in vivo and to elicit T cell prolifera tive and DTH responses.

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The Role of Specific Suppressor T Cells in Immune Tolerance

Cited by 36 publications

References 31 publications

Induction and mode of action of suppressor cells generated against human gamma globulin. I. An immunologic unresponsive state devoid of demonstrable suppressor cells.

Induction and mode of action of suppressor cells generated against human gamma globulin. I. An immunologic unresponsive state devoid of demonstrable suppressor cells.

Induction and mode of action of suppressor cells generated against human gamma globulin. II. Effects of colchicine.

Analysis of idiotypes expressed by anti-mycobacterial mouse monoclonal antibodies using rabbit antisera

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