The role of resveratrol in bone marrow‐derived mesenchymal stem cells from patients with osteoporosis

Abstract

The aim of the present study was to investigate the effects of resveratrol on BMSCs from patients with osteoporosis. The cell viability and proliferation of BMSCs after treatment with different concentrations of resveratrol was respectively observed by MTT assay and EdU staining. The apoptosis was assessed using by TUNEL staining and the pluripotency was analyzed by quantitative reverse transcription‐PCR (qRT‐PCR). The osteogenic differentiation and adipogenic differentiation were determined by alkaline phosphatase (ALP) staining, alizarin red S (ARS) staining, oil red O (ORO) staining and qRT‐PCR analysis. MTT assay showed that Res at 40, 80, 100 μM markedly improved the cell proliferation of BMSCs from patients with osteoporosis. EdU staining indicated that Res treatment significantly accelerated the proliferation of BMSCs. In addition, the results of TUNEL staining revealed that Res at 40, 80, 100 μM inhibited the osteoporosis‐related apoptosis of BMSCs. qRT‐PCR analysis explored that Res treatment played a positive role in the pluripotency in BMSCs. ALP, ARS staining and qRT‐PCR demonstrated that Res promoted the differentiation of BMSCs into osteoblasts, especially at 80 μM. ORO staining and qRT‐PCR analysis proved that treatment of Res inhibited the adipogenesis of BMSCs isolated from patients with osteoporosis. Our findings suggested that Res can play a vital role in the cell viability, proliferation, apoptosis, pluripotency, osteogenesis and adipogenesis of BMSCs. And Res might be an efficient therapeutic approach for treating patients with osteoporosis.

Resveratrol can enhance osteogenic differentiation and mitochondrial biogenesis from human periosteum-derived mesenchymal stem cells

“…These results indicate that resveratrol can enhance osteogenic differentiation of PO-MSCs. In other studies, resveratrol was reported to promote osteogenic differentiation of bone marrow or adipose tissue derived MSCs at higher concentrations (28)(29)(30)(31)(32). However, in this study, PO-MSC viability decreased when resveratrol dose was above 10 μM.…”

“…Among these diverse effects of resveratrol, one notable benefit of resveratrol is to increase mitochondrial biogenesis in mammalian cells (26,27). Moreover, resveratrol also promotes osteogenic differentiation of bone marrow or adipose tissue derived mesenchymal stem cells (28,29).…”

Resveratrol and Celastrol Loaded Collagen Dental Implants Regulate Periodontal Ligament Fibroblast Growth and Osteoclastogenesis of Bone Marrow Macrophages

“…Similarly, Ornstrupetal reported that Res alone stimulated proliferation and differentiation of bone marrow‐derived mesenchymal stem cells. A recent study claimed that Res plays the main role in proliferation, osteogenesis and adipogenesis of bone mesenchymal stem cells and further concluded that Res could recover the bone homeostasis from osteoporosis . The actual molecular signaling mechanism of Res on osteogenic differentiation was recently elucidated by Huang et al .…”

Anti-Aging Effects of Calorie Restriction (CR) and CR Mimetics Based on the Senoinflammation Concept

“…Previous studies have indicated the beneficial effects of sirtuins as the best small molecule that activate sirtuins, which extended lifespan in a yeast model [104][105][106][107][108][109]. Although only the longevity extension effect of resveratrol has been reported in C. elegans and D. melanogaster, many subsequent studies reported that resveratrol intake promotes health and plays a preventive role in age-related diseases, such as cancer [110][111][112][113], atherosclerosis [114,115], arthritis [116,117], cataract [118][119][120], cardiovascular disease [121], hypertension [122,123], type 2 diabetes [124][125][126][127], osteoporosis [128][129][130], and Alzheimer disease [131][132][133]. In clinical studies, resveratrol intake improved the memory capacity of elderly individuals and reduced blood lipid levels in obese and type 2 diabetic patients [134,135].…”