The role of reactive oxygen species in the antibacterial photodynamic treatment: photoinactivation<i>vs</i>proliferation

Topaloğlu, Nermin; Güney, Melike; Aysan, Nuray; Gülsoy, Murat; Yüksel, Şahru

doi:10.1111/lam.12538

Cited by 31 publications

(16 citation statements)

References 30 publications

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“…Our results obtained with VIS+wIRA on planktonic bacteria showed the antimicrobial effect was dependent on the ICG concentration used. Topaloglu et al [68,69] reported on the risk that lower ICG concentrations used during aPDT cannot only eliminate bacteria but actually increase the proliferation rate of bacteria, as demonstrated in in vitro experiments with P. aeruginosa [29]. This was explained by the amount of ROS, which was generated and determined the actual level of bactericidal effect or proliferation.…”

Section: Discussionmentioning

confidence: 99%

Inactivation of Oral Biofilms Using Visible Light and Water-Filtered Infrared a Radiation and Indocyanine Green

et al. 2019

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Aim: To investigate the antimicrobial photodynamic therapy (aPDT) of visible light and water-filtered infrared A radiation in combination with indocyanine green (ICG) on planktonic oral microorganisms as well as on oral biofilm. Methods: The irradiation was conducted for 5 min in combination with ICG. Treatment with chlorhexidine served as a positive control. The number of colony forming units and bacterial vitality were quantified. Results: All tested bacterial strains and salivary bacteria were killed at a level of 3log10. The colony forming units of the initial mature oral biofilms were strongly reduced. The high bactericidal effect of aPDT was confirmed by live/dead staining. Conclusion: The aPDT using visible light and water-filtered infrared A radiation and ICG has the potential to treat periodontitis and peri-implantitis.

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Section: Discussionmentioning

confidence: 99%

Inactivation of Oral Biofilms Using Visible Light and Water-Filtered Infrared a Radiation and Indocyanine Green

et al. 2019

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“…The main working mechanism is the absorption of light energy by the photoreceptors located in the cell membrane or the chromophores in the mitochondria, particularly cytochrome c oxidase (CCO) which is a component of the mitochondrial respiratory chain 13 . Thus, it may induce biostimulation with many consecutive biochemical reactions that result in cell proliferation, migration, adhesion, and apoptosis 14,15 . There are several signaling cascades in eukaryotic cells and low‐level light therapy has to potential to induce these cascades resulting in proliferation and migration.…”

Section: Introductionmentioning

confidence: 99%

“…13 Thus, it may induce biostimulation with many consecutive biochemical reactions that result in cell proliferation, migration, adhesion, and apoptosis. 14,15 There are several signaling cascades in eukaryotic cells and low-level light therapy has to potential to induce these cascades resulting in proliferation and migration. According to the previous reports, it was declared that low power light may stimulate the phosphorylation of tyrosine-protein kinase receptors (TPKR).…”

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confidence: 99%

Comparative analysis of the light parameters of red and near‐infrared diode lasers to induce photobiomodulation on fibroblasts and keratinocytes: An in vitro study

Topaloğlu

Özdemir

Çevik

2020

Photoderm Photoimm Photomed

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Background Photobiomodulation (PBM) depends on the use of non‐ionizing light energy to trigger photochemical changes, particularly in light‐sensitive mitochondrial structures. It triggers proliferation and the metabolic activity of the cells, primarily by utilizing the energy from the near‐infrared to the red wavelength of the light. Purpose This in vitro study has analyzed comparatively the most appropriate energy doses and wavelengths to induce PBM on keratinocytes and fibroblasts for the accelerated wound healing process. Methods 1, 3, and 5 J/cm2 energy densities of 655 and 808‐nm diode lasers were used to promote cell proliferation and wound healing process. Scratch assay and MTT analysis were performed on keratinocytes and fibroblasts for wound closure and cell proliferation after the triple light applications, respectively. Results 655‐nm of wavelength was more successful on keratinocytes to induce wound healing and cell proliferation, whereas 808‐nm of wavelength was so effective on fibroblasts to heal the wounds totally and it induced cell proliferation almost 3 times compared to the untreated control group. Conclusion This study revealed that PBM with 655 and 808 nm of wavelengths was effective to speed up the wound healing process at specific energy densities. In general 808‐nm of wavelength was more successful. However, the proper wavelength and the energy density may differ according to the cell type. Thus, every light parameter should be chosen properly to obtain better outcomes during PBM applications.

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“…The European Research Journal 2021 μM Ce6 caused nearly 50% reduction in cell viability which can be considered as a high dark toxicity and this cannot support idea of aPDT with less side effect in neighboring areas of biological tissues [23].…”

Section: Discussionmentioning

confidence: 94%

Comparison of the effect of ethanol and potassium iodide in antibacterial photodynamic therapy on gram negative pathogens

Avşar

Cagan

Bakay

et al. 2021

The European Research Journal

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Objectives: Antibiotics is the most common treatment for bacterial infections. However, bacteria can change their genetic material, develop antibiotic resistance and cannot be treated. This brings the need for new treatment methods. Antibacterial Photodynamic Therapy is becoming a promising approach to treat bacterial infections. It is based on the use of photosensitizer to be activated by light with an appropriate wavelength and it will result in reactive oxygen species which are responsible for the destruction of pathogens. In this study, Antibacterial Photodynamic Therapy was examined for the treatment of Pseudomonas aeruginosa with the help of ethanol and potassium iodide. Methods: The effect of Chlorin e6-based photoinactivation was studied on Multidrug resistant P. aeruginosa upon irradiation with 655-nm diode laser. Then ethanol and potassium iodide was added to the mechanism separately to increase the efficacy of photoinactivation. After each application, serial dilution method was used for the determination of viable cells. Results: Outcomes showed that only Antibacterial Photodynamic Therapy causes a mortality rate of 75%. Addition of ethanol causes a mortality rate of 93% and addition of potassium iodide causes a mortality rate of 99.9% with less amount of Chlorin e6 and light dose. Conclusions: Chlorin-e6 based photoinactivation did not provide high mortality rate on P. aeruginosa. The use of ethanol and potassium iodide increased the effect of photoinactivation. The highest mortality rate was obtained with potassium iodide. It was understood that potassium iodide was a better concomitant agent to increase the bactericidal effect of Antibacterial Photodynamic Therapy on P. aeruginosa.

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The role of reactive oxygen species in the antibacterial photodynamic treatment: photoinactivationvsproliferation

Cited by 31 publications

References 30 publications

Inactivation of Oral Biofilms Using Visible Light and Water-Filtered Infrared a Radiation and Indocyanine Green

Inactivation of Oral Biofilms Using Visible Light and Water-Filtered Infrared a Radiation and Indocyanine Green

Comparative analysis of the light parameters of red and near‐infrared diode lasers to induce photobiomodulation on fibroblasts and keratinocytes: An in vitro study

Comparison of the effect of ethanol and potassium iodide in antibacterial photodynamic therapy on gram negative pathogens

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