The Role of Promyelocytic Leukemia Zinc Finger (PLZF) and Glial-Derived Neurotrophic Factor Family Receptor Alpha 1 (GFRα1) in the Cryopreservation of Spermatogonia Stem Cells

Shamhari, Asma’ ‘Afifah; Jefferi, Nur Erysha Sabrina; Hamid, Zariyantey Abd; Budin, Siti Balkis; Idris, Mushrifah; Taib, Izatus Shima

doi:10.3390/ijms24031945

Cited by 5 publications

(2 citation statements)

References 220 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine the role of riluzole in the differentiation of spermatogonia, we examined the expressions of GFRα1, PCNA, SYCP3, and TNP-1, key markers involved in spermatogonia proliferation and differentiation. Our findings revealed that a significant decrease in the expression levels of GFRα1 (which is associated with the self-renewal of SSCs (Shamhari et al, 2023)), PCNA (a marker for proliferating cell nuclear antigen (Fu et al, 2019)), SYCP3 (involved in meiosis (Miyamoto et al, 2003)), and TNP-1 (specific to spermatids (Della-Maria, Gerard, Franck, & Gerard, 2002)) after BSF treatment. However, treatment with riluzole, whether administered via intraperitoneal injection or intragastric gavage, significantly increased the expression of these marks (Figure 3A-D).…”

Section: Resultsmentioning

confidence: 78%

Riluzole reverses blood-testis barrier loss to rescue chemotherapy-induced male infertility by binding to TRPC

Huang,

Xia,

Lin

et al. 2024

Preprint

View full text Add to dashboard Cite

Cancer treatments, including cytotoxic therapy, often result in male infertility, necessitating the development of safe and effective strategies to preserve male reproductive potential during chemotherapy. Notably, our study uncovers the potential of repurposing riluzole, an FDA-approved drug for amyotrophic lateral sclerosis (ALS), in enhancing spermatogenesis. Hence, this research aims to explore the feasibility of utilizing riluzole to alleviate male infertility induced by busulfan (BSF), a commonly used chemotherapy drug. We established a BSF-induced oligospermia model in 4-week-old male mice and found that riluzole could effectively countered the detrimental effects of BSF on sperm production in mice with oligospermia. By restoring blood-testis barrier (BTB) functionality, riluzole improves sperm quality and reduces testicular atrophy. Through transcriptomic and molecular docking analyses, we identify transient receptor potential canonical subfamily member 5 (TRPC5) as a potential target for riluzole-mediated regulation of blood-testis barrier function. These findings propose riluzole as a promising therapeutic option for chemotherapy-induced male infertility, thereby addressing the fertility challenges associated with cancer treatments. Moreover, repurposing riluzole could streamline the drug development process, providing a cost-effective approach with reduced risk compared to developing entirely new drugs.

show abstract

Section: Resultsmentioning

confidence: 78%

Riluzole reverses blood-testis barrier loss to rescue chemotherapy-induced male infertility by binding to TRPC

Huang,

Xia,

Lin

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Infertility affects approximately 30% of males and 70% of females [11]. Various problems, such as ovulatory (25%), tubular (15%), and uterine (5%) factors, cause FI, and 25% of cases remain unexplained [12]. PCOS is a major factor contributing to the development of FI [13].…”

Section: Introductionmentioning

confidence: 99%

Molecular Role of Asn680Ser and Asp37Glu Missense Variants in Saudi Women with Female Infertility and Polycystic Ovarian Syndrome

Alshammary

Alsobaie

Alageel

et al. 2023

CIMB

View full text Add to dashboard Cite

Female infertility (FI) is a global health issue. Polycystic ovary syndrome (PCOS) is a common cause of FI. The renalase gene (RNLS) is associated with FI and other human diseases. Based on the documented missense variants, rs6166 and rs2296545 single-nucleotide polymorphisms (SNPs) were not identified in Saudi women with FI and PCOS. This study aimed to investigate the molecular role of the two SNPs in Saudi women with FI and PCOS. In this cross-sectional study, 96 healthy controls, 96 people with FI, and 96 people with PCOS were recruited. DNA was isolated, and polymerase chain reactions and Sanger sequencing were performed using rs6166 and rs2296545 SNPs. The data obtained from the three groups were used to perform statistical analyses based on genotype, allele frequencies, regression models, and ANOVA. Both rs6166 and rs2296545 had no role in FI or PCOS in Saudi women. A predicted reason for non-association in Saudi women could be the role of elderly women in the controls compared with women with FI and PCOS. Moreover, age, weight, and body mass index were higher in the control group than the FI and PCOS groups. In conclusion, rs6166 and rs2296545 SNPs were not associated with FI or PCOS in Saudi women.

show abstract

The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice

Rahbar,

Asadpour,

Mazaheri

2024

J Assist Reprod Genet

View full text Add to dashboard Cite

The Role of Promyelocytic Leukemia Zinc Finger (PLZF) and Glial-Derived Neurotrophic Factor Family Receptor Alpha 1 (GFRα1) in the Cryopreservation of Spermatogonia Stem Cells

Cited by 5 publications

References 220 publications

Riluzole reverses blood-testis barrier loss to rescue chemotherapy-induced male infertility by binding to TRPC

Riluzole reverses blood-testis barrier loss to rescue chemotherapy-induced male infertility by binding to TRPC

Molecular Role of Asn680Ser and Asp37Glu Missense Variants in Saudi Women with Female Infertility and Polycystic Ovarian Syndrome

The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice

Contact Info

Product

Resources

About