The role of platelets in hemostasis and the effects of snake venom toxins on platelet function

Queiroz, Mayara Ribeiro de; Sousa, Bruna Barbosa de; Pereira, Déborah Fernanda da Cunha; Mamede, Carla Cristine Neves; Matias, Mariana Santos; Morais, Nadia Cristina Gomes de; Costa, Júnia de Oliveira; Oliveira, Fábio de

doi:10.1016/j.toxicon.2017.04.013

Cited by 54 publications

(39 citation statements)

References 192 publications

(183 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…venom. Ammodytins (Atn), acidic sPLA 2 s from the V. a. ammodytes venom, induce anticoagulant and antiplatelet effects by hydrolysing phospholipids [32]. AtnI 1 and AtnI 2 are present in the V. ursinii ssp.…”

Section: Discussionmentioning

confidence: 99%

Biological Activities and Proteomic Profile of the Venom of Vipera ursinii ssp., a very Rare Karst Viper from Croatia

Balija

Leonardi

Brgles

et al. 2020

Toxins

View full text Add to dashboard Cite

The karst viper (Vipera ursinii ssp.) favours high-mountain dry grasslands in southern and south-eastern Croatia. It is medically less important than other Vipera species, because of its remote habitat and the very small amount of venom that it injects by its relatively short fangs. The scientific literature on Vipera ursinii deals mostly with the morphology, ecology and distribution range of this snake, due to the species’ conservation issues, while the toxinological aspects of its venom have not so far been investigated. Here we report on the composition and biological activity of the Vipera ursinii ssp. venom. Using a proteomics approach, we have identified 25 proteins in the venom that belong to seven protein families: snake venom metalloproteinase, serine protease, secreted phospholipase A2, cysteine-rich secretory protein, snake C-type lectin-like protein, serine protease inhibitor and nerve growth factor. The Vipera ursinii ssp. venom was found to be distinctively insecticidal. Its lethal toxicity towards crickets was more than five times greater than that of Vipera ammodytes ammodytes venom, while the opposite held in mice. Interestingly, the mode of dying after injecting a mouse with Vipera ursinii ssp. venom may suggest the presence of a neurotoxic component. Neurotoxic effects of European vipers have so far been ascribed exclusively to ammodytoxins and ammodytoxin-like basic secreted phospholipases A2. Structural and immunological analyses of the Vipera ursinii ssp. venom, however, confirmed that ammodytoxin-like proteins are not present in this venom.

show abstract

Section: Discussionmentioning

confidence: 99%

Biological Activities and Proteomic Profile of the Venom of Vipera ursinii ssp., a very Rare Karst Viper from Croatia

Balija

Leonardi

Brgles

et al. 2020

Toxins

View full text Add to dashboard Cite

show abstract

“…Two examples of proteases are metalloproteases, which are involved in the hemorrhagic process, edema, inflammation, and necrosis, and serine proteases, which act selectively on coagulation cascade factors, affecting platelet aggregation, fibrinolysis, and coagulation. [25] Blood coagulation is a physiological process that under normal conditions occurs by the activation of proteins and cellular components involved in a sequence of reactions, which ultimately results in degradation of fibrinogen by thrombin and forms fibrin molecules. [26] Therefore, any disorder that occurs in the mechanisms controlling the hemostatic system, such as excessive coagulation, may contribute to the development of cardiovascular diseases (e. g. heart attack), stroke, and pulmonary embolism.…”

Section: Coagulant/anticoagulant Activitymentioning

confidence: 99%

Prospection of Enzyme Modulators in Aqueous and Ethanolic Extracts of Lippia sidoides Leaves: Genotoxicity, Digestion, Inflammation, and Hemostasis

Braga

Abreu

Trento

et al. 2019

Chemistry & Biodiversity

View full text Add to dashboard Cite

The aqueous and ethanolic extracts of Lippia sidoides Cham. were chemically characterized and tested for their action on enzymes involved in processes such as inflammation, blood coagulation, and digestion. Both extracts potentiated the activity of phospholipases A2 present in the venom of Bothrops atrox in 12 % and completely inhibited the hemolysis induced by B. jararacussu and B. moojeni venoms in the proportions between 1 : 0.5 and 1 : 5 (venom/extracts (w/w)). They inhibited the thrombolysis induced by B. moojeni (10 to 25 %), potentiated the thrombolysis induced by the Lachesis muta muta venom (30 to 80 %), prolonged the coagulation time induced by B. moojeni and L. muta muta venoms, and presented antigenotoxic action. Both extracts reduced the activity of α‐glycosidases, the aqueous extract inhibited lipases, and the ethanolic extract inhibited α‐amylases. The results demonstrate the modulatory action of the extracts on proteases, phospholipases, and digestive enzymes. In addition, the rich phenolic composition of these extracts highlights their potential for nutraceutical use.

show abstract

“…Platelets are cytoplasmic fragments (1 to 4 μm in diameter) produced as a result of fragmentation from megakaryocytes that are cells from bone marrow. Platelets are non-nucleated organelles that have functional characteristics like complete cell, since they possess cytoskeleton, mitochondria, Golgi residues, and endoplasmic reticulum involved in the synthesis of enzymes, storage of calcium ions, as well as storage granules [118,119]. These storage granules are δ-granules [120], α-granules, and lysosomal granules [121], which play an important role in homeostasis, inflammation, wound healing, and cell-matrix interactions.…”

Section: Plateletsmentioning

confidence: 99%

Physiology and Pathology of Innate Immune Response Against Pathogens

Muñoz‐Carrillo¹,

García²,

Coronado³

et al. 2017

Physiology and Pathology of Immunology

View full text Add to dashboard Cite

Pathogen infections are recognized by the immune system, which consists of two types of responses: an innate immune response and an antigen-specific adaptive immune response. The innate response is characterized by being the first line of defense that occurs rapidly in which leukocytes such as neutrophils, monocytes, macrophages, eosinophils, mast cells, dendritic cells, etc., are involved. These cells recognize the pathogen-associated molecular patterns (PAMPs), which have been evolutionarily conserved by the diversity of microorganisms that infect humans. Recognition of these pathogen-associated molecular patterns occurs through pattern recognition receptors such as Toll-like receptors and some other intracellular receptors such as nucleotide oligomerization domain (NOD), with the aim of amplifying the inflammation and activating the adaptive cellular immune response, through the antigenic presentation. In the present chapter, we will review the importance of the main components involved in the innate immune response, such as different cell types, inflammatory response, soluble immune mediators and effector mechanisms exerted by the immune response against bacteria, viruses, fungi, and parasites; all with the purpose of eliminating them and eradicating the infection of the host.

show abstract

The role of platelets in hemostasis and the effects of snake venom toxins on platelet function

Cited by 54 publications

References 192 publications

Biological Activities and Proteomic Profile of the Venom of Vipera ursinii ssp., a very Rare Karst Viper from Croatia

Biological Activities and Proteomic Profile of the Venom of Vipera ursinii ssp., a very Rare Karst Viper from Croatia

Prospection of Enzyme Modulators in Aqueous and Ethanolic Extracts of Lippia sidoides Leaves: Genotoxicity, Digestion, Inflammation, and Hemostasis

Physiology and Pathology of Innate Immune Response Against Pathogens

Contact Info

Product

Resources

About