The Role of Oxidative Stress and Natural Antioxidants in Ovarian Aging

Yang, Liuqing; Chen, Yun; Liu, Yan; Yu, Xiuping; Miao, Chenyun; Zhao, Ying; Chang, Xiangwei; Zhang, Qin

doi:10.3389/fphar.2020.617843

Cited by 101 publications

(58 citation statements)

References 228 publications

(252 reference statements)

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“…Oxidative stress injury of granulosa cells can lead to imbalanced follicular microenvironment, reduced function of granulosa cells, and ovarian aging [21] [22]. In this study, we found that LC can effectively alleviate the generation of excess oxygen free radicals induced by H 2 O 2 and maintain the redox balance.…”

Section: Discussionmentioning

confidence: 58%

Protective Effect of L-carnitine on the Oxidative Stress Injury of Human Ovarian Granulosa Cells

Zhang

et al. 2021

Preprint

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The protective effect of L-carnitine (LC) on the oxidative stress (OS) injury and the effect of L-carnitine on follicular stimulating hormone receptor (FSHR) of ovarian granulosa cells (GCs) were investigated. OS was induced by treatment with H2O2. We cultured KGN cells in four groups: the blank group, OS group and two L-carnitine pretreatment group (low, high). In the OS group, cell nuclear pyknosis was observed, mitochondria swelled irregularly and their cristae were fractured. Meanwhile, the cell viability, superoxide dismutase (SOD) and glutathione (GSH) contents, mitochondrial membrane potential (ΔΨm) and the level of FSHR expression were significantly decreased in the OS group. However, malonaldehyde (MDA) content, reactive oxygen species (ROS) level and apoptosis rate were significantly increased. Compared with the OS group, the morphology of cells and mitochondria in the L-carnitine pretreatment group were improved, the cell viability and the expression of FSHR was significantly increased, and the OS level was decreased. These results indicated that L-carnitine can protect the cells from OS damage induced by H2O2, enhance the antioxidant and anti-apoptotic ability of GCs, and alleviate the decrease of FSHR expression on GCs caused by OS. Therefore, L-carnitine may help prevent the ovarian aging and improve the quality of follicles.

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Section: Discussionmentioning

confidence: 58%

Protective Effect of L-carnitine on the Oxidative Stress Injury of Human Ovarian Granulosa Cells

Zhang

et al. 2021

Preprint

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“…Then the synthesis of ATP decreased, resulting in cell cycle stagnation and even apoptosis. Mitochondria participates in the whole process of follicle development [ 12 , 13 ]. De Bruin et al found that mitochondrial fraction decreased and mitochondrial matrix density increased in the 38–45 age group [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Yu Linzhu on ovarian function and oocyte mitochondria in natural aging mice

Zhang

Wei

Dong

2021

Aging

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Objective: To study the effect of Yu Linzhu on ovarian function and mitochondria in natural aging mice. Methods: Female BALB/c mice were selected as normal group at 7–8 weeks and natural aging group at 9 months. The natural aging group was divided into Yu Linzhu intervention group and non-intervention group by intragastric administration once a day for 6 weeks. The morphology and blood flow of ovary were observed by ultrasound. Ovarian morphology and follicle were observed by HE staining. Hormone levels were analyzed by ELISA. Serum oxidative stress were detected by radioimmunoassay. The distribution of mitochondria in oocytes was observed by fluorescence staining. The ultrastructure of oocytes and the morphology of mitochondria were observed under electron microscope. The mitochondrial membrane potential was detected by JC-1. Results: Two groups of aging mice had serious disturbance of estrus cycle. The ovarian area of the mice in the aging non-intervention group was smaller than that in the normal group, and the ovarian area of the mice in the aging intervention group recovered. The ovarian blood flow was weak or even disappear in the aging non-intervention group, and the blood flow in the intervention group was improved. The ovarian volume of mice in the non-intervention group was smaller than that in the normal group. Some ovarian tissues were adhered to the surrounding tissues. While in the intervention group, the ovarian volume increased, the degree of adhesion decreased, the infiltration of ovarian interstitial lymphocytes decreased, and the zona pellucida recovered. Granular cell arrangement returned neatly, egg cell shape recover regular and the number also increased. In the non-intervention group, E2 (Estrogen), AMH (Anti-Mullerian hormone) decreased ( P = 0.0092 and P = 0.0334, respectively), FSH (Follicle stimulating hormone) increased ( P < 0.0001). In the intervention group, FSH decreased ( P = 0.0002), LH (luteinizing hormone) decreased and E2, AMH increased. In the non-intervention group, GSH-Px (Glutathione peroxidase) decreased ( P = 0.0129), SOD (Superoxide dismutase) decreased, ROS (reactive oxidative species), MDA (Malondialdehyde) increased. In the aging intervention group, ROS, MDA decreased and GSH-Px increased. In the non-intervention group, mitochondrial expression was scattered at the concentrated distribution point, the length of mitochondria was mostly long and the average volume increased, the density decreased, the number decreased and some mitochondria fused, and lesions such as swelling, vacuolar degeneration and inclusion body formation, membrane potential decreased ( P = 0.0002). In the aging intervention group, mitochondria were evenly distributed, the mitochondria were basically round, the distribution density was moderate, the inner ridge was clear, and the membrane potential of the aging intervention group incre...

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“…This results in age-related fecundity decline in human and diverse animals [ 36 ]. Curcumin showed a protective effect in the ovaries involving multiple mechanisms [ 37 ]. The specific effects and mechanisms involved the following mechanisms: (1) alleviating ovarian oxidative injury, increasing nuclear factor-erythroid-2-related factor 2 (Nrf2), heme oxygenase-1(HO-1), SOD, and SOD1 levels while reducing ROS production and MDA levels; (2) decreasing the levels of caspase-3 and -9; and (3) as an anti-inflammatory agent, reducing the inflammatory markers levels such as CRP, TNF-α, and IL-6.…”

Section: Antioxidant Role Of Curcuminmentioning

confidence: 99%

“…The specific effects and mechanisms involved the following mechanisms: (1) alleviating ovarian oxidative injury, increasing nuclear factor-erythroid-2-related factor 2 (Nrf2), heme oxygenase-1(HO-1), SOD, and SOD1 levels while reducing ROS production and MDA levels; (2) decreasing the levels of caspase-3 and -9; and (3) as an anti-inflammatory agent, reducing the inflammatory markers levels such as CRP, TNF-α, and IL-6. These findings suggest that curcumin as oxidative stress modulator may represent a therapeutic intervention for delaying ovarian aging [ 37 , 38 , 39 , 40 , 41 , 42 ].…”

Section: Antioxidant Role Of Curcuminmentioning

confidence: 99%

Curcumin as Prospective Anti-Aging Natural Compound: Focus on Brain

et al. 2021

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The nutrients and their potential benefits are a new field of study in modern medicine for their positive impact on health. Curcumin, the yellow polyphenolic compound extracted from Curcuma longa species, is widely used in traditional Ayurvedic medicine to prevent and contrast many diseases, considering its antioxidant, immunomodulatory, anti-inflammatory, anti-microbial, cardio-protective, nephron-protective, hepato-protective, anti-neoplastic, and anti-rheumatic proprieties. In recent years, the investigations of curcumin have been focused on its application to aging and age-associated diseases. Aging is a physiological process in which there is a decreasing of cellular function due to internal or external stimuli. Oxidative stress is one of the most important causes of aging and age-related diseases. Moreover, many age-related disorders such as cancer, neuroinflammation, and infections are due to a low-grade chronic systemic inflammation. Curcumin acting on different proteins is able to contrast both oxidative stress than inflammation. In the brain, curcumin is able to modulate inflammation induced by microglia. Finally in brain tumors curcumin is able to reduce tumor growth by inhibition of telomerase activity. This review emphasizes the anti-aging role of curcumin focusing on its mechanism to counteract aging in the brain. Moreover, new formulations to increase the bioavailability of curcumin are discussed.

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The Role of Oxidative Stress and Natural Antioxidants in Ovarian Aging

Cited by 101 publications

References 228 publications

Protective Effect of L-carnitine on the Oxidative Stress Injury of Human Ovarian Granulosa Cells

Protective Effect of L-carnitine on the Oxidative Stress Injury of Human Ovarian Granulosa Cells

Effects of Yu Linzhu on ovarian function and oocyte mitochondria in natural aging mice

Curcumin as Prospective Anti-Aging Natural Compound: Focus on Brain

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