The role of Osteopontin in tumor metastasis

Wai, Philip Y.; Kuo, Paul C.

doi:10.1016/j.jss.2004.03.028

Cited by 335 publications

(312 citation statements)

References 180 publications

Supporting

Mentioning

296

Contrasting

Unclassified

Order By: Relevance

“…The interaction appears to be RGD-independent and to require the presence of β1 integrins (Katagiri et al, 1999). The ligation of OPN and CD44 variants mediates chemotaxis and adhesion of fibroblasts, T cells and bone marrow cells (Weber et al, 1996;Wai and Kuo, 2004). CD44 engagement with OPN down-regulates IL-10 expression in peritoneal macrophages (Ashkar et al, 2000).…”

Section: Cd44mentioning

confidence: 99%

Osteopontin: Role in immune regulation and stress responses

Wang¹,

Denhardt²

2008

Cytokine & Growth Factor Reviews

605

558

View full text Add to dashboard Cite

Section: Cd44mentioning

confidence: 99%

Osteopontin: Role in immune regulation and stress responses

Wang¹,

Denhardt²

2008

Cytokine & Growth Factor Reviews

605

558

View full text Add to dashboard Cite

“…to various integrins (avb3, avb1, avb5, a5b1, a9b1 and a4b1), and there is a thrombin cleavage site adjacent to the possible integrin-binding site (Figure 6b). 8 A comparison shows that Opn has several functions important for tumor progression and metastasis, which are nearly identical in human and rat Opn.…”

Section: Discussionmentioning

confidence: 99%

“…These proteins are important players in cell-cell and cell-matrix communications. 7 Human OPN has a size of 314 amino acids, [8][9][10] but is detected in bands with apparent sizes of 41-75 kDa. This is due to different splice variants and intensive post-translational modifications.…”

Section: Introductionmentioning

confidence: 99%

“…12,13 In line with this, Opn has been identified as lead marker of colon cancer progression, with the highest mRNA expression levels found in liver metastasis. 14 OPN has several domains including a conserved thrombin cleavage site (amino acids R168-S169 in human OPN), 8 which splits the protein into two functional fragments. The glycine-arginine-glycine-aspartate-serine (GRGDS) sequence in the N-terminal region contains an arginine-glycine-aspartate binding motif that plays an important role for interaction with various integrins (avb3, ab5, avb1, a4b1, a8b1 and a9b1) and the C-terminal fragment can bind to CD44 splice variants, thus mediating cell adhesion and migration functions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of osteopontin expression on the metastatic growth of CC531 rat colorectal carcinoma cells in rat liver

et al. 2011

View full text Add to dashboard Cite

Development of hepatic metastasis is responsible for most of colorectal cancer-related deaths. Osteopontin (OPN) is a small integrin-binding N-linked glycoprotein, which plays a crucial role in the formation of hepatic metastasis. This study aimed to suppress Opn expression by an antisense-oligonucleotide (ASO Opn ) to decrease liver metastasis in vivo. The effect of ASO Opn was investigated in vitro in CC531 lacZ colorectal cancer cells in comparison to sense (SO) or nonsense (NSO) oligomers, by determining mRNA and protein expression levels, as well as cell survival. For in vivo treatment, CC531 lacZ cells were intraportally inoculated into rats to compare the effects of ASO, SO and NSO oligomers, following prolonged subcutaneous administration by osmotic minipumps. The resulting CC531 lacZ tumor cell load in the liver was measured by a b-galactosidase assay. Proliferation of CC531 lacZ cells in vitro was significantly decreased after ASO Opn and SO treatment (Po0.001). Liver metastasis development was reduced as long as ASO Opn was administered, but this effect was rapidly blunted following the end of the ASO Opn administration. In contrast, administration of the SO resulted in a tumor load reduction, which surprisingly surpassed the ASO Opn effect in vivo in terms of a long-lasting metastasis suppression, which was accompanied with increased survival of the animals. Administration of the ASO Opn in rats was effective in decreasing their liver metastasis. The short-lived effect might be extended by modifications suited to increase the ASOs' half-life. In addition, there was a superior anti-metastatic effect caused by the SO, which has not been reported previously.

show abstract

“…High OPN levels are associated with poorer outcomes in patients with breast cancer metastasis [106] and have been shown to promote metastasis in prostate [107] and mammary carcinoma [108,109]. OPN regulates homing and invasion of tumor cells by binding with integrin and CD44 receptors [110]. Interestingly OPN has been implicated as an important regulator of migration of HSCs through the marrow and functionality of the HSC niche.…”

Section: Chemotaxis Towards Bonementioning

confidence: 99%

Homing of Cancer Cells to the Bone

Mishra

Shiozawa

Pienta

et al. 2011

Cancer Microenvironment

View full text Add to dashboard Cite

A variety of tumor cells preferentially home to the bone. The homing of cancer cells to the bone represents a multi-step process that involves malignant progression of the tumor, invasion of the tumor through the extracellular matrix and the blood vessels and settling of the tumor cells in the bone. Gaining a greater understanding as to the mechanisms used by cancer cells in these processes will facilitate the design of drugs which could specifically target the homing process. In this review we will discuss the properties of tumor cells and the bone microenvironment which promote homing of a cancer cell to the bone. We will highlight the different steps and the molecular pathways involved when a cancer cell metastasize to the bone. Since bone is the major home for hematopoietic stem cells (HSCs), we will also highlight the similarities between the homing of cancer and HSC to the bone. Finally we will conclude with therapeutic and early detection strategies which can prevent homing of a cancer cell to the bone.

show abstract

The role of Osteopontin in tumor metastasis

Cited by 335 publications

References 180 publications

Osteopontin: Role in immune regulation and stress responses

Osteopontin: Role in immune regulation and stress responses

Influence of osteopontin expression on the metastatic growth of CC531 rat colorectal carcinoma cells in rat liver

Homing of Cancer Cells to the Bone

Contact Info

Product

Resources

About