The role of osmotic stress and surface energy in freezing injury of sea urchin eggs

Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin ( Paracentrotus lividus ) oocytes

“…It is possible that both the fitted constant, C osm and the fitted power, β , are related to temperature via the Arrhenius relation, as we found with cytotoxicity damage. Indeed, Wang et al (2011) found accumulated osmotic damage was temperature specific while Williams & Takahashi (1982) found membrane lysis of sea urchin eggs was mitigated at lower temperatures during hypertonic challenge. Supplements that prevent membrane and cytoskeletal regulation may reduce time-dependent osmotic damage ( Asadi, Najafi & Benson, 2022 ).…”

Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin ( Paracentrotus lividus ) oocytes

“…It is possible that both the fitted constant, C osm and the fitted power, β , are related to temperature via the Arrhenius relation, as we found with cytotoxicity damage. Indeed, Wang et al (2011) found accumulated osmotic damage was temperature specific while Williams & Takahashi (1982) found membrane lysis of sea urchin eggs was mitigated at lower temperatures during hypertonic challenge. Supplements that prevent membrane and cytoskeletal regulation may reduce time-dependent osmotic damage ( Asadi, Najafi & Benson, 2022 ).…”

Rational synthesis of total damage during vitrification: modelling and experimental validation of osmotic, temperature, and cytotoxic damage in sea urchin ( Paracentrotus lividus) oocytes

“…It is possible that both the fitted constant, C osmo and the fitted power, β , are related to temperature via the Arrhenius relation, as we found with cytotoxicity damage. Indeed, Wang and colleagues (26) found accumulated osmotic damage was temperature specific while Williams and Takahashi (64) found membrane lysis of sea urchin eggs was mitigated at lower temperatures during hypertonic challenge. Supplements that prevent membrane and cytoskeletal regulation may reduce time dependent osmotic damage.…”

Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin ( Paracentrotus lividus ) oocytes

“…It is possible that both the fitted constant, C osm and the fitted power, β , are related to temperature via the Arrhenius relation, as we found with cytotoxicity damage. Indeed, Wang et al (2011) found accumulated osmotic damage was temperature specific while Williams & Takahashi (1982) found membrane lysis of sea urchin eggs was mitigated at lower temperatures during hypertonic challenge. Supplements that prevent membrane and cytoskeletal regulation may reduce time-dependent osmotic damage ( Asadi, Najafi & Benson, 2022 ).…”

Rational synthesis of total damage during vitrification: modelling and experimental validation of osmotic, temperature, and cytotoxic damage in sea urchin ( Paracentrotus lividus) oocytes

“…It is possible that both the fitted constant, C osmo and the fitted power, β , are related to temperature via the Arrhenius relation, as we found with cytotoxicity damage. Indeed, Wang and colleagues (26) found accumulated osmotic damage was temperature specific while Williams and Takahashi (64) found membrane lysis of sea urchin eggs was mitigated at lower temperatures during hypertonic challenge. Supplements that prevent membrane and cytoskeletal regulation may reduce time dependent osmotic damage.…”

Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin ( Paracentrotus lividus ) oocytes

“…It is possible that both the fitted constant, C osm and the fitted power, β , are related to temperature via the Arrhenius relation, as we found with cytotoxicity damage. Indeed, Wang et al (2011) found accumulated osmotic damage was temperature specific while Williams & Takahashi (1982) found membrane lysis of sea urchin eggs was mitigated at lower temperatures during hypertonic challenge. Supplements that prevent membrane and cytoskeletal regulation may reduce time-dependent osmotic damage ( Asadi, Najafi & Benson, 2022 ).…”

Rational synthesis of total damage during vitrification: modelling and experimental validation of osmotic, temperature, and cytotoxic damage in sea urchin ( Paracentrotus lividus) oocytes

“…It is possible that both the fitted constant, C osmo and the fitted power, β , are related to temperature via the Arrhenius relation, as we found with cytotoxicity damage. Indeed, Wang and colleagues (26) found accumulated osmotic damage was temperature specific while Williams and Takahashi (64) found membrane lysis of sea urchin eggs was mitigated at lower temperatures during hypertonic challenge. Supplements that prevent membrane and cytoskeletal regulation may reduce time dependent osmotic damage.…”