The role of nitric oxide in the pathogenesis of preeclampsia

Seligman, Susan P.; Buyon, Jill P.; Clancy, Robert M.; Young, Bruce K.; Abramson, Steven B.

doi:10.1016/s0002-9378(94)70064-8

Cited by 370 publications

(201 citation statements)

References 18 publications

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“…At the same time, fetal growth restriction may be also consequence of the increased oxidative stress in those women with preeclampsia; this has also been suggested in other studies, such as Fujimaki et al 31 Regarding nitric oxide levels in preeclampsia or in other hypertensive disorders, the results of different studies are contradictory; both elevated and diminished levels have been found. 32,33 We found a significant increment in nitrate levels in preeclampsia and similar levels in the other hypertensive groups when compared with PCW.…”

Section: Discussionsupporting

confidence: 66%

Abnormal levels of antioxidant defenses in a large sample of patients with hypertensive disorders of pregnancy

et al. 2011

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Increased levels of oxidative stress have been demonstrated in Preeclampsia in previous studies, but this finding has not been established in other hypertensive disorders in pregnancy (HDP). We measured different markers of lipid peroxidation and antioxidant defenses by spectrophotometry or enzymoimmunoanalysis in 339 pregnant women: 85 with gestational hypertension (GH), 88 chronic hypertension (CH), 104 Preeclampsia and 62 healthy pregnant control women (PCW). Lower activity of superoxide dismutase and higher levels of catalase were found in GH, CH and preeclampsia compared with PCW (964.4±116.5, 970.0±120.4, 971.2±137.5 and 1063.4±133.7 U g À1 Hb, Po0.001; and 313.0±71.7, 292.2±45.3, 297.1 ± 47.2, 215.5 ± 26.2 U mg À1 Hb, Po0.0001; respectively). Regarding the glutathione REDOX cycle, we found the following in GH, CH and preeclampsia compared with PCW: a decrease in its reduced form (2.6 ± 0.6, 2.7 ± 0.8, 2.7 ± 0.9, 3.3±1.3 lmol l À1 , Po0.003), a parallel increase in the oxidized form (185.6±68.9, 194.7±75.0, 184.3±78.3, 85.1±27.5 lmol l À1 , Po0.0001) and an increment in glutathione peroxidase (85.9±22.0, 86.4±20.9, 82.1±23.5 and 77.2 ± 19.7 U g À1 Hb, Po0.04) and glutathione reductase (6384.3 ± 1261.9, 6724.6 ± 1154.1, 6287.9 ± 1399.9 and 6044.4±1208.4 mU g À1 Hb, Po0.01, respectively). Nitrites/nitrates were higher in patients with preeclampsia than in PCW (31.50±15.08, 26.80±8.39 lmol l À1 , Po0.002). Although malondialdehyde and oxidized-LDL levels were similar among groups, free fatty acids were increased in every HDP (GH 514.6 ± 194.6, CH 501.3 ± 197.4, preeclampsia 555.2 ± 230.1 lmol l À1 ) compared with PCW (351.4 ± 146.1 lmol l À1 ), Po0.0001. Our results show an oxidation/reduction imbalance with an increase in oxidative stress coupled with a decreased capacity of antioxidant systems, not only in preeclampsia but also in every HDP.

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Section: Discussionsupporting

confidence: 66%

Abnormal levels of antioxidant defenses in a large sample of patients with hypertensive disorders of pregnancy

et al. 2011

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“…However, TNF-is known to change endothelial cells from an antihaemostatic to a procoagulant state by inducing the expression of tissue factor and by modulating other cellular functions [33,34]. Fourth, decreased levels of nitric oxide, a potent vasodilator released by endothelial cells, have been found in pre-eclamptic patients [35]. TNF-has an inhibiting effect on nitric oxide synthesis [36].…”

Section: Discussionmentioning

confidence: 99%

Tumour necrosis factor-alpha (TNF-α) gene polymorphism and expression in pre-eclampsia

Chen

Wilson²,

Wang

et al. 1996

Clinical and Experimental Immunology

156

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SUMMARYPre-eclampsia is an endothelial disorder and TNF-has fundamental effects on endothelial cells by several means, including altering the balance between oxidant and anti-oxidant, changing the pattern of prostaglandin production and affecting expression of several cell surface components. To determine whether TNF-mRNA expression is increased in pre-eclamptic patients, leucocytes from pre-eclamptic patients, normal pregnant women and normal non-pregnant women were studied for TNF-expression using a quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. Using a model of 373A ABI Sequencer, TNF-gene polymorphism was also analysed by GENESCAN and Genotyper software in order to explain the mechanism of abnormal TNF-expression. Our results show that TNFmRNA expression is significantly elevated in pre-eclamptic patients compared with the other two control groups. The high expression of TNF-may be associated with the TNF1 allele, whose frequency is markedly increased in pre-eclamptic patients. These observations are consistent with a major role for TNF-in mediating endothelial disturbances, and suggest a key role for TNF-in the development of pre-eclampsia.

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“…The mean values determined for the reaction rate constants in the degradation of 7V°-nitro-L-arginine methyl ester and its A/°-niuO-L-arginine are confirmed to be suitable for calculating the concentrations of one or both substances at the time of sampling in the first approximation using equations [6] and [7], derived from [1] and [2]. Equation [7] is only required for calculating N°-nitro-L-arginine if both substances are present in a sample.…”

Section: Resultsmentioning

confidence: 81%

“…Previous studies have shown that there is an impotant relationship between nitric oxide and the regulation of blood pressure during pregnancy. In pathological cases, changes have been demonstrated in the concentrations of nitrite and nitrate (6,7). In patients with preeclampsia, for example, the concentration of nitrite in the blood is reduced.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of the Degradation of N ^GNitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

Klein¹,

Dudenhausen²

1996

CCLM

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Summary:The kinetics of the degradation of the inhibitors of the nitric oxide synthesis, A/°-nitro-L-arginine methyl ester and A^-nitro-Zx-arginine, were examined in human amniotic fluid and umbilical vein blood. The reaction rate constants were calculated or estimated using the time-controlled concentration course of both substances. These concentrations were measured by high-performance liquid chromatography with two different separation systems: ion-exchange chromatography and ion-pair chromatography. Using this method, either A/°-nitro-L-arginine methyl ester and/or M^nitro-L-arginine were added to 18 samples of amniotic fluid, 33 samples of plasma and 21 samples of uncentrifuged umbilical vein blood samples; subsequently these samples were used for measurement. The degradation of the two individual study substances can be described by a uni-unimolecular two-step consecutive reaction. Thereby, M'-nitro-L-arginine methyl ester decomposes to A/°-nitro-L-arginine. Although 7V°-nitro-£-arginine decomposed further, the decomposition product could not be identified. The average of the reaction rate constants for A/°-nitro-L-arginine methyl ester/7V G -nitro-L-arginine was determined, yielding the following values: 0.032 h~V 0.00047h-1 in amniotic fluid, 0.029 rrVO.00384 h" 1 and 0.00074h" 1 in plasma and O.SOlrVO.OOOOO IT 1 in uncentrifuged umbilical vein blood. During the first hours after sampling, these reaction rate constants could be used to approximate the concentrations of M^-nitro-L-arginine methyl ester and 7V°-nitro-L-arginine at the time of sampling.

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The role of nitric oxide in the pathogenesis of preeclampsia

Cited by 370 publications

References 18 publications

Abnormal levels of antioxidant defenses in a large sample of patients with hypertensive disorders of pregnancy

Abnormal levels of antioxidant defenses in a large sample of patients with hypertensive disorders of pregnancy

Tumour necrosis factor-alpha (TNF-α) gene polymorphism and expression in pre-eclampsia

Kinetics of the Degradation of N ^GNitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

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The role of nitric oxide in the pathogenesis of preeclampsia

Cited by 370 publications

References 18 publications

Abnormal levels of antioxidant defenses in a large sample of patients with hypertensive disorders of pregnancy

Abnormal levels of antioxidant defenses in a large sample of patients with hypertensive disorders of pregnancy

Tumour necrosis factor-alpha (TNF-α) gene polymorphism and expression in pre-eclampsia

Kinetics of the Degradation of N G Nitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

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Kinetics of the Degradation of N ^GNitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid