The role of neutrophils in asthma

Chen, Fei; Yu, Min; Zhong, Yueyang; Huang, Wen; Huang, Huaqiong

doi:10.3724/zdxbyxb-2021-0030

Cited by 6 publications

(4 citation statements)

References 59 publications

(20 reference statements)

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“…In our current study, Cluster1 presented activated memory B cells, plasma cells, and monocytes, while in Cluster 2, the most remarkable immune infiltrating cells were naïve CD4 T cells, activated dendritic cells, activated mast cells, and neutrophils. Neutrophils and activated mast cells are considered key factors in the occurrence and progression of various diseases ( Levick and Widiapradja, 2018 ; Chen F. et al, 2021 ). Dendritic cells (DCs), the most important antigen-presenting cells, play a primary role in regulating the adaptive immune response by identifying, processing, and presenting external antigens to T cells ( Song et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Ferroptosis-Related Molecular Clusters and Immune Characterization in Autism Spectrum Disorder

et al. 2022

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Introduction: Autism spectrum disorder (ASD) is a neurodevelopmental disorder with clinical presentation and prognostic heterogeneity. Ferroptosis is a regulated non-apoptotic cell death program implicated in the occurrence and progression of various diseases. Therefore, we aimed to explore ferroptosis-related molecular subtypes in ASD and further illustrate the potential mechanism.Methods: A total of 201 normal samples and 293 ASD samples were obtained from the Gene Expression Omnibus (GEO) database. We used the unsupervised clustering analysis to identify the molecular subtypes based on ferroptosis-related genes (FRGs) and evaluate the immune characteristics between ferroptosis subtypes. Ferroptosis signatures were identified using the least absolute shrinkage and selection operator regression (LASSO) and recursive feature elimination for support vector machines (SVM-RFE) machine learning algorithms. The ferroptosis scores based on seven selected genes were constructed to evaluate the ferroptosis characteristics of ASD.Results: We identified 16 differentially expressed FRGs in ASD children compared with controls. Two distinct molecular clusters associated with ferroptosis were identified in ASD. Analysis of immune infiltration revealed immune heterogeneity between the two clusters. Cluster2, characterized by a higher immune score and a larger number of infiltrated immune cells, exhibited a stronger immune response and was markedly enriched in immune response-related signaling pathways. Additionally, the ferroptosis scores model was capable of predicting ASD subtypes and immunity. Higher levels of ferroptosis scores were associated with immune activation, as seen in Cluster2. Lower ferroptosis scores were accompanied by relative immune downregulation, as seen in Cluster1.Conclusion: Our study systematically elucidated the intricate correlation between ferroptosis and ASD and provided a promising ferroptosis score model to predict the molecular clusters and immune infiltration cell profiles of children with ASD.

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Section: Discussionmentioning

confidence: 99%

Identification of Ferroptosis-Related Molecular Clusters and Immune Characterization in Autism Spectrum Disorder

et al. 2022

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“…The movement trajectory and time of the mice in the cage were automatically recorded by the soware for four days. (2) The space exploration experiment. On the 5th day of the water maze test, the xed position of the platform was withdrawn, and the mice in each group were placed in the water of the fourth quadrant of the diagonal.…”

Section: Learning and Memory Testmentioning

confidence: 99%

“…The mice were placed in the test box and removed aer 5 min of random activity. (2) The training period: aer 24 h of the adaptation period, two objects with the same shape (numbered A1 and A2) were placed in the test chamber before training. The mice were placed back in A1 and A2 in the test chamber, and the mice were removed aer 5 min of random activity.…”

Section: Learning and Memory Testmentioning

confidence: 99%

“…It is composed of seven traditional Chinese medicines (TCM): Renshen ( Radix ginseng ), Shudihuang ( Radix rehmanniae ), Danggui ( Radix angelicae ), Baizhu ( Atractylodes macrocephala ), Gancao ( Glycyrrhiza uralensis ), Suanzaoren ( Ziziphus jujuba ), and Yuanzhi ( Polygala tenuifolia ). 2,3 It tonifies the blood and replenishes qi, essence, and marrow and strengthens the spirit. Moreover, it treats qi and blood deficiency, as well as heart and spleen disorders.…”

Section: Introductionmentioning

confidence: 99%

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