The Role of Metal Nanoparticles in Remote Release of Encapsulated Materials

Skirtach, André G.; Déjugnat, Christophe; Braun, Dieter; Susha, Andrei S.; Rogach, Andrey L.; Parak, Wolfgang J.; Möhwald, Helmuth; Sukhorukov, Gleb B.

doi:10.1021/nl050693n

Cited by 525 publications

(488 citation statements)

References 38 publications

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“…When a laser pulse of sufficiently high intensity is applied, the temperature of the AuNPs increases to several hundred degrees causing the water surrounding the AuNPs to evaporate. 46 This in turn results in the formation of vapour nanobubbles that expand and collapse, thereby inducing pores in the cell membrane and allowing the contrast agent to diffuse into the cytoplasm. In our set-up, the cells are grown in a 96-well plate and the laser beam covers a circular area of ~150 µm diameter.…”

Section: Resultsmentioning

confidence: 99%

Cytosolic Delivery of Nanolabels Prevents Their Asymmetric Inheritance and Enables Extended Quantitative in Vivo Cell Imaging

et al. 2016

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Long-term in vivo imaging of cells is crucial for the understanding of cellular fate in biological processes in cancer research, immunology or in cell-based therapies such as beta cell transplantation in type I diabetes or stem cell therapy. Traditionally, cell labelling with the desired contrast agent occurs ex vivo via spontaneous endocytosis, which is a variable and slow process that requires optimization for each particular label-cell type combination. Following endocytic uptake, the contrast agents mostly remain entrapped in the endolysosomal compartment, which leads to signal instability, cytotoxicity and asymmetric inheritance of the labels upon cell division. Here, we demonstrate that these disadvantages can be circumvented by delivering contrast agents directly into the cytoplasm via vapour nanobubble photoporation. Compared to classic endocytic uptake, photoporation resulted in 50 and 3 times higher loading of fluorescent dextrans and quantum dots, respectively, with improved signal stability and reduced cytotoxicity. Most interestingly, cytosolic delivery by photoporation prevented asymmetric inheritance of labels by daughter cells over subsequent cell generations. Instead, unequal inheritance of endocytosed labels resulted in a dramatic increase in polydispersity of the amount of labels per cell with each cell division, hindering accurate quantification of cell numbers in vivo over time. The combined benefits of cell labelling by photoporation resulted in a marked improvement in long-term cell visibility in vivo where an insulin producing cell line (INS-1E cell line) labelled with fluorescent dextrans could be tracked for up to two months in Swiss Nude mice compared to two weeks for cells labelled by endocytosis.

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Section: Resultsmentioning

confidence: 99%

Cytosolic Delivery of Nanolabels Prevents Their Asymmetric Inheritance and Enables Extended Quantitative in Vivo Cell Imaging

et al. 2016

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“…Partially loaded and fully loaded capsules were observed and the presence of iron originating from the MOF cages was confirmed with energy dispersive X‐ray spectroscopy (EDX) (Figure 1). Other inorganic materials, such as gold nanoparticles of different sizes (Figure S3, Supporting Information), capable of catalysis and photothermal reactions,28 nanodiamonds (Figure S4, Supporting Information), capable of detecting ionic spin29 and useful for biomedical applications,30 and fluorescent/magnetic iron oxide nanoparticles31 (Figure S5, Supporting Information) were loaded onto the PSS–CaCO 3 particles and were capped with PAH for the preparation of functional capsules.…”

Section: Resultsmentioning

confidence: 99%

Versatile Loading of Diverse Cargo into Functional Polymer Capsules

et al. 2015

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Polymer microcapsules are of particular interest for applications including self‐healing coatings, catalysis, bioreactions, sensing, and drug delivery. The primary way that polymer capsules can exhibit functionality relevant to these diverse fields is through the incorporation of functional cargo in the capsule cavity or wall. Diverse functional and therapeutic cargo can be loaded into polymer capsules with ease using polymer‐stabilized calcium carbonate (CaCO3) particles. A variety of examples are demonstrated, including 15 types of cargo, yielding a toolbox with effectively 500+ variations. This process uses no harsh reagents and can take less than 30 min to prepare, load, coat, and form the hollow capsules. For these reasons, it is expected that the technique will play a crucial role across scientific studies in numerous fields.

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“…[128][129][130] Additionally, because of their ability to efficiently convert light into heat, gold nanoparticles are excellent for investigations of dynamic processes by manipulating these processes with controllable application of heat. 131 The membranes of living cells are extremely complex structures and many vital processes, such as cellular respiration, nutrient recognition and ion transport/signalling occur at or in cellular and subcellular membranes. Gold nanoparticles are excellent candidates for investigating some of these processes due to the aforementioned optical characteristics along with a low toxicity and extensive knowledge of modication of the gold surface.…”

Section: Manipulation Of Biomimetic and Biological Systemsmentioning

confidence: 99%

Optical trapping and manipulation of plasmonic nanoparticles: fundamentals, applications, and perspectives

et al. 2014

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This feature article discusses the optical trapping and manipulation of plasmonic nanoparticles, an area of current interest with potential applications in nanofabrication, sensing, analytics, biology and medicine. We give an overview over the basic theoretical concepts relating to optical forces, plasmon resonances and plasmonic heating. We discuss fundamental studies of plasmonic particles in optical traps and the temperature profiles around them. We place a particular emphasis on our own work employing optically trapped plasmonic nanoparticles towards nanofabrication, manipulation of biomimetic objects and sensing.

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The Role of Metal Nanoparticles in Remote Release of Encapsulated Materials

Cited by 525 publications

References 38 publications

Cytosolic Delivery of Nanolabels Prevents Their Asymmetric Inheritance and Enables Extended Quantitative in Vivo Cell Imaging

Cytosolic Delivery of Nanolabels Prevents Their Asymmetric Inheritance and Enables Extended Quantitative in Vivo Cell Imaging

Versatile Loading of Diverse Cargo into Functional Polymer Capsules

Optical trapping and manipulation of plasmonic nanoparticles: fundamentals, applications, and perspectives

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