The role of extracellular matrix in biomechanics and its impact on bioengineering of cells and 3D tissues

Urbanczyk, Max; Layland, Shannon L.; Schenke‐Layland, Katja

doi:10.1016/j.matbio.2019.11.005

Cited by 133 publications

(108 citation statements)

References 127 publications

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“…Exogenous biomechanical cues are known to impact cellular behavior. 1 Therefore, we investigated whether providing an external COL1 hydrogel changes the ECM protein secretion patterns in the pseudoislet cultures ( Fig. 5G-M).…”

Section: Col1 Hydrogel Mimics Native Pancreatic Tissue and Attenuatesmentioning

confidence: 99%

“…It consists of large fibrillar proteins, glycoproteins, and proteoglycans, as well as water, growth factors, enzymes, and other biomolecules. 1 The ECM is vital for cell survival and function as it provides biological, chemical, and mechanical cues to cells, which in turn, remodel the ECM to control tissue homeostasis. 1 Alterations and disruptions of the ECM homeostatic state adversely affect cells resulting in structural tissue damages, diseases, and potentially organ failure.…”

Section: Introductionmentioning

confidence: 99%

“…1 The ECM is vital for cell survival and function as it provides biological, chemical, and mechanical cues to cells, which in turn, remodel the ECM to control tissue homeostasis. 1 Alterations and disruptions of the ECM homeostatic state adversely affect cells resulting in structural tissue damages, diseases, and potentially organ failure. 2 Mechanical disruption of the ECM that occurs during injury or is induced during transplant surgery can severely compromise cell survival and function, ultimately hindering cell engraftment.…”

Section: Introductionmentioning

confidence: 99%

“…2 Mechanical disruption of the ECM that occurs during injury or is induced during transplant surgery can severely compromise cell survival and function, ultimately hindering cell engraftment. 1 This is a major issue in cell and tissue transplantation due to a number of underlying factors, including the hypoxic environment at the transplant site, the implant/transplanttriggered immune response, and pathological ECM remodeling posttransplantation.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Collagen and Endothelial Cell Coculture Improves β-Cell Functionality and Rescues Pancreatic Extracellular Matrix

Zbinden

Urbanczyk

Layland

et al. 2021

Tissue Engineering Part A

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The use of biomaterials and biomaterial functionalization is a promising approach to support pancreatic islet viability posttransplantation in an effort to reduce insulin dependence for patients afflicted with diabetes mellitus type 1. Extracellular matrix (ECM) proteins are known to impact numerous reparative functions in the body. Assessing how endogenously expressed pancreatic ECM proteins are affected by posttransplant-like hypoxic conditions may provide significant insights toward the development of tissue-engineered therapeutic strategies to positively influence b-cell survival, proliferation, and functionality. Here, we investigated the expression of three relevant groups of pancreatic ECM proteins in human native tissue, including basement membrane (BM) proteins (collagen type 4 [COL4], laminins [LAM]), proteoglycans (decorin [DCN], nidogen-1 [NID1]), and fibril-forming proteins (fibronectin [FN], collagen type 1 [COL1]). In an in vitro hypoxia model, we identified that ECM proteins were differently affected by hypoxic conditions, contributing to an overall loss of b-cell functionality. The use of a COL1 hydrogel as carrier material demonstrated a protective effect on bcells mitigating the effect of hypoxia on proteoglycans as well as fibril-forming protein expression, supporting b-cell functionality in hypoxia. We further showed that providing endothelial cells (ECs) into the COL1 hydrogel improves b-cell response as well as the expression of relevant BM proteins. Our data show that b-cells benefit from a microenvironment composed of structure-providing COL1 with the incorporation of ECs to withstand the harsh conditions of hypoxia. Such hydrogels support b-cell survival and can serve as an initial source of ECM proteins to allow cell engraftment while preserving cell functionality posttransplantation.

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Section: Col1 Hydrogel Mimics Native Pancreatic Tissue and Attenuatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Collagen and Endothelial Cell Coculture Improves β-Cell Functionality and Rescues Pancreatic Extracellular Matrix

Zbinden

Urbanczyk

Layland

et al. 2021

Tissue Engineering Part A

Self Cite

View full text Add to dashboard Cite

show abstract

“…Collagen, as a natural polymer, is the most abundant extracellular matrix (ECM) protein; it is present within mineralized tissues, connective fibrous tissues, and various organs [ 55 , 56 , 57 ] and provides essential structures to support cell–tissue morphogenesis [ 58 , 59 , 60 ]. The interaction between collagen matrices and cells–tissues can produce various signaling molecules to regulate cell adhesion, proliferation, differentiation, and migration [ 61 ] and physiologically promote tissue development [ 58 , 62 ]. The collagen has typical triple-helical structures of three polypeptide chains, and their supramolecular organization can be characterized by its extreme complexity and the various functions with tissue-adaptable forms [ 63 , 64 ].…”

Section: Natural Biopolymers For Periodontal Hard Tissue Regeneratmentioning

confidence: 99%

Tooth-Supporting Hard Tissue Regeneration Using Biopolymeric Material Fabrication Strategies

Kim

Park

2020

Molecules

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The mineralized tissues (alveolar bone and cementum) are the major components of periodontal tissues and play a critical role to anchor periodontal ligament (PDL) to tooth-root surfaces. The integrated multiple tissues could generate biological or physiological responses to transmitted biomechanical forces by mastication or occlusion. However, due to periodontitis or traumatic injuries, affect destruction or progressive damage of periodontal hard tissues including PDL could be affected and consequently lead to tooth loss. Conventional tissue engineering approaches have been developed to regenerate or repair periodontium but, engineered periodontal tissue formation is still challenging because there are still limitations to control spatial compartmentalization for individual tissues and provide optimal 3D constructs for tooth-supporting tissue regeneration and maturation. Here, we present the recently developed strategies to induce osteogenesis and cementogenesis by the fabrication of 3D architectures or the chemical modifications of biopolymeric materials. These techniques in tooth-supporting hard tissue engineering are highly promising to promote the periodontal regeneration and advance the interfacial tissue formation for tissue integrations of PDL fibrous connective tissue bundles (alveolar bone-to-PDL or PDL-to-cementum) for functioning restorations of the periodontal complex.

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Hydrostatic pressure induces profibrotic properties in hepatic stellate cells via the RhoA/ROCK signaling pathway

Huang

Khalifa

et al. 2022

FEBS Open Bio

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Elevated interstitial fluid hydrostatic pressure is commonly observed in diseased livers. We herein examined the hypothesis that hydrostatic pressure induces hepatic stellate cells to acquire profibrotic properties under pathological conditions. Human hepatic stellate cells were exposed to 50 mmHg pressure for 24 h. Although we observed few changes of cell growth and morphology, PCR array data on the expression of fibrosis‐associated genes suggested the acquisition of profibrotic properties. The exposure of hepatic stellate cells to 50 mmHg pressure for 24 h also significantly enhanced the expression of RhoA, ROCK1, α‐SMA, TGF‐β1, p‐MLC, and p‐Smad2, and this was effectively attenuated by the ROCK inhibitor Y‐27632. Our ex vivo experimental data suggest that elevated interstitial fluid hydrostatic pressure under pathological conditions may promote liver fibrosis by inducing acquisition of profibrotic properties of hepatic stellate cells through the RhoA/ROCK signaling pathway.

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The role of extracellular matrix in biomechanics and its impact on bioengineering of cells and 3D tissues

Cited by 133 publications

References 127 publications

Collagen and Endothelial Cell Coculture Improves β-Cell Functionality and Rescues Pancreatic Extracellular Matrix

Collagen and Endothelial Cell Coculture Improves β-Cell Functionality and Rescues Pancreatic Extracellular Matrix

Tooth-Supporting Hard Tissue Regeneration Using Biopolymeric Material Fabrication Strategies

Hydrostatic pressure induces profibrotic properties in hepatic stellate cells via the RhoA/ROCK signaling pathway

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