The role of choline in prostate cancer

Awwad, Hussain Mohamad; Geisel, Juergen; Obeid, Rima

doi:10.1016/j.clinbiochem.2012.08.012

Cited by 76 publications

(66 citation statements)

References 65 publications

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“…Notably, the SLC44A1-5 family has been shown to be functionally important in the development of human lung, prostate and colon carcinoma (17)(18)(19)(20), however, the mechanisms underlying the effects of the SLC44A1-5 family remain to be elucidated. One of these proteins, SLC44A1, has previously been found to stimulate NCI-H69 cell growth and choline uptake, suggesting that SLC44A1 may function as a lung carcinogenic gene (17).…”

Section: Discussionmentioning

confidence: 99%

Knockdown by shRNA identifies SLC44A5 as a potential therapeutic target in hepatocellular carcinoma

Peng

Ren

et al. 2016

Molecular Medicine Reports

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Abstract. Hepatocellular carcinoma (HCC) has been ranked the second leading cause of cancer-associated mortality in China and the third leading cause of cancer-associated mortality worldwide. A number of previous studies investigating SLC44A5 have revealed important biological insight and disease-specific functions. Therefore, the present study investigated the expression of SLC44A5 in HCC tissues and cell lines, and assessed the effect of SLC44A5 on the viability, cell cycle, apoptosis and invasion of HCC cell lines. The mRNA expression of SLC44A5 in 35 HCC tissues was significantly higher compared with that in 35 normal tissues. The protein expression of SLC44A5 was notably high in MHCC-97H and SMMC-7721 cells compared with that in four other HCC cell lines. Knockdown of SLC44A5 using short hairpin RNA inhibited cell viability and arrested the cells in G1 of the cell cycle by reducing the expression of cell cycle markers, proliferating cell nuclear antigen and cyclin-dependent kinase 2 in MHCC-97H and SMMC-7721 cells. Furthermore, SLC44A5 knockdown cells also exhibited cell apoptosis by reducing the expression levels of apoptosis markers, caspase-3 and caspase-9 in MHCC-97H and SMMC-7721 cells, and suppressed invasion. The present results suggested that SLC44A5 is involved in HCC carcinogenesis and progression in HCC, indicating that SLC44A5 may be a molecular target in cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Knockdown by shRNA identifies SLC44A5 as a potential therapeutic target in hepatocellular carcinoma

Peng

Ren

et al. 2016

Molecular Medicine Reports

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“…Trimethylamine is an aliphatic amine, which has a potential to generate a highly toxic carcinogen compound N-nitrosodimethylamine, and cause aberrant neurological symptoms in end-stage renal patients [Bain et al 2006]. Choline metabolites were reported as potential biomarkers for prostate cancer diagnosis [Awwad et al 2012]. Ethanolamine is one of the substantial metabolites required for synthesis of two core phospholipids (phosphatidylcholine and phosphatidylethanolamine) which constitute more than 50% of the phospholipid content in eukaryotic membranes [Gibellini and Smith 2010].…”

Section: Metabolic Signatures: Reporter Metabolitesmentioning

confidence: 99%

Molecular signatures of ovarian diseases: Insights from network medicine perspective

Kori

Göv

Arğa

2016

Systems Biology in Reproductive Medicine

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Dysfunctions and disorders in the ovary lead to a host of diseases including ovarian cancer, ovarian endometriosis, and polycystic ovarian syndrome (PCOS). Understanding the molecular mechanisms behind ovarian diseases is a great challenge. In the present study, we performed a meta-analysis of transcriptome data for ovarian cancer, ovarian endometriosis, and PCOS, and integrated the information gained from statistical analysis with genome-scale biological networks (protein-protein interaction, transcriptional regulatory, and metabolic). Comparative and integrative analyses yielded reporter biomolecules (genes, proteins, metabolites, transcription factors, and micro-RNAs), and unique or common signatures at protein, metabolism, and transcription regulation levels, which might be beneficial to uncovering the underlying biological mechanisms behind the diseases. These signatures were mostly associated with formation or initiation of cancer development, and pointed out the potential tendency of PCOS and endometriosis to tumorigenesis. Molecules and pathways related to MAPK signaling, cell cycle, and apoptosis were the mutual determinants in the pathogenesis of all three diseases. To our knowledge, this is the first report that screens these diseases from a network medicine perspective. This study provides signatures which could be considered as potential therapeutic targets and/or as medical prognostic biomarkers in further experimental and clinical studies. Abbreviations DAVID: Database for Annotation, Visualization and Integrated Discovery; DEGs: differentially expressed genes; GEO: Gene Expression Omnibus; KEGG: Kyoto Encyclopedia of Genes and Genomes; LIMMA: Linear Models for Microarray Data; MBRole: Metabolite Biological Role; miRNA: micro-RNA; PCOS: polycystic ovarian syndrome; PPI: protein-protein interaction; RMA: Robust Multi-Array Average; TF: transcription factor.

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“…Choline is a compound that supports the synthesis of cell membranes and thus proliferation. In the case of prostate cancer, it has been strongly related with elevated levels of choline uptake and certain choline metabolites, which can be used as potential prognostic biomarkers for the management of prostate cancer patients (Awwad et al 2012). The Fluorocholine (FCH) molecule labelled with 18 F showed the highest biological compatibility with choline leading to a behaviour in the body very similar to that of natural choline (Hara 2001).…”

Section: Introductionmentioning

confidence: 99%

Impact and correction of the bladder uptake on¹⁸F-FCH PET quantification: a simulation study using the XCAT2 phantom

et al. 2016

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The spill-in counts from neighbouring regions can significantly bias the quantification over small regions close to high activity extended sources. This effect can be a drawback for 18 F-based radiotracers positron emission tomography (PET) when quantitatively evaluating the bladder area for diseases such as prostate cancer. In this work, we use Monte Carlo simulations to investigate the impact of the spill-in counts from the bladder on the quantitative evaluation of prostate cancer when using 18 F-Fluorcholine (FCH) PET and we propose a novel reconstruction-based correction method. Monte Carlo simulations of a modified version of the XCAT2 anthropomorphic

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The role of choline in prostate cancer

Cited by 76 publications

References 65 publications

Knockdown by shRNA identifies SLC44A5 as a potential therapeutic target in hepatocellular carcinoma

Knockdown by shRNA identifies SLC44A5 as a potential therapeutic target in hepatocellular carcinoma

Molecular signatures of ovarian diseases: Insights from network medicine perspective

Impact and correction of the bladder uptake on¹⁸F-FCH PET quantification: a simulation study using the XCAT2 phantom

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The role of choline in prostate cancer

Cited by 76 publications

References 65 publications

Knockdown by shRNA identifies SLC44A5 as a potential therapeutic target in hepatocellular carcinoma

Knockdown by shRNA identifies SLC44A5 as a potential therapeutic target in hepatocellular carcinoma

Molecular signatures of ovarian diseases: Insights from network medicine perspective

Impact and correction of the bladder uptake on18F-FCH PET quantification: a simulation study using the XCAT2 phantom

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Impact and correction of the bladder uptake on¹⁸F-FCH PET quantification: a simulation study using the XCAT2 phantom