2016
DOI: 10.11648/j.cbb.20160401.12
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Abstract: Snake venoms are rich in phospholipase A2 (PLA2) and their hydrolysis of cell membrane phospholipids explains the role of the enzyme in venom toxicity. Calcium is known to plays important role at the active site of PLA2 during catalysis. In this study, molecular dynamics simulations of free PLA2 and calcium bound PLA2 were carried out using GROMACS 4.5.5 to evaluate the role of calcium in PLA2 catalysis. The results showed that calcium induced formation of helical structures between Arg62-Lys66, Asn107-Tyr111 … Show more

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Cited by 3 publications
(2 citation statements)
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“…Catalytic activity and substrate binding of snake venom phospholipases need submicromolar concentrations of calcium ions. Recently, molecular dynamic studies have demonstrated that calcium induces atomistic movements and conformational changes in snake venom PLA 2 which led to the formation of a widened cleft at the active site of calcium bound PLA 2 when compared with free PLA 2 [23]. This ion is also available for binding the substrate phosphate group [22].…”
Section: Discussionmentioning
confidence: 99%
“…Catalytic activity and substrate binding of snake venom phospholipases need submicromolar concentrations of calcium ions. Recently, molecular dynamic studies have demonstrated that calcium induces atomistic movements and conformational changes in snake venom PLA 2 which led to the formation of a widened cleft at the active site of calcium bound PLA 2 when compared with free PLA 2 [23]. This ion is also available for binding the substrate phosphate group [22].…”
Section: Discussionmentioning
confidence: 99%
“…O sítio de ligação dessas enzimas ao Ca 2+ é constituído pelo grupo βcarboxil do resíduo Asp-49 juntamente com o grupo carbonil dos resíduos de tirosina e glicina (DENNIS et al, 2011;FLEER et al, 1981). Após a ligação desses íons, ocorrem mudanças conformacionais e ativação enzimática, essenciais para a hidrólise dos fosfolipídeos, primariamente a fosfatidilcolina (AKUBUGWO et al, 2016;BALSINDE et al, 2002 et al, 1998;BERGH et al, 1989).…”
Section: Fosfolipases a 2 Da Peçonha De Serpentesunclassified