2013
DOI: 10.1189/jlb.0313126
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Abstract: The protein kinase Btk has been implicated in the development, differentiation, and activation of B cells through its role in the BCR and TLR signaling cascades. These receptors and in particular, the BCR and either TLR7 or TLR9 also play a critical role in the activation of autoreactive B cells by RNA- or DNA-associated autoantigens. To explore the role of Btk in the development of autoreactive B cells, as well as their responses to nucleic acid-associated autoantigens, we have now compared Btk-sufficient and… Show more

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Cited by 13 publications
(31 citation statements)
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“…It is possible that the 3-83 BCR may recognize H2-K d , or another unidentified autoantigen, at similar affinity to insulin; therefore, it may share some autoreactive properties with the anti-insulin 125Tg BCR, a viewpoint suggested by previous work regarding receptor editing in the 3-83 model (45). Both of these studies contrast with a new report regarding transgenic AM14 rheumatoid factor B cells, which recognize IgG2a with low affinity ( K d = 2.2 × 10 6 ) (46, 47). Genetic deficiency of BTK in this AM14 model depletes the transgenic autoreactive cells only to the same extent that the endogenous repertoire is depleted, further emphasizing that BTK requirements depend on the nature and affinity of the Ag (46, 47).…”
Section: Discussioncontrasting
confidence: 79%
See 1 more Smart Citation
“…It is possible that the 3-83 BCR may recognize H2-K d , or another unidentified autoantigen, at similar affinity to insulin; therefore, it may share some autoreactive properties with the anti-insulin 125Tg BCR, a viewpoint suggested by previous work regarding receptor editing in the 3-83 model (45). Both of these studies contrast with a new report regarding transgenic AM14 rheumatoid factor B cells, which recognize IgG2a with low affinity ( K d = 2.2 × 10 6 ) (46, 47). Genetic deficiency of BTK in this AM14 model depletes the transgenic autoreactive cells only to the same extent that the endogenous repertoire is depleted, further emphasizing that BTK requirements depend on the nature and affinity of the Ag (46, 47).…”
Section: Discussioncontrasting
confidence: 79%
“…Both of these studies contrast with a new report regarding transgenic AM14 rheumatoid factor B cells, which recognize IgG2a with low affinity ( K d = 2.2 × 10 6 ) (46, 47). Genetic deficiency of BTK in this AM14 model depletes the transgenic autoreactive cells only to the same extent that the endogenous repertoire is depleted, further emphasizing that BTK requirements depend on the nature and affinity of the Ag (46, 47). …”
Section: Discussioncontrasting
confidence: 79%
“…The DNA-reactive autoantibodies spontaneously bind DNA-associated cell debris, released from damaged or dying cells, and facilitate delivery of the endogenous ligand to a TLR9 + endosomal compartment. While these RF B cells have provided an extremely useful experimental model, their application is limited to cells expressing the correct BCR transgene, requiring extensive intercrossing to evaluate multi-gene genetically targeted mice (16). …”
Section: Resultsmentioning
confidence: 99%
“…B cells were purified and stimulated as described previously (16) with goat anti-mouse IgM F(ab′) 2 (Jackson ImmunoResearch), ODN 1826 (CpG; Idera Pharmaceuticals), anti-DNA-mAb (4) or DVD-Ig proteins (1 ug/ml). Proliferation was assessed by 3 H-thymidine (Amersham Biosciences, Piscataway, NJ, USA) incorporation at 24 hr post-stimulation.…”
Section: Methodsmentioning
confidence: 99%
“…AM14 Vκ8R, AM14 Vκ8R Tlr7 -/-, AM14 Vκ8R Tlr9 -/-, and AM14 Vκ8R Tlr7/9 -/-mice were described previously (10,19,45,46). DO11.10 Tcrα -/-BALB/c mice were previously described (18).…”
Section: Methodsmentioning
confidence: 99%